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Bacillus subtilis and preparation method and application thereof

A technology for Bacillus subtilis and Bacillus subtilis, which is applied to the field of Bacillus subtilis and its preparation, can solve the problems of high separation speed, low yield, and no registration, and achieves the effects of short fermentation period, high yield and convenient operation.

Inactive Publication Date: 2015-07-29
德强生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. The existing Bacillus subtilis preparations have obvious control effects on plant diseases caused by various fungi such as rice blast, rice false smut, cucumber powdery mildew, strawberry gray mold, etc., but there is no manufacturer on the diseases caused by bacteria Get registered
[0006] 2. The existing method for preparing Bacillus subtilis usually adopts liquid fermentation to obtain fermented liquid, concentrates with a disc centrifuge after pretreatment, and then sprays and dries to obtain the original powder; the fermented liquid is separated through a disc centrifuge, and the heavy liquid is left. The light liquid is discarded, the separation speed is fast but the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] The preparation method of Bacillus subtilis of the present invention comprises the following steps:

[0037] S010, prepared strains

[0038] That is to say, 10 Kelvin flask seeds are added with sterile water to make a bacterial suspension, which is inoculated into a large tank under aseptic conditions;

[0039] S020, fermentation medium

[0040] That is, beer yeast powder with a content of 2.0% to 3.2% and a fineness greater than 80 meshes, industrial grade corn starch with a content of 1.0% to 1.6%, industrial grade calcium carbonate with a content of 0.2% to 0.6%, and a content of 0.2% to 0.6% % of industrial grade potassium dihydrogen phosphate, 0.2% to 0.6% of industrial grade ammonium sulfate and 0.02% to 0.04% of industrial grade defoamer are placed in a tank whose volume after sterilization is 60% of the volume of the fermentation tank , pH 7.3~7.5 before sterilization,

[0041] Then, keep the ventilation rate at 1:0.6 during the period of 0-6 hours, keep the ...

Embodiment 2

[0053] Tobacco bacterial wilt (carried out two years and four field field efficacy tests, the results are excerpted as follows):

[0054] In 2011, Guizhou Provincial Pesticide Control Institute used 600, 750, and 900 g / ha of pesticides, and the control effects were 44.65%, 62.73%, and 70.23% after 14 days of spraying the base of the stem twice, and the control agent was 72% Agricultural streptomycin soluble powder The control effect of 540mg / kg is 61.57%. The dosage of the preparation of the Agricultural Environmental Resources Research Institute of the Yunnan Academy of Agricultural Sciences is 600, 750, and 900 grams per hectare. / ha, the control effect is 71.00%. Henan Provincial Pesticide Control Institute’s dosage of 600, 750, 900 g / ha sprayed the base of the stem twice and the 14-day control effect was 61.36%, 65.03%, 67.39%, respectively, and the control agent was 300 billion / piece of Pseudomonas fluorescens powder 8812.5 g / ha, the control effect is 64.43%. Shandong ...

Embodiment 3

[0057] Tobacco wildfire disease (field efficacy trials conducted in two years and four places, the results are excerpted below):

[0058] In 2011, Guizhou Provincial Pesticide Control Institute used 600, 750, and 900 g / ha of preparations, and the control effects were 65.83%, 76.03%, and 81.70% after 1 spray for 14 days. The powder is 750 g / ha, and the control effect is 75.57%. The dosage of 600, 750, and 900 g / ha of the preparation of the Institute of Agricultural Environment and Resources of the Yunnan Academy of Agricultural Sciences is 53.87%, 63.04%, and 70.44%, respectively, and the control effect after spraying 3 times is 1250 g of the 77% copper sulfate calcium wettable powder preparation / ha, the control effect is 72.54%. Henan Provincial Pesticide Control Institute preparation dosage 600, 750, 900 g / ha sprayed 2 times and the 14-day control effect was 71.90%, 74.23%, 76.68% respectively, compared with 50% chlorobromoisocyanuric acid soluble powder 525 g / ha hectares,...

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Abstract

The invention relates to bacillus subtilis. The preservation number of the strain is CGMCC NO: 9269. The strain is obtained by deep fermentation, ceramic membrane concentration and spray-drying. A shaking culture liquid of the strain has an obvious effect of inhibiting the target germ Xanthomonas oryzae pv. oryzae, the target germ ralstonia solannacearum, the target germ Pseudomonas syringae pv. tabaci, the target germ Xanthomonas axonopodis pv. citri and the target germ cercosporella albomaculans. The bacillus subtilis provided by the invention has advantages of fast growth and propagation, short fermentation period, high filling spore number, good control efficiency and the like. Meanwhile, a preparation method of the strain has simple steps, is convenient to operate, comprises steps of deep fermentation, ceramic membrane concentration and spray-drying to obtain the bacillus subtilis, has high preparation efficiency and has good effects. The strain can control various bacterial diseases and now has been registered on cercosporella albomaculans, Xanthomonas axonopodis pv. citri, Pseudomonas syringae pv. tabaci, ralstonia solannacearum and Xanthomonas oryzae pv. oryzae.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacillus subtilis and its preparation method and application. Background technique [0002] Bacterial disease is a kind of major disease that endangers the growth of crops, and has always seriously endangered agricultural production; at present, the commonly used agents to prevent and control bacterial diseases are mainly chemical pesticides, and the control effect on some diseases is general, and it is harmful to the ecological environment and the quality of agricultural products. The security implications are huge. In my country, there is still a lack of high-efficiency, broad-spectrum, low-toxicity, and low-residue biopesticides mainly for the prevention and treatment of bacterial diseases. [0003] Bacillus subtilis is a kind of Bacillus, its single cell is 0.7~0.8×2~3 microns, and the coloring is uniform. Uncapsulated, with periosteum flagella, able to move. Gram-positive b...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/125
CPCA01N63/10C12N1/20C12N1/205C12R2001/125
Inventor 易虹王敏杨文亮章丽田利崔占东穆春董蕾余满
Owner 德强生物股份有限公司
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