Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting cyclic adenosine monophosphate from fermentation liquid

A technology of cyclic adenosine monophosphate and fermented liquid, which is applied in the direction of chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of unfavorable industrial production, expensive raw materials, waste of the environment, etc., and achieve environmental protection, Reduce production costs and benefit wastewater treatment

Inactive Publication Date: 2015-07-22
XINYU PHARM CO LTD
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main production method of cyclic adenosine monophosphate is chemical synthesis, which has complex process, expensive and difficult to obtain raw materials, many by-products, low yield, high cost, small output, will cause environmental pollution, and is not conducive to industrial production, etc. shortcoming
[0004] The use of microbial fermentation technology to produce cyclic adenosine monophosphate is low in cost, easy to treat wastewater, and is conducive to environmental protection. The existing microbial production of cyclic adenosine monophosphate mainly uses ion exchange and resin chromatography processes, which are high in cost and large in water consumption. Eluted with formic acid, followed by NH 4 OH neutralization, direct discharge is wasteful and causes environmental pollution. To purify cAMP by thin-layer chromatography, it is necessary to scrape off the cAMP zone on the silica gel plate and then separate cAMP. Not only is the operation cumbersome, but it is also not suitable for industrial production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of fermentation broth

[0024] The fermented liquid used in the present invention is Arthrobacter fermented liquid, wherein the strain number is cAMP091015, which is preserved in the strain room of our company.

[0025] The concrete composition of medium used in this embodiment is as follows:

[0026] Incline medium (g / 100ml): potato juice 10, peptone 1.0, magnesium sulfate 0.001, guanine 0.05, agar 2.0, pH 7.0.

[0027] Seed medium (g / 100ml): glucose 1.0, yeast extract 1.0, peptone 1.0, beef extract 1.0, urea 0.6, sodium chloride 0.2, pH 7.0.

[0028] Fermentation medium (g / 100ml): glucose 4.5, dipotassium hydrogen phosphate 1.0, potassium dihydrogen phosphate 1.0, magnesium sulfate 0.5, biotin 0.005, cobalt chloride 0.001, hypoxanthine 0.006, etc., pH 7.0.

[0029] The fermentation conditions are as follows:

[0030] Inoculate the strains stored in the refrigerator at 1-4°C to the slant medium for activation, and culture at 30°C for 24 hours. The bacte...

Embodiment 2

[0032] This embodiment provides a method for extracting cyclic adenosine monophosphate from the fermentation broth, the operation steps are as follows:

[0033] 1. Adopt 1 L of the fermented liquid prepared in Example 1, the fermented liquid unit is 4 g / L, heat the fermented liquid to 70° C. and pass through a ceramic membrane with a pore size of 0.02--0.05 μm to obtain an ultrafiltrate;

[0034] 2. Collect the ultrafiltrate prepared in step 1, adjust the pH to 7.05 with ammonia water, add ammonia water to elute and analyze after passing through the carbon chromatography column to obtain the eluent, and the activated carbon used in the carbon chromatography column is shell-type activated carbon ;

[0035] 3. Adjust the pH of the eluate obtained in step 2 to 7.05 with ammonia water, concentrate to one-fifth of the initial volume under the conditions of vacuum degree 0.075-0.1Mpa, concentration temperature 50-60°C, and add the volume of the concentrated solution to the concentr...

Embodiment 3

[0040] This embodiment provides a method for extracting cyclic adenosine monophosphate from the fermentation broth, the operation steps are as follows:

[0041] 1. Adopt 1 L of the fermented liquid prepared in Example 1, the fermented liquid unit is 4 g / L, heat the fermented liquid to 70° C. and pass through a ceramic membrane with a pore size of 0.02--0.05 μm to obtain an ultrafiltrate;

[0042] 2. Collect the ultrafiltrate prepared in step 1, adjust the pH to 7.5 with ammonia water, add ammonia water for elution and analysis after passing through the carbon chromatography column to obtain the eluent, and the activated carbon used in the carbon chromatography column is shell-type activated carbon;

[0043] 3. Adjust the pH of the eluate obtained in step 2 to 7.5 with ammonia water, concentrate to one-sixth of the initial volume under the conditions of a vacuum of 0.075-0.1Mpa and a concentration temperature of 50-60°C, and add the volume of the concentrated solution to the co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for extracting cyclic adenosine monophosphate from a fermentation liquid, and belongs to the technical field of biological active substance extraction. The method comprises the following steps: heating the fermentation liquid, and filtering the fermentation liquid through a membrane to obtain an ultra-filtrate; adjusting the pH of the ultra-filtrate to be alkaline, and enabling the ultra-filtrate to pass through a carbon chromatography column for adsorbing; eluting by using ammonia water; collecting an eluting solution; adjusting the pH of the eluting solution to be alkaline; vacuumizing at 55 to 60 DEG C and performing suction filtration; adjusting the pH of the filtrate to be acidic; adding methyl alcohol in a flowing manner for crystallizing; drying crystals in vacuum to obtain crude cyclic adenosine monophosphate; re-crystallizing the crude cyclic adenosine monophosphate to obtain finished cyclic adenosine monophosphate. The cyclic adenosine monophosphate prepared by the method is stable in quality; the purity is up to 99 percent; the yield is up to 75 percent; a traditional ion exchange and resin chromatography process is replaced by adopting a carbon chromatographic method; the production cost is reduced; ammonia water eluting is favorable to treat wastewater.

Description

technical field [0001] The invention belongs to the technical field of extraction of microbial fermentation products, in particular to a method for extracting cyclic adenosine monophosphate from fermentation broth. Background technique [0002] Cyclic adenosine monophosphate is adenosine 3',5'-cyclic phosphate, referred to as cAMP, which is a substance that exists widely in the human body and has important physiological activities. Glycosides are catalyzed by adenosine activating enzymes. As a second messenger substance involved in the regulation of cell functions, it plays a role in hormone regulation of physiological functions and substance metabolism in cells, and plays an important role in sugar, fat metabolism, synthesis and regulation of nucleic acid and protein. It can enhance myocardial contractility, cause blood pressure to increase, and increase cardiac output. It can relax smooth muscle, expand coronary vessels, improve liver function, promote nerve regeneration...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/213C07H1/06
CPCC07H19/20C07H1/06
Inventor 任启伟秦艳飞蔡慧丽李为全刘瑞华孟咏梅张淑珍
Owner XINYU PHARM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com