Single-fiber-type CARS excitation source device and realization method based on two-stage non-linear tuning

Abstract

The invention discloses a single-fiber-type CARS excitation source device and realization method based on two-stage non-linear tuning. The device comprises a femtosecond fiber laser light source, an electric control light power attenuator, a first-stage tuner, a pulse broadening device, a second-stage tuner, a pump laser, a wavelength division multiplexer, a gain fiber, an optical filter and an output port. The femtosecond fiber laser light source outputs femtosecond pulses serving as Stokes optical pulses which enter the electric control light power attenuator; power control optical pulse wavelength tuning is realized in the first-stage tuner; the pulses are broadened to be femtosecond pulses through the pulse broadening device, and the femtosecond pulses then enter the gain fiber for amplification; signal optical pulses and idler frequency optical pulses are generated in the second-stage tuner, wherein the signal optical pulses serve as pump optical pulses of a CARS source; the optical filter filters the idler frequency optical pulses and the residual pump continuous light; and the rest optical pulses are output from the output port. Compared with the prior art, the device adopts a single-fiber light path structure and electric control tuning, and has the advantages of compact structure, anti-interference, high reliability and fast tuning speed; and CARS rapid imaging requirement is met.

Description

technical field [0001] The invention relates to the technical field of optical nonlinear microscopic imaging, in particular to an excitation source device of a CARS microscopic imaging system. Background technique [0002] CARS microscopy utilizes coherent anti-Stokes Raman scattering to detect molecules. When the frequency difference between the pump light and the Stokes light is equal to the vibration frequency of the molecule to be detected, strong anti-Stokes scattered light will be generated, thereby enabling the detection of the molecule to be detected. Due to the high signal-to-noise ratio of CARS microscopic imaging technology, its three-dimensional tomographic capability, and the need for fluorescent labeling and confocal systems, it has attracted widespread attention in recent years and has developed rapidly. The CARS microscopy imaging system requires two wavelengths of laser light, at least one of which is wavelength tunable. At present, CARS systems usually us...

AI Technical Summary

Problems solved by technology

At present, CARS systems usually use two Ti-doped sapphire lasers with feedback control or two optical parametric oscillators pumped by the same pump source as the excitation source. The pump light and the Stokes light need to be synchronized, and the adjustment is complicated and requires The tuning of the mechanical device leads to slow wavelength tuning, and the entire system is huge, requiring full-time technicians to maintain it, which seriously hinders the wide application of CARS

Images