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Phoebe bournei tissue culture and rapid propagation method

A technology of the Minnan group and Minnan, applied in the field of plant tissue culture

Inactive Publication Date: 2015-06-24
罗焕荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no foreign scholars' research reports on Minnan, and there are some scattered researches on seed germination and biomass in China, and there are few reports on its asexual reproduction technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Induction culture: collect the young shoots from the roots of the excellent plants of Minnan, and cut off the required stem segments as explants. Soak the explants in detergent for 2 hours, and at the same time brush the soft hairs on the surface, then rinse them with tap water for 4 hours, cut the stems into 1.0-1.5cm stem segments with 1-2 axillary buds. On the ultra-clean workbench, sterilize with 70% ethanol solution for 10 seconds, soak in 0.1% mercury liter for 12 minutes, wash with sterile water 7 times, inoculate into induction medium for adventitious bud induction. After inoculation, it is placed in the light for 13 hours a day, the light intensity is 2000lx, and the culture temperature is 25°C to cultivate until adventitious buds are formed, the induction rate is 67.8%, and the induction medium is: MS+5.5mg / L 6-BA+1.5mg / L NAA+23g / L sucrose+4.1g / L agar, the pH is 5.6.

[0017] (2) Subculture: Cut off the shoots grown after step (1) from the base and tra...

Embodiment 2

[0021] (1) Induction culture: collect the young shoots from the roots of the excellent plants of Minnan, and cut off the required stem segments as explants. Soak the explants in detergent for 3 hours, and at the same time brush the soft hairs on the surface, then rinse them with tap water for 5 hours, cut the stems into 1.0-1.5cm stem segments with 1-2 axillary buds. On the ultra-clean workbench, sterilize with 70% ethanol solution for 20 seconds, soak in 0.1% mercury liter for 15 minutes, rinse with sterile water 7 times, inoculate into induction medium for adventitious bud induction. After inoculation, it is placed in the light for 14 hours a day, the light intensity is 3000lx, and the culture temperature is 26°C and cultivated until adventitious buds are formed, the induction rate is 70.1%, and the induction medium is: MS+6.0mg / L 6-BA+1.0mg / L NAA+28g / L sucrose+4.7g / L agar, the pH is 5.8.

[0022] (2) Subculture: cut the shoots grown after step (1) from the base and tran...

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Abstract

The invention discloses a phoebe bournei tissue culture and rapid propagation method. Phoebe bournei belongs to lauraceae machilus evergreen trees, is an endemic landscaping tree species and an excellent precious timber species in China, and is a valuable material applied to buildings, furniture, carving and precise wood patterns; due to various reasons, phoebe bournei is in an endangered state at present and is a second-class endangered tree species in China; and in order to promote the propagation of phoebe bournei, establishment of an in-vitro rapid propagation technology system of phoebe bournei is very necessary. According to the phoebe bournei tissue culture and rapid propagation method disclosed by the invention, stems with buds are taken as explants, a phoebe bournei in-vitro regeneration plant can be successfully obtained by virtue of processes such as induced culture, subculture, rooting and acclimatization and transplanting, and the in-vitro rapid propagation technology system of phoebe bournei can be established so as to ensure that the phoebe bournei tissue culture and rapid propagation method has important realistic significance in rapid propagation and large-scale production and development of improved varieties of phoebe bournei.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Phoebe nannuum. Background technique [0002] Fujian Nan ( Phoebe bournei ) is an evergreen tree of the lauraceae genus Phoebe. It is a unique landscaping tree species and an excellent precious timber tree species in my country. Its wood cutting surface is smooth and beautiful, and it is a precious material for construction, furniture, carving and precision wood models. Among the ancient buildings, such as the world natural and cultural protection heritage - the Hokkien boat coffin in the Baiyan cliff of Wuyishan Nature Reserve, which was built more than 3,000 years ago, is still well preserved and immortal; in the Ming Tombs of Beijing, there are The nanmu pillars embraced by the two are durable for a long time, which is enough to show the precious and immortal characteristics of nanmu. Howe...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G17/00
Inventor 罗焕荣
Owner 罗焕荣
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