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Biological trichoderma viride granule as well as preparation method and application thereof

A technology of biological particles and Trichoderma, which is applied in the application, fertilizer mixture, fertilization device and other directions, can solve the problems of conidia activity and seed germination, so as to solve soil-borne diseases and continuous cropping obstacles, inhibit growth and market prospects. expansive effect

Active Publication Date: 2015-07-22
浙江华智生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Trichoderma wettable powder of Shandong Tainuo Pharmaceutical Co., Ltd. is mainly registered in the control of vegetable gray mold), and this wettable powder is not suitable for treating soil and corn seed coating. On the one hand, the current Trichoderma wettable powder is mainly in the form of Environmentally sensitive conidia are the main components. On the other hand, direct treatment of corn seeds with Trichoderma wettable powder is likely to affect seed germination, and the activity of Trichoderma conidia is easily affected by the chemical seed coating agent coated on the surface of the seeds. (Most of all commercially sold corn seeds in China have been coated with various chemical seed coating agents)

Method used

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  • Biological trichoderma viride granule as well as preparation method and application thereof
  • Biological trichoderma viride granule as well as preparation method and application thereof
  • Biological trichoderma viride granule as well as preparation method and application thereof

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Embodiment 1

[0047] The present embodiment provides a kind of preparation method of Trichoderma biological granule, specifically comprises the following steps:

[0048] 1. Plate strains: inoculate Trichoderma aculeatus ZJSX5003 on a PDA medium plate in a 28°C incubator; invert for 2 to 3 days.

[0049] PDA medium: 200g potatoes cut into 1cm 3 Small pieces, boiled on low heat for 30 minutes, filtered through 4 layers of gauze to retain the juice; add 20 grams of glucose, 20 grams of agar powder, distilled water to 1 liter, and autoclave at 121 ° C for 30 minutes.

[0050] 2. Preparation of secondary strains: Take 200 grams of potatoes, 20 grams of glucose, and 1 liter of water to prepare a secondary fermentation culture solution, adjust the pH value to 6-8, and distribute 100 milliliters in 250 milliliter triangular flasks. Sterilize at 121°C for 30 minutes, insert plate strains, shaker speed is 180 rpm, fermentation temperature is 28°C, fermentation time is 3 days, and a large number of d...

Embodiment 2

[0063] The present embodiment provides a kind of preparation method of Trichoderma biological granule, specifically comprises the following steps:

[0064] 1. Plate strain: Inoculate Trichoderma aculeatus ZJSX5003 on a PDA medium plate, in an incubator at 28°C, and culture it upside down for 2 to 3 days.

[0065] PDA medium: 200g potatoes cut into 1cm 3 Small pieces, boiled on low heat for 30 minutes, filtered through 4 layers of gauze, retained the juice, added 20 grams of glucose, 20 grams of agar powder, distilled water to 1 liter, and sterilized by high-pressure steam at 121 ° C for 30 minutes.

[0066] 2. Preparation of secondary strains: Take 200 grams of potatoes, 20 grams of glucose, and 1 liter of water to prepare a secondary fermentation culture solution, adjust the pH value to 6-8, and distribute 100 milliliters in 250 milliliter triangular flasks. Sterilize at 121°C for 30 minutes, insert plate strains, shaker speed is 180 rpm, fermentation temperature is 28°C, fe...

Embodiment 3

[0076] 1. Plate strains: inoculate Trichoderma aculeatus ZJSX5003 on a PDA medium plate in a 28°C incubator; invert for 2 to 3 days.

[0077] PDA medium: 200g potatoes cut into 1cm 3 Small pieces, boiled on low heat for 30 minutes, filtered through 4 layers of gauze to retain the juice; add 20 grams of glucose, 20 grams of agar powder, distilled water to 1 liter, and autoclave at 121 ° C for 30 minutes.

[0078] 2. Preparation of secondary strains: Take 200 grams of potatoes, 20 grams of glucose, and 1 liter of water to prepare a secondary fermentation culture solution, adjust the pH value to 6-8, and distribute 100 milliliters in 250 milliliter triangular flasks. Sterilize at 121°C for 30 minutes, insert plate strains, shaker speed is 180 rpm, fermentation temperature is 28°C, fermentation time is 3 days, and a large number of divergent flocculent hyphae and dispersed conidia liquid bacteria are obtained kind.

[0079] 3. Preparation of Trichoderma liquid fermentation broth...

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Abstract

The invention discloses a trichoderma viride granule as well as a preparation method and application thereof. The trichoderma viride granule comprises the following components in percentage by weight: 30-40 percent of trichoderma viride liquid fermentation liquid, 5-10 percent of corn flour, 30-40 percent of diatomite, 10-20 percent of wheat bran, 1-2.0 percent of zinc sulfate fertilizer, 0.4-0.6 percent of humic acid and 0.4-0.6 percent of nitrogen phosphorus potassium compound fertilizer. The biological granule has the advantages of high antagonism and high specificity, the com stalk rot can be effectively prevented by using the granule, and multiple coordination effects of restraining germination of pathogenic bacteria in soil, promoting breeding of trichoderma viride and promoting crop root growth are achieved.

Description

technical field [0001] The invention relates to a biocontrol agent, in particular to a Trichoderma biological granule and its preparation method and application, in particular to a preparation method of a Trichoderma biological granule for preventing and treating corn stalk rot and its control application. Background technique [0002] Trichoderma viride is a widely used biological control microorganism in the world. As an antagonistic fungus with important biocontrol value, it has been found to have antagonistic effect on plant pathogenic fungi as early as the 1930s. Its mechanism of action includes antibacterial effect, reparasitic effect, bacteriolysis, competition effect and so on. [0003] Corn is the largest food crop in my country. Corn stalk rot is a kind of soil-borne disease that seriously threatens corn production, causing serious lodging and yield loss. At present, the method of chemical pesticide seed dressing is mainly used for the prevention and control of thi...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 吴晓儒陈捷李雅乾
Owner 浙江华智生物科技有限公司
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