A bacterial agent for rapid start-up and efficient operation of anaerobic digestion of food waste

An anaerobic digestion, quick-start technology, applied in biochemical equipment and methods, bacteria, microorganism-based methods, etc., can solve the problems of differences in the composition of kitchen waste, non-contained, and unable to adapt to kitchen raw materials, etc. Start-up time, effect of efficient anaerobic digestion

Active Publication Date: 2018-04-27
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition and properties of food waste are very different, and only relying on the above five kinds of microorganisms cannot adapt to all types of food raw materials.
[0008] Although the above-mentioned patents can improve the anaerobic digestion of food waste to varying degrees, none of them includes the content of accelerating the start-up process of anaerobic digestion of food waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The experiment was carried out using a two-phase anaerobic digestion process, and the specific steps are as follows:

[0022] 1) Preparation of biogas-producing bacteria

[0023] The bacterial agent includes the following strains: 17 parts of Clostridium japonica DSM19732, 120 parts of Saccharophilus Saccharophilus DSM22681, 85 parts of Thermus faecalilyticus DSM5265, 23 parts of Thermoanaerobacter DSM14523, 16 parts of fast-growing Thermomycobacterium DSM8682, 11 parts of Thermobacterium shore DSM21630, 127 parts of Syntrophomonas DSM4212, 470 parts of thermoautotrophic Methanosarcina DSM1053, 190 parts of Methanosarcina thermophila DSM1825, 166 parts of Marseille Lumini Methanococcus DSM25720, 19 copies of Methanobacillus formate DSM3637. The concentration of each strain is 1×10 8 Individual / ml, the above strains were mixed.

[0024] 2) Construction of acidification tank

[0025] Acidification tank volume 6L, effective volume 4L, constant temperature fermentation ...

Embodiment 2

[0031] The experiment was carried out using a two-phase anaerobic digestion process, and the specific steps are as follows:

[0032] 1) Preparation of biogas-producing bacteria

[0033] The bacterial agents used in the inoculum were prepared from the following strains: 18 parts of Clostridium jaundice DSM19732, 125 parts of Saccharophilus saccharophilus DSM22681, 89 parts of Thermobacter proteolyticum DSM5265, 24 parts of Thermoanaerobacter DSM14523, 17 parts Parts of fast-growing Thermus mycobacterium DSM8682, 12 parts of Thermobacterium shore DSM21630, 134 parts of Syntrophomonas DSM4212, 494 parts of thermoautotrophic Thermus methanosarcina DSM1053, 202 parts of thermophilic Methanosarcina DSM1825, 174 parts of Lumi Methanococcus nimasai DSM25720, 20 parts of Methanobacterium formici DSM3637; each part contains 3×10 8 individual / mgl; mix the above strains.

[0034] 2) Construction of acidification tank

[0035] Acidification tank volume 6L, effective volume 4L, constant ...

Embodiment 3

[0048] The experiment was carried out using a two-phase anaerobic digestion process, and the specific steps are as follows:

[0049] 1) Preparation of biogas-producing bacteria

[0050] The bacterial agent includes the following strains: 19 parts of Clostridium jaundice DSM19732, 130 parts of Stoolbacterium saccharophilus DSM22681, 93 parts of Thermobacter faecalilyticus DSM5265, 25 parts of thermophilic anaerobic Rope DSM14523, 18 parts of fast-growing Thermomycobacterium DSM8682, 13 parts of Thermobacterium shore DSM21630, 141 parts of Syntrophomonas DSM4212, 520 parts of Thermoautotrophic Methanosarcina DSM1053, 210 parts of Methanosarcina thermophila DSM1825, 182 parts of Marseille Lumini Methanococcus DSM25720, 21 copies of Methanobacterium formici DSM3637, each of which contains 4×10 8 Individual / ml; Mix the above strains.

[0051] 2) Construction of acidification tank

[0052] Acidification tank volume 400L, effective volume 320L, constant temperature fermentation at...

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PUM

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Abstract

The invention provides a microbial agent for quick start and efficient operation of anaerobic digestion of kitchen waste. The microbial agent comprises the following strains: bright yellow clostridium DSM19732, saccharophilous sewage bacillus DSM22681, proteolysis feces thermus DSM5265, thermophilic anaerolineae DSM14523, fast-growing thermobrachium DSM8682, riparian high temperature bacillus DSM21630, syntrophomonas DSM4212, methanothermobacter thermautotrophicus DSM1053, thermophilic methanosarcina DSM1825, Luminy Marseille methanococcus DSM25720 and methanobacterium formicium DSM3637. By using the microbial agent as inoculum, the quick start and efficient operation of anaerobic digestion of kitchen waste can be realized.

Description

technical field [0001] The invention belongs to the technical field of organic waste treatment, and in particular relates to a bacterial agent for rapid start-up and high-efficiency operation of anaerobic digestion of kitchen waste. technical background [0002] China produces a staggering amount of food waste every year. According to statistics, only in 2011, the national food waste output was more than 80 million tons (2012 China Statistical Yearbook), and it showed a rising trend year by year. Food waste is easy to rot and breed pathogenic bacteria, which will cause harm to the environment and public health. Therefore, a problem that cannot be ignored is how to dispose of these wastes scientifically and properly. On the other hand, food waste is rich in organic matter such as protein and starch, and is a good waste biomass raw material, which can be used as an ideal raw material for anaerobic digestion. "Turning waste into treasure" through anaerobic digestion can not o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P39/00C12P5/02C12R1/145C12R1/32C12R1/01
CPCC12N1/20C12P5/023C12P39/00Y02E50/30
Inventor 吴波李强尹小波张敏王星李政伟邓雅月代莉蓉周正
Owner BIOGAS SCI RES INST MIN OF AGRI
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