Microbial agent for quick start and efficient operation of anaerobic digestion of kitchen waste
An anaerobic digestion, quick-start technology, applied in biochemical equipment and methods, bacteria, microorganism-based methods, etc., can solve the problems of differences in the composition of kitchen waste, non-contained, and unable to adapt to kitchen raw materials, etc. Start-up time, effect of efficient anaerobic digestion
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Embodiment 1
[0021] The experiment was carried out using a two-phase anaerobic digestion process, and the specific steps are as follows:
[0022] 1) Preparation of biogas-producing bacteria
[0023] The bacterial agent includes the following strains: 17 parts of Clostridium japonica DSM19732, 120 parts of Saccharophilus Saccharophilus DSM22681, 85 parts of Thermus faecalilyticus DSM5265, 23 parts of Thermoanaerobacter DSM14523, 16 parts of fast-growing Thermomycobacterium DSM8682, 11 parts of Thermobacterium shore DSM21630, 127 parts of Syntrophomonas DSM4212, 470 parts of thermoautotrophic Methanosarcina DSM1053, 190 parts of Methanosarcina thermophila DSM1825, 166 parts of Marseille Lumini Methanococcus DSM25720, 19 copies of Methanobacillus formate DSM3637. The concentration of each strain is 1×10 8 Individual / ml, the above strains were mixed.
[0024] 2) Construction of acidification tank
[0025] Acidification tank volume 6L, effective volume 4L, constant temperature fermentation ...
Embodiment 2
[0031] The experiment was carried out using a two-phase anaerobic digestion process, and the specific steps are as follows:
[0032] 1) Preparation of biogas-producing bacteria
[0033] The bacterial agents used in the inoculum were prepared from the following strains: 18 parts of Clostridium jaundice DSM19732, 125 parts of Saccharophilus saccharophilus DSM22681, 89 parts of Thermobacter proteolyticum DSM5265, 24 parts of Thermoanaerobacter DSM14523, 17 parts Parts of fast-growing Thermus mycobacterium DSM8682, 12 parts of Thermobacterium shore DSM21630, 134 parts of Syntrophomonas DSM4212, 494 parts of thermoautotrophic Thermus methanosarcina DSM1053, 202 parts of thermophilic Methanosarcina DSM1825, 174 parts of Lumi Methanococcus nimasai DSM25720, 20 parts of Methanobacterium formici DSM3637; each part contains 3×10 8 individual / mgl; mix the above strains.
[0034] 2) Construction of acidification tank
[0035] Acidification tank volume 6L, effective volume 4L, constant ...
Embodiment 3
[0048] The experiment was carried out using a two-phase anaerobic digestion process, and the specific steps are as follows:
[0049] 1) Preparation of biogas-producing bacteria
[0050] The bacterial agent includes the following strains: 19 parts of Clostridium jaundice DSM19732, 130 parts of Stoolbacterium saccharophilus DSM22681, 93 parts of Thermobacter faecalilyticus DSM5265, 25 parts of thermophilic anaerobic Rope DSM14523, 18 parts of fast-growing Thermomycobacterium DSM8682, 13 parts of Thermobacterium shore DSM21630, 141 parts of Syntrophomonas DSM4212, 520 parts of Thermoautotrophic Methanosarcina DSM1053, 210 parts of Methanosarcina thermophila DSM1825, 182 parts of Marseille Lumini Methanococcus DSM25720, 21 copies of Methanobacterium formici DSM3637, each of which contains 4×10 8 Individual / ml; Mix the above strains.
[0051] 2) Construction of acidification tank
[0052] Acidification tank volume 400L, effective volume 320L, constant temperature fermentation at...
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