A constructing method of a bone marrow stem cell expressing an osteogenic gene
A bone marrow mesenchymal and osteogenic gene technology, applied in genetic engineering, plant gene improvement, botanical equipment and methods, etc., to achieve the effects of good cell growth, simple operation, and improved expression level
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[0019] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0020] The lentiviral vector is obtained by transformation of human immunodeficiency virus (humanimmunodeficiency virus, HIV). The lentiviral vector system includes expression vector and packaging plasmid. Integrate the required genetic information. On the basis of HIV, the four auxiliary genes vif, vpr, vpu and nef were deleted, and at the same time, a plasmid expressing vesicular stomatitis virus (VSV) glycoprotein G and amphotropic murine leukemia virus (MLV) envelope protein Env were used The plasmids that express the HIV-1 envelope protein Env are replaced re...
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