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A set of amnion cell separation, collection and purification

A technology of amnion cells and amnion, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of impure cell lines and inability to collect amnion cells efficiently, and increase digestion Efficiency, increased overflow difficulty, and difficult-to-operate effects

Active Publication Date: 2016-09-14
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the problem that the amnion often accumulates in the digestion container when the amnion cells are separated, resulting in a large number of cells remaining in the amnion tissue, the amnion cells cannot be collected efficiently, and the obtained cell line is not pure. It provides a method for separating amnion cells. Collect Purification Sets

Method used

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  • A set of amnion cell separation, collection and purification
  • A set of amnion cell separation, collection and purification
  • A set of amnion cell separation, collection and purification

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specific Embodiment approach 1

[0035] Specific embodiment 1: This embodiment is described in conjunction with the figure. The amnion cell separation, collection and purification kit of this embodiment includes amnion fixed rotating frame, amnion tissue digester 2, amnion cell separator 3, disposable screw filter 4, disposable screw filter A syringe 5 and a disposable screw centrifuge tube 6;

[0036] The amniotic fixed rotating frame includes a hollow storage tank 1-1, a front fixed frame 1-2, a rear fixed frame 1-3 and a thin plate 1-4, the top of the hollow storage tank 1-1 is provided with a threaded round rod 1-5, and a threaded round rod 1-5 is externally connected with T-shaped handle 1-6, the thin plate 1-4 is placed in the hollow storage tank 1-1, the size of the thin plate 1-4 matches the hollow storage tank 1-1, and the front of the hollow storage tank 1-1 The left side is hinged to one side of the front fixed frame 1-2, the front fixed frame 1-2 can be buckled around the hinge shaft and fixed on ...

specific Embodiment approach 2

[0042] Embodiment 2: This embodiment differs from Embodiment 1 in that: the digester inlet screw port 2-3 of the amniotic membrane tissue digester 2, the digester discharge screw port 2-4 and the digester cylindrical interface 2- 2 are provided with a cover; the separator inlet screw 3-3, the separator discharge screw 3-4 and the separator cylindrical interface 3-2 of the amnion cell separator 3 are all provided with a cover; disposable screw filter The liquid inlet connector 4-1 and the liquid outlet connector 4-2 of the device 4 are provided with threaded rubber plugs; the injection port of the disposable screw syringe 5 and the threaded interface of the disposable screw centrifuge tube 6 cover are provided with covers. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0043] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the threaded round rod 1-5 of the amniotic membrane fixed rotating frame 1 is an external thread, the T-shaped handle 1-6 is provided with an internal thread, and the amnion tissue digester The inlet screw 2-3 of the digester 2 is an internal thread, the drain screw 2-4 of the digester is an external thread, the cylindrical interface 2-2 of the digester is an external thread, and the separator of the amnion cell separator 3 enters the liquid The screw port 3-3 is an internal thread, the separator discharge screw port 3-4 is an external thread, and the separator cylindrical interface 3-2 is an external thread. Others are the same as in the first or second embodiment.

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Abstract

An amnion cell separation and collection purification set relates to an amnion cell separation set. The amnion cell separation and collection purification set is used for solving the problems that during amnion cell separation, an amnion is often accumulated in a digestion container, so that a lot of cells are remained among amnion tissues, the amnion cells cannot be efficiently collected, and the obtained cell line is unpurified. The set comprises an amnion cell fixing rotary frame, an amnion tissue digester, an amnion cell separator, a disposable screwed filter, a disposable screwed injector and a disposable screwed centrifuge tube; a threaded circular rod is arranged on the top of a hollow object placing groove of the amnion cell fixing rotary frame; the threaded circular rod is externally connected with a T-shaped handle; a thin plate is arranged in the object placing groove; the amnion tissue digester is of a hollow cube structure; a top cover is arranged on the top of the amnion tissue digester; a liquid inlet screwed opening is formed in one side of the upper part of the amnion tissue digester; a liquid discharge screwed opening is formed in the bottom of the amnion tissue digester; the amnion cell separator is of a hollow cylinder structure; a top cover is arranged on the top of the amnion cell separator; a liquid inlet screwed opening is formed in the side face of the upper part of the amnion cell separator; a liquid discharging screwed opening is formed in the bottom of the amnion cell separator. The amnion cell separation and collection purification set is used for separating and collecting amniotic epithelial cells.

Description

technical field [0001] The invention relates to an amnion cell separation set. Background technique [0002] Placental villi and amniotic membranes are the wastes of pregnant women after childbirth. They have a wide range of sources, are easy to obtain, and have no ethical and moral disputes. They have become research hotspots in recent years. The amniotic membrane on the placenta is a translucent membrane, and there are no blood vessels, nerves, muscles and lymphoid tissues on its surface, and amniotic epithelial cells and amniotic mesenchymal stem cells can be isolated from it. [0003] Amniotic mesenchymal stem cells come from the extraembryonic mesoderm in the primitive streak stage. Amniotic mesenchymal stem cells have a wide range of differentiation capabilities and can differentiate into a variety of mature somatic cells under certain conditions, such as osteoblasts, adipocytes, and liver cells. cells, nerve cells, and cardiovascular endothelial cells. Moreover, by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/06
Inventor 张怡付寅生刘艳青
Owner 天晴干细胞股份有限公司
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