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Thin layer chromatographic identification method of matrine and oxymatrine in sophora flavescens

A technology of oxymatrine and thin-layer chromatography, which is applied in the field of identification of Chinese herbal medicines, can solve problems such as unsatisfactory chromatographic results, troublesome and time-consuming secondary development of a biphasic developer, and achieve the goal of avoiding insufficient color development and good color development. Color stability, precise effect of unfolding effect

Inactive Publication Date: 2015-08-12
HENAN KANGXING PHARMA +2
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operation process of this method is too cumbersome and time-consuming, especially the chromatographic results are not ideal; The phenomenon is serious; using bismuth potassium iodide test solution as the color developing agent, the color develops quickly, but the spots disappear too fast, which is not conducive to maintaining the image data
In addition, the actual application effect is not good due to the deviation of the long-term measurement results of thin-layer chromatography and the inconspicuous color development.

Method used

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  • Thin layer chromatographic identification method of matrine and oxymatrine in sophora flavescens
  • Thin layer chromatographic identification method of matrine and oxymatrine in sophora flavescens
  • Thin layer chromatographic identification method of matrine and oxymatrine in sophora flavescens

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Embodiment 1

[0023] (1) Take matrine reference substance and oxymatrine reference substance, add ethanol respectively to make a solution containing 0.2-0.3mg matrine reference substance or oxymatrine reference substance per 1mL of ethanol, and use as matrine reference substance Alkaline reference substance solution and oxymatrine reference substance solution;

[0024] (2) Take 0.3-0.5g of superfine powder of Sophora flavescens medicinal material, add 0.25-0.3mL of concentrated ammonia water and 20-25mL of chloroform mixed solution, 250W ultrasonic for 15-25min, filter, evaporate the filtrate to dryness on a water bath, add ethanol to the residue 0.4 ~ 0.5mL to dissolve, as the test solution;

[0025] (3) Take 3-4uL each of the matrine reference solution and the test solution, and place samples on different positions on the same horizontal line of the same silica gel H thin-layer plate in sequence, using chloroform-methanol-concentrated ammonia-toluene test solution as the developer , the ...

Embodiment 2

[0034] (1) Take matrine reference substance and oxymatrine reference substance, and add ethanol respectively to make a solution containing 0.25-0.28 mg of matrine reference substance or oxymatrine reference substance per 1 mL of ethanol, which are used as matrine reference substance Alkaline reference substance solution and oxymatrine reference substance solution;

[0035] (2) Take 0.4-0.5g of superfine powder of Sophora flavescens, add 0.3mL of concentrated ammonia and 25mL of chloroform to a mixed solution, 250W ultrasonic for 20-25min, filter, evaporate the filtrate to dryness on a water bath, add 0.5mL of ethanol to dissolve the residue , as the test solution;

[0036] (3) Take 3-4uL each of the matrine reference solution and the test solution, and place samples on different positions on the same horizontal line of the same silica gel H thin-layer plate in sequence, using chloroform-methanol-concentrated ammonia-toluene test solution as the developer , the mass ratio of t...

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Abstract

The invention discloses an improved thin layer chromatographic identification method of matrine and oxymatrine in sophora flavescens. In the improved thin layer chromatographic method, concentrated ammonia liquor and trichloromethane ultrasonic extraction is adopted in the preparation of sample, different developing systems are used to carry out thin layer chromatographic identification on matrine and oxymatrine respectively; at the same time, an improved bismuth-potassium iodide reagent is used as the developing agent, and under the sunlight, the sample can generate a spot with the same color in a corresponding position of a reference in the thin layer chromatography. The identification method has the advantages of clear spot and good repeatability, and is capable of precisely identifying two characteristic components of sophora flavescens namely matrine and oxymatrine at the same time. Compared with the classic method provided by the Chinese pharmacopoeia (2010 edition), the provided method has the advantages of simple and fast treatment, clear spot, moderate developing time, and good repeatability.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicinal materials, in particular to a method for quickly and accurately identifying two characteristic components of matrine and oxymatrine in flavescens medicinal materials by using an improved thin-layer chromatography identification method. Background technique [0002] Sophora flavescens is a commonly used traditional Chinese medicine, derived from the dried root of the leguminous plant Sophora flavescens Sop hor a f lavesens Ait. It has heat-clearing, damp-drying and insecticidal effects. One of the ingredients has anti-tumor, anti-asthma and antibacterial effects; oxymatrine has anti-tumor, anti-asthma and other effects. Therefore, the identification of matrine and oxymatrine in flavescens is the key to the quality control of flavescens. [0003] The TLC identification of matrine and oxymatrine in the 2010 edition of "The Pharmacopoeia of the People's Republi...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 张国祖马霞李宇伟金燕飞王兴涛刘梅
Owner HENAN KANGXING PHARMA
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