Cadmium (Cd) strongly-induced promoter CdS2 and application thereof

Abstract

The invention discloses a segmented plant gene sequence. According to the segmented plant gene sequence, a cadmium (Cd) strongly-induced rice specific gene promoter CdS2 is separated, cloned and identified from Oryza sativa L cv.Nipponbare, and acquired transgenic rice is subjected to cadmium (Cd) treatment and is subjected to GUS chemical dyeing to find that each part of treated transgenic plants is significantly dyed; and moreover, by analyzing Gus gene expression quantities before and after RT-qPCR identification treatment, results show that the Gus gene expression quantity of the transgenic plants after being subjected to cadmium (Cd) treatment is 887.9 times that of the transgenic plants before being subjected to cadmium (Cd) treatment and can reach 4 times of ACTIN activity of a constitutive promoter. The promoter disclosed by the invention can be used for reflecting the cadmium pollution strength in soil according to the expression quantity of the promoter in rice, has the characteristics of simplicity in operation, low detection limit, micro sensitivity and high accuracy, can play important significance in researching environment relationships, improving ecological environments and achieving sustainable development, can also be used for actively analyzing pollution formation principles, and ensures that valuable experiences for avoidance and controllable transformation of cadmium pollution can be further provided.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant cadmium-inducible expression promoter and its expression and application in rice. The promoter can drive the expression of a target gene in a plant under cadmium-induced conditions in a rice transgenic regulation system, and It can be used to determine the cadmium content in soil. Background technique [0002] With the rapid development of modern industrial and agricultural production, the industrial "three wastes", the amount of agricultural chemical fertilizers, and solid waste continue to increase, resulting in a sharp increase in the content of heavy metals in the soil. The problem of heavy metal pollution is becoming more and more serious, and has become one of the main problems worldwide (Deng Xiangxiong et al., 2009). Cadmium (Cd) is one of the most toxic heavy metals. Compared with other non-esse...

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Problems solved by technology

The national standard analysis method is the atomic absorption method. Although the detection limit of this method is low, there are many coexisting ions after the soil pollution is decontaminated, which will interfere with the determination of cadmium to a certain extent, and the metal ion has the greatest interference effect.

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