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Grade classification method for evaluating in-vitro fertilization treatment embryo based on cleavage behaviors

A technology of grade classification and in vitro fertilization, which is applied in the direction of biochemical equipment and methods, measurement/testing of microorganisms, material inspection products, etc., and can solve the problems that morphodynamic indicators cannot be used universally

Inactive Publication Date: 2015-06-17
GUANGXIU-GAOXIN LIFE SCI CO LTD HUNAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has the following disadvantages: morphodynamic indicators are affected by many factors, such as culture system, patient age, egg maturation, insemination method, and ovulation stimulation program, etc.
Therefore, the morphodynamic index cannot be used in all laboratories

Method used

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  • Grade classification method for evaluating in-vitro fertilization treatment embryo based on cleavage behaviors

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Experimental program
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Effect test

Embodiment 1

[0033] This method is applied to the clinical evaluation of embryos of patients undergoing in vitro fertilization treatment. Embryos are observed in the process of in vitro culture using the conventional time-lapse imaging system built into the incubator. Set the shooting frequency to 5min / time and the exposure time to 70ms / time. On the first day after fertilization, all the embryos to be selected were transferred to a 3x3 / 4x4WOW petri dish prepared with G1.5 embryo medium for culture. Place the WOW petri dish containing embryos under the camera of the jet lag system, and use the cleavage behavioral embryo classification model (Figure 1) to evaluate the embryos on the fifth day after fertilization.

[0034] Among the 345 normally fertilized embryos, 96 of the first, second, and third cleavage have normal division behavior, which are rated as A-grade embryos; at least one of the first, second, and third cleavage occurs except for normal division behavior. There are 102 type I cle...

Embodiment 2

[0036] This method is applied to the selection of high-quality embryos for transplantation in patients undergoing in vitro fertilization treatment. The embryos are observed by jet lag in the process of in vitro culture. The embryo grade classification model of cleavage behavior (Figure 1) was used to evaluate and select embryos on the fifth day after fertilization. The enrolled patients were randomly divided into split behavior group and conventional morphology group. The environment of the two groups of incubators is 37℃, 6% CO 2 .

[0037] The split behavior group uses the method of the present invention, adopts the time difference observation system built in the incubator routinely used in clinical practice, and sets the shooting frequency to 5 min / time and the exposure time to 70 ms / time. On the first day after fertilization, all the embryos to be selected were transferred to a 3x3 / 4x4WOW petri dish prepared with G1.5 embryo medium for culture. Place the WOW petri dish cont...

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Abstract

The invention discloses a grade classification method for evaluating an in-vitro fertilization treatment embryo based on cleavage behaviors. According to the invention, on the premise that a human embryo cell is neither interfered nor damaged, data of dynamic observation on a human embryo is obtained by employing a time-lapse imaging technology and abnormal cleavage behaviors of the human cleavage-state embryo of in-vitro fertilization are collected and described in detail, and divided into 7 types. Based on the order of severity of abnormal cleavage, blastomere range affected by the abnormal cleavage behaviors, and the generation period of the abnormal cleavage behaviors, the qualitative embryo grade classification method with little subjectivity is established, for effectively screening out the cleavage-state embryo with maximum blastocyst development potential.

Description

Technical field [0001] The present invention relates to the evaluation of embryos of patients undergoing in vitro fertilization treatment in assisted reproductive technology and the selection of high-quality embryos for transplantation. Specifically, the embryos observed by jet-lag imaging technology during treatment are classified according to abnormal patterns of division behavior, and embryos are screened. The method of transplantation. Background technique [0002] In vitro fertilization-embryo transfer (IVF-ET) has become a routine treatment for infertility. Traditional methods often rely on the simultaneous transfer of multiple embryos to increase the pregnancy rate. However, multiple embryo transfers lead to an increase in the rate of multiple pregnancy and increase the risk of iatrogenic complications for the mother and fetus. The feasible method is to establish an effective embryo assessment Methods Select high-quality embryos for transfer during the cleavage stage. Th...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/68
Inventor 林戈卢光琇杨舒亭
Owner GUANGXIU-GAOXIN LIFE SCI CO LTD HUNAN
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