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A preparation method and application of lotus leaf cell secondary metabolite freeze-dried powder

A technology of secondary metabolites and freeze-dried powder, which is applied in the field of plant cell culture and cosmetics, can solve the problems of limited lotus leaf growth season, unfavorable health, and affecting the output of finished products, so as to reduce damage, shorten cell culture time, The effect of simplifying the production process

Active Publication Date: 2018-06-15
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical extraction method needs to go through five steps: pretreatment, initial extraction, macroporous adsorption resin separation, organic solvent purification, and post-treatment. The production process is complicated; at the same time, its safety is low, and a large amount of organic solvents are used in the production process, which is easy to remain in the finished product , is not conducive to health; it will cause environmental pollution, and a large amount of organic solvents will be discharged into the environment during the production process; limited by the growing season of lotus leaves, it will affect the output of finished products

Method used

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  • A preparation method and application of lotus leaf cell secondary metabolite freeze-dried powder
  • A preparation method and application of lotus leaf cell secondary metabolite freeze-dried powder
  • A preparation method and application of lotus leaf cell secondary metabolite freeze-dried powder

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Experimental program
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Effect test

Embodiment 1

[0031] 1. Obtain lotus leaf cell lines

[0032] 1) Take 1 kg of fresh lotus leaves for cleaning and disinfection, and soak in 10 L of 15% sodium hypochlorite for 20 minutes. Under aseptic conditions, pour out the lotus leaves gently, wash each time with 2 L of sterile deionized water, a total of three times, and gently stir during each washing process.

[0033] 2) Under sterile conditions, cut the sterilized lotus leaves into pieces with sterilized scissors, and the size of each piece is ≤1mm 2 .

[0034] Transfer the lotus leaf fragments to a sterile clean beaker, add 10ml MS (Murashige and Skoog) medium for each 1.0g fragment, first grade 0.05ml mass fraction of 0.2 cellulase and 0.05ml mass fraction of 0.2 pectinase, digest 3 hours.

[0035] 3) Filter the digestive juice with a 200-mesh sterile mesh sieve to remove impurities, extract 20ul lotus leaf cell filtrate, add it to the Countstar cell counter, centrifuge the filtrate for 10min under a centrifugal force of 250g, ...

Embodiment 2

[0065] 1. Obtain lotus leaf cell lines

[0066] 1) Take 1 kg of fresh lotus leaves for cleaning and disinfection, and soak in 10 L of 20% sodium hypochlorite for 20 minutes. Under aseptic conditions, pour out the lotus leaves gently, wash each time with 2 L of sterile deionized water, a total of three times, and gently stir during each washing process.

[0067] 2) Under sterile conditions, cut the sterilized lotus leaves into pieces with sterilized scissors, and the size of each piece is ≤1mm 2 .

[0068] Transfer the lotus leaf fragments to a sterile clean beaker, add 10ml of MS (Murashige and Skoog) medium per 1.0g of fragments, first grade 0.05ml mass fraction of 0.3% cellulase and 0.05ml mass fraction of 0.3% pectinase , digested for 3 hours.

[0069] 3) Filter the digestive juice with a 200-mesh sterile mesh sieve to remove impurities, extract 20ul lotus leaf cell filtrate, add it to the Countstar cell counter, centrifuge the filtrate under 250g centrifugal force for 1...

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Abstract

The invention discloses a preparation method and application of lotus leaf cell secondary metabolite freeze-dried powder. The preparation method specifically comprises the following steps: (1) acquiring a lotus leaf cell strain, namely, cooperatively digesting sterile lotus leaves by using cellulase and pectinase so as to obtain the lotus leaf cell strain; (2) culturing a great amount of lotus leaf cells, namely, 1) performing primary culture by using a #I sterilization culture medium, and 2) performing suspension culture by using a #II sterilization culture medium, so as to obtain a lotus leaf cell culture; (3) collecting the lotus leaf cell culture so as to prepare lotus leaf cell secondary metabolite freeze-dried powder, and applying the lotus leaf cell secondary metabolite freeze-dried powder to anti-aging cosmetics. By using a lotus leaf cell culture method and due to the cooperative application of cellulase and pectinase, secondary metabolite in the culture can be finally collected, and not only is the production process simplified, but also the lotus leaf cell culture efficiency and quality are improved; meanwhile the product is high in security and can be stably produced for a long time; the lotus leaf cell secondary metabolite freeze-dried powder has an anti-aging function when being used in cosmetics.

Description

technical field [0001] The invention belongs to the field of plant cell culture and cosmetics, and specifically relates to a plant cell culture method, which reduces the loss of cells by shredding instead of beating, and adopts pectinase and cellulase combined digestion to shorten the improvement of digestion time to obtain lotus leaves The secondary metabolites of cells are made into freeze-dried powder as anti-aging cosmetics. Background technique [0002] The lotus leaf is a plant lotus of the water lily family, also known as lotus leaf and lotus leaf. "Compendium of Materia Medica" records, "Lotus leaves make people thin and weak. Single consumption can eliminate yang and water floating qi. It can generate vitality, help the spleen and stomach, astringent essence and turbidity, loose blood addiction, reduce swelling and pain, and cause acne. The chemical components in the lotus leaf are mainly flavonoids and nisinine, both of which have high biological activity, and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K8/02C12N5/04A61Q19/10A61Q19/08A61Q19/00
Inventor 陈海佳王一飞葛啸虎吴子杰戴国胜
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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