Complex microbial inoculant for degradation of chlorohydrocarbon and application thereof

A compound bacterial agent and chlorinated hydrocarbon technology, applied in the field of microorganisms, can solve the problems of incomplete degradation and product toxicity, and achieve the effects of good adaptability, anti-pollution ability and good tolerance.

Inactive Publication Date: 2015-08-12
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to address the deficiencies in the prior art, to provide a compound bacterial agent for degrading chlorinated hydrocarbons, which can achieve efficient degradation of refractor...

Method used

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  • Complex microbial inoculant for degradation of chlorohydrocarbon and application thereof
  • Complex microbial inoculant for degradation of chlorohydrocarbon and application thereof
  • Complex microbial inoculant for degradation of chlorohydrocarbon and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Step 1) acclimatization of bacterial classification

[0048] Methylocystis sp.JTA1 directional domestication of chlorinated hydrocarbons: Garbage particles are selected from the mineralized waste of domestic waste landfills. This embodiment selects the Shanghai Laogang landfill (east of Laogang, Nanhui District, Shanghai) and has filled it. Buried garbage for 10 years, took garbage particles under 4mm sieve and 2mm sieve, added a certain amount of chloroform and adjusted the pH to be suitable for the growth of Methylocystis sp. Rejuvenation of the strain is achieved.

[0049] Methylobacterium sp.R1 chlorinated directional domestication: Garbage particles are selected from the overburden of domestic waste landfills. In this embodiment, Chongqing Changshengqiao Landfill (Nan'an District, Chongqing City) is selected, and the 4mm sieve and the 2mm sieve are taken. Garbage particles, add a certain amount of chloroform and adjust the pH to be suitable for the growth of Methy...

Embodiment 2

[0064] Embodiment 2: Composite bacteria agent is to the degradation of different concentrations trichlorethylene

[0065] 1. Take the composite bacterial agent (OD 600 =0.6±0.1) Put 20ml into a 100ml serum bottle, seal it with a polytetrafluoroethylene rubber stopper lined with it, and seal it with an aluminum cap, shake and culture at 30°C and 170r / min for 24h, and detect trichlorethylene by gas chromatography The degradation rate was 95%, which indicated that the composite bacterial agent had good trichlorethylene tolerance and degradation effect.

[0066] 2. Take the composite bacterial agent (OD 600 =0.6±0.1) Put 20ml into a 100ml serum bottle, seal it with a polytetrafluoroethylene rubber stopper lined with it, and seal it with an aluminum cap, shake and culture at 30°C and 170r / min for 24h, and detect trichlorethylene by gas chromatography The degradation rate was 96%, which indicated that the composite bacterial agent had good trichlorethylene tolerance and degradatio...

Embodiment 3

[0069] The histogram of the degradation of different concentrations of trichlorethylene by the compound bacterial agent is as follows figure 2 As shown, it shows that the composite bacterial agent has good tolerance and degradation effect under the condition that the concentration of trichlorethylene is less than 8mg / L, when the concentration of trichlorethylene is 1.65mg / L, 2.73mg / L and 5.39mg / L , the degradation rates reached 95%, 96% and 97% respectively. Example 3: Degradation of different types of chlorinated hydrocarbons in wastewater by composite bacterial agents

[0070] 1. Take the compound bacterial agent (OD 600 =0.6±0.1) Put 20ml into a 100ml serum bottle, seal it with a PTFE-lined rubber stopper, and seal it with an aluminum cap, shake and culture at 30°C and 170r / min for 6h, and detect with gas chromatography 1,3 - The degradation rate of trichloropropane is 0.03mg / (L*h), and the degradation rate reaches over 90% after 2 days of cultivation, indicating that th...

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Abstract

The present invention relates to a complex microbial inoculant for degradation of chlorohydrocarbon and application thereof. The complex microbial inoculant includes 10-30% of methylocystis sp.JTA, 20-40% of methylobacterium sp.R1, 10-30% of microbacterium sp.DH and 5-20% of cupriavidus sp.SWA1. The invention employs co-metabolism and direct oxidation of a variety of microorganisms to achieve efficient degradation on the chlorohydrocarbon hard to degrade, effectively solves the problems of incomplete degradation or higher toxicity of product after dechlorination in the anaerobic degradation of chlorohydrocarbon, and is expected to achieve new breakthroughs in engineering application of chlorohydrocarbon biodegradation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a composite bacterial agent for degrading chlorinated hydrocarbons and its application. Background technique [0002] As important organic solvents and product intermediates, chlorinated hydrocarbons are widely used in chemical, pharmaceutical, pesticide and other fields. Due to improper use and storage, chlorinated hydrocarbons are discharged into the natural environment through volatilization, leakage, and wastewater discharge. A large number of studies in recent years have shown that chlorinated hydrocarbon pollutants such as trichlorethylene have potential "three-cause" (carcinogenic, teratogenic, mutagenic) effects and genotoxic effects. And central nervous system damage, causing a series of symptoms of body dysfunction, seriously endangering human health. With international and domestic demands on the ecological environment becoming higher and higher in sustainable...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/36
Inventor 赵天涛袁建华李雷何芝杨旭
Owner CHONGQING UNIV OF TECH
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