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A preparing method of a nanometer polymer micelle carrier integrating diagnosis and treatment

A nano-polymer and micelle technology, applied in the field of preparation of bifunctional nano-polymer micelle system, can solve the problems of allergic reaction, toxic reaction and the like, and achieve the effect of improving local concentration, simple operation and easy implementation

Inactive Publication Date: 2015-07-22
王巧英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for preparing a nano-polymer micelle carrier integrating diagnosis and treatment, so as to overcome the problem of allergic reactions and toxic reactions easily caused by hydrophobic antineoplastic drugs in the prior art, which are serious Deficiencies that limit clinical use

Method used

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  • A preparing method of a nanometer polymer micelle carrier integrating diagnosis and treatment
  • A preparing method of a nanometer polymer micelle carrier integrating diagnosis and treatment
  • A preparing method of a nanometer polymer micelle carrier integrating diagnosis and treatment

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preparation example Construction

[0041] (4) Preparation of FITC-PEG-PCL

[0042] 1-5 parts by weight of NH 2 - PEG-PCL is dissolved in tetrahydrofuran solution, under ultrasonic conditions, drop into 5-10 parts by weight of PH=10 aqueous solution to prepare a micellar solution, then add 1500-4000 parts by weight of FITC, and stir at room temperature 24-48h, and then dialyzed with deionized water (MWO=100kDa), and freeze-dried to obtain FITC-PEG-PCL.

Embodiment 1

[0044] Nanopolymer micelles loaded with antitumor drug paclitaxel (PTX) and superparamagnetic iron oxide nanoparticles (SPIO) were prepared.

[0045] Encapsulation of SPIO nanoparticles and loading of hydrophobic antitumor drug paclitaxel by self-assembly of amphiphilic block polymer micelles. 0.6mg of PTX, Fe 3 o 4 6.0mg of nanoparticles, 13.5mg of MPEG-PCL and 3mg of FA-PEG-PCL, 3mg of FITC-PEG-PCL were dissolved in 1.5ml of tetrahydrofuran (THF), and then the above mixture was added to 15ml of deionized water under ultrasonication, and dialyzed for 2 THF was volatilized for 3 days to remove organic solvents and unloaded anti-tumor drugs, and the obtained nanopolymer micelles of FA-NP / SPIO / PTX were filtered through a 450nm filter membrane and ultrafiltration centrifuge tubes to obtain Centrifuge twice at 2500-3000rpm.

[0046] Measurement of the particle size of the nanopolymer micelles of FA-NP / SPIO / PTX prepared in Example 1.

[0047] Using dynamic light scattering to m...

Embodiment 2

[0062] Nanopolymer micelles loaded with antitumor drug paclitaxel (PTX) and superparamagnetic iron oxide nanoparticles (SPIO) were prepared.

[0063] Encapsulation of SPIO nanoparticles and loading of hydrophobic antitumor drug paclitaxel by self-assembly of amphiphilic block polymer micelles. 0.1mg of PTX, 1.0mg of CoFe 3 o 4 Nanoparticles, 4mg MPEG-PCL and 0.8mgFA-PEG-PCL, 0.8mg FITC-PEG-PCL were dissolved in 2ml tetrahydrofuran (THF), and then the above mixture was added to 20ml deionized water under ultrasonication, and volatilized by dialysis for 3 days THF is used to remove organic solvents and unloaded anti-tumor drugs to obtain nanopolymer micelles of FA-NP / SPIO / PTX. The micellar solution is then filtered through a 450nm filter membrane and centrifuged twice at 3000rpm in an ultrafiltration centrifuge tube , to prepare the inner core loaded with hydrophobic drugs and magnetic resonance sensitive molecular probes, and the outer shell with active targeting groups to in...

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Abstract

The invention relates to a preparing method of a nanometer polymer micelle carrier integrating diagnosis and treatment. The method includes following steps of: (1) dissolving 0.1-1 part by weight of a hydrophobic anticancer medicine, 1-10 parts by weight of a magnetic resonance sensitive molecular probe, 4-40 parts by weight of polyethylene glycol-polycaprolactone end-capped with methoxy, 0.8-8 parts by weight of a polymer micelle with an active targeting group and 0.8-8 parts by weight of FITC-PEG-PCL into 1-100 parts by weight of tetrahydrofuran to obtain a mixture; (2) adding the mixture prepared in the step (1) into 5-800 parts by weight of deionized water, dialyzing and volatilizing to remove the organic solvent to obtain a targeted FA-NP / SPIO / PTX nanometer polymer micelle; and (3) subjecting the targeted FA-NP / SPIO / PTX nanometer polymer micelle prepared in the step (2) to filter membrane filtration and centrifugation to remove the unloaded or uncovered antitumor medicine so as to prepare the nanometer polymer micelle carrier integrating diagnosis and treatment, wherein the inner core of carrier carries the hydrophobic medicine and the magnetic resonance sensitive molecular probe, and the outer shell of the carrier is provided with the active targeting group.

Description

technical field [0001] The invention relates to a preparation method of a nanometer polymer micelle carrier integrating diagnosis and treatment, in particular to a dual-function carrier with active targeting function and simultaneously loaded with hydrophobic antitumor drugs and magnetic resonance molecular imaging probes Preparation of nanopolymer micellar systems. This nano-polymer micelle system has broad application prospects in biomedical engineering fields such as drug release, early diagnosis of tumors, targeted molecular probes, magnetic resonance imaging tracking, and efficacy evaluation. Background technique [0002] About 6 million people die of malignant tumors in the world every year, and there are about 4.5 million cancer patients in China, with a mortality rate of about 30%, and it is increasing year by year. The non-surgical treatment of tumors includes radiotherapy and chemotherapy, both of which aim to kill tumor cells, but their biggest drawback is due to...

Claims

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Application Information

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IPC IPC(8): A61K49/18A61K49/12A61K45/00A61K9/107A61K47/34A61P35/00
Inventor 王巧英魏清荣鲁建
Owner 王巧英
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