Erythrocyte cryopreservation liquid and application thereof

Abstract

The invention discloses an erythrocyte cryopreservation liquid. The erythrocyte cryopreservation liquid comprises 0.8M of potassium citrate monohydrate, 0.4M of sodium dihydrogen phosphate dihydrate, 0.2M of disodium hydrogen phosphate dodecahydrate, and glycerin with the volume concentration of 20-60%. The erythrocyte cryopreservation liquid allows erythrocytes to be disposed at -20DEG C for a long term, and components added to the liquid can improve the water permeability of cell membranes, reduce the formation of intracellular ice crystals and effectively protect erythrocyte membranes; and a thawing protection liquid is added when erythrocytes are thawed at room temperature, so the internal and external physiologic balance of erythrocyte membranes is realized, the rupture of the erythrocyte membranes is avoided, and the purpose of high recovery rate of the erythrocytes is reached.

Description

technical field [0001] The invention belongs to the technical field of red blood cell cryopreservation, and in particular relates to a red blood cell cryopreservation solution and an application thereof. Background technique [0002] In clinical blood transfusion work, the most important thing is to transfuse red blood cell products, the purpose of which is to improve the oxygen supply status of clinical patients. In clinical blood transfusion practice, it is found that if there is an antibody against the red blood cell antigen of the other party in the serum of the blood recipient or blood donor, it may cause hemolytic blood transfusion adverse reactions, showing clinical symptoms of intravascular or extravascular hemolysis, threatening the blood recipient life safety. In order to ensure the safety of clinical blood transfusion, the red blood cell antigen and antibody of blood recipient and blood donor should be tested before blood transfusion to avoid the occurrence of he...

AI Technical Summary

Problems solved by technology

Freezing red blood cells is an effective technology for long-term preservation of red blood cells. Currently, the commonly used cryopreservation methods are -80°C or -196°C. In addition to the need for special cell preservation solutions, these methods also have the following disadvantages: ① Such methods require expensive equipment , The -80°C cryopreservation method requires a special ultra-low temperature refrigerator, and the -196°C cryopreservation method requires liquid nitrogen cryopreservation equipment, which is expensive for long-term operation; , the recovery rate is very low

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