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Rhizosphere Growth Promoting Bacteria and Its Application

A technology of rhizosphere growth-promoting bacteria and microbial strains, applied in the field of agricultural microorganisms, can solve problems such as threats to human health and living environment, polluted soil, water sources, and food itself, achieving significant growth-promoting effects, promoting growth-promoting characteristics, Growth-promoting effect

Active Publication Date: 2017-12-19
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In agricultural production, the use of chemical fertilizers increases the yield of wheat, but it also releases a large amount of harmful substances to the environment, pollutes the soil, water sources and food itself, and poses a great threat to human health and the living environment

Method used

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  • Rhizosphere Growth Promoting Bacteria and Its Application
  • Rhizosphere Growth Promoting Bacteria and Its Application
  • Rhizosphere Growth Promoting Bacteria and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Identification of strains

[0032] The rhizosphere growth-promoting bacteria N10 was expanded and cultured on LB medium in a 28°C incubator, and the characteristics of the colony were observed: the top of the colony was flat, raised, translucent, milky white, and the surface was relatively moist.

[0033] The rhizosphere growth-promoting bacterium is the rhizosphere growth-promoting bacterium N10, and its classification is named as Enterobacter cancerogenus (Enterobactercancerogenus), which was preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee on March 25, 2015. The deposit number is CGMCC No.1.14969.

[0034] Pick the colonies to collect the bacteria, use the Tiangen Bacteria Genomic DNA Extraction Kit to extract the genomic DNA of the strains according to the enclosed instructions, and store them in a -20°C refrigerator for later use.

[0035] 16S rRNA gene PCR:

[0036] ① Primer 27F: AGA GTT TGA TC...

Embodiment 2

[0051] Acquisition of rhizosphere growth-promoting bacteria N10 and determination of its growth-promoting properties:

[0052] Weigh 1 g of Jerusalem artichoke rhizosphere soil sample into a Erlenmeyer flask filled with 99 mL of sterile water, shake it on a shaker for 30 min, and then let it stand for 10 min. Take 1ml of the supernatant and shake it evenly in a test tube filled with 9ml of sterile water, the concentration is 10 -3 . and so on, do 10 -4 ,10 -5 ,10 -6 ,10 -7 A total of 5 dilution gradients. Draw 100 μL respectively and spread on nitrogen-free Ashby medium, and culture at 28°C for 3-4 days. Pick a single colony for purification, microscopic examination, and preserve it on a slant. Alternately streak on LB medium and nitrogen-free Ashby medium plate and transfer 7 to 8 times. The strains that can grow normally are nitrogen-fixing strains, and they are stored in a -80°C refrigerator with 20% glycerol for the next test.

[0053] Nitrogen-free Ashby medium (1...

Embodiment 3

[0074] Example 3: Wheat inoculation test of the strain.

[0075] ① Soak the wheat seeds in 10% (v / v) sodium hypochlorite for 10 minutes to disinfect the surface, wash them with sterile water 5 times, incubate and germinate, and transplant the seeds with the same growth status to the sterilized container mixed with low nitrogen- In the double-layer pots of the vermiculite of the calcium phosphate culture solution, each one in each double-layer pot, totally 10 pots.

[0076] ②Acclimate and culture Enterobacter carcinogenes in LB medium, expand the culture at 28°C, when the bacterial concentration is OD 600 When = 0.8, each draw 1mL bacterial solution and spray on the surface of 5 pots of germinated wheat seeds; while the other 5 pots each spray 1mL sterile LB medium as a control. ③Cultivate in the greenhouse, the temperature is controlled at 26±2°C, the photoperiod is 12h:12h, and the whole treatment follows a completely randomized block design.

[0077] After 50 days, the aer...

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Abstract

The invention relates to the field of agricultural microorganisms, in particular to a rhizosphere growth-promoting bacterium and its application. The rhizosphere growth-promoting bacterium is the rhizosphere growth-promoting bacteria N10, and its classification is named Enterobacter cancerogenus (Enterobacter cancerogenus), which was preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC) on March 25, 2015. The number is CGMCC No.1.14969. The bacterial strain of the present invention has the growth-promoting characteristics of nitrogen fixation, phosphorus dissolution, and indole acetic acid production, can increase soil nutrients, improve the effectiveness of fertilizers, and promote the absorption of soil nutrients by plants. Good prospects. The invention has a remarkable growth-promoting effect on wheat, and the good growth-promoting characteristics of the bacterial strain promote the growth of the aboveground parts of wheat.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to a rhizosphere growth-promoting bacterium and its application. Background technique [0002] Wheat is a grass plant, the most widely distributed grain crop in the world, and its sown area is the crown of grain crops. In 2010, its total output in the world reached 651 million tons, second only to 844 million tons of corn. At the same time, wheat is one of the most important rations in China. It is mainly produced in Henan, Shandong, Jiangsu, Hebei, Hubei, Anhui and other provinces. The development of the wheat industry is directly related to national food security and social stability. However, the improvement effect of science and technology on this crop in our country is far lower than the reduction of planting area. [0003] In agricultural production, the use of chemical fertilizers increases the yield of wheat, but it also releases a large amount of harmful substan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08
Inventor 解志红李项岳李岩宫江峰姜楠
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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