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Fu tea and preparation method and use thereof

A kind of technology of Fu tea and its use, which is applied in the field of Fu tea and its preparation, can solve the problems that need to be deepened, and achieve the effects of improving lipid metabolism, stable quality, and lowering fasting blood sugar

Inactive Publication Date: 2015-07-01
BGI SHENZHEN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current research on regulating the gut microbiota in animals still needs to be in-depth

Method used

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  • Fu tea and preparation method and use thereof
  • Fu tea and preparation method and use thereof
  • Fu tea and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: the preparation of fu tea

[0040] 1. Preparation of spore suspension of S. coronis

[0041] Use a sterile inoculation needle to pick out the yellow obturator shells on Fuzhuan brick tea cubes and inoculate them on Chapei's medium, and cultivate them at 28°C. When cultured for 72 hours, pick a small amount of yellow closed cysts and separate them by streaking on a plate. After 2 to 3 streaks on a plate, the bacteria has been preliminarily purified. When the initially purified bacterial colony is in the vigorous growth period, use the inoculation loop to scrape the yellow occiput shell into a sterile vial filled with 10ml of sterile water, shake for 20min to make a uniform spore suspension and increase it by 10 times diluted to make 10 -3 ~10 -5 Diluted spore suspension.

[0042] Use a sterile pipette to draw 0.1ml of the spore suspension of each dilution and spread it on the blank Chapeau medium, and then culture it at 28°C. Select a plate with only 1...

Embodiment 2

[0050] Example 2: Function experiment of Fu tea regulating human intestinal flora

[0051] According to the Ministry of Health "Technical Specifications for Testing and Evaluation of Health Drinks (2003)", the method for evaluating the function of intestinal flora in human body is tested and evaluated for the Fucha prepared in Example 1, as follows:

[0052] 1. Drinking experiment group

[0053] Table 1 Grouping table of drinking experiment

[0054]

[0055] 2. Human body drinking test method

[0056] The subjects were randomly divided into two groups, 20 people in each group. Before the subjects tried to drink, the feces of the subjects were collected aseptically, and the intestinal flora was tested by 16S rDNA sequencing as a reference for the background level.

[0057] According to the grouping situation in Table 1, group 1 took the control group and group 2 took the experimental group. After 4 weeks, the feces of the subjects were collected, and 16SrDNA sequencing was...

Embodiment 3

[0066] Embodiment 3: Animal experiment of Fu tea in reducing blood sugar

[0067] The Fu tea prepared in Example 1 was evaluated according to the animal experiment evaluation method for hypoglycemic function of the Ministry of Health "Technical Standards for Inspection and Evaluation of Health Drinks (2003)", specifically as follows:

[0068] 1. Test method: 30 male SD rats were taken, and a low-dose streptozotocin-induced diabetic rat model was prepared according to the standard method. Three days later, the rats were randomly divided into 3 groups according to the blood glucose level, 10 rats in each group. Each group was administered intragastrically for 8 days, once a day, and the blank group was given the same amount of distilled water. Two hours after the last administration, blood was taken to measure the blood sugar level.

[0069] 2. Test object:

[0070] Blank group: given distilled water;

[0071] Control group 1: Metformin 300mg / kg was given;

[0072] Experiment...

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Abstract

The invention discloses Fu tea and a preparation method and use thereof. The method comprises the following steps: providing a eurotium cristatum spore suspension; and inoculating the eurotium cristatum spore suspension in raw dark green tea and fermenting to obtain the Fu tea. By virtue of the method, the Fu tea with flourishing golden flowers can be effectively prepared, and the method is simple and is easy to operate and control.

Description

technical field [0001] The present invention relates to Fucha and its preparation method and application, in particular to a method for preparing Fucha, Fucha and the use of Fucha in regulating intestinal flora of animals. Background technique [0002] The human gastrointestinal tract inhabits about 10 4 An order of magnitude of normal flora, the total amount of which is similar to that of the liver. Gut microbes contain a wide variety of enzyme systems, which can participate in a series of physiological processes such as host energy, material and genetic information transfer. Many studies have proved that intestinal microorganisms can form a biological barrier in the intestinal tract, and inhibit the excessive growth of opportunistic pathogenic bacteria and foreign pathogenic bacteria through space occupation effect, nutrient competition and various metabolites and bacteriocins secreted invasion. More and more evidence shows that the dysregulated intestinal flora plays a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/34
Inventor 丁淑娟张海峰过敏朱江阳朱端清张珊珊朱倩张亮波刘天翔张耕耘金桃
Owner BGI SHENZHEN CO LTD
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