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Establishing method for tissue culture and rapid propagation system for amorphophallus konjac

A technology of tissue culture and establishment method, which is applied in the field of plant tissue culture, can solve the problems of konjac industry development constraints, low natural reproduction rate, degradation of seed taro, etc., and achieve the effect of solving the shortage of seedlings and promoting development

Inactive Publication Date: 2015-06-10
陈凤佳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the cultivation area of ​​konjac has continued to expand, and the amount of seeds used has continued to increase. However, as a semi-wild plant, konjac has a very low natural reproduction rate, and the formation cycle of commercially valuable organs is long. and the lack of disease-resistant varieties and targeted drug control restrict the development of the konjac industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Callus induction: Select the vigorously growing flower konjac taro, wash it with water first, brush the vicinity of the main bud with a brush, dry it, and then cut the top core block with buds (2cm×2cm×2cm) , and scrape and wash its epidermis, put it in clean ceramic red, and cover a layer of gauze on the porcelain red, rinse it under tap water for 1 hour, first soak it in 75% ethanol for 5 seconds, and rinse it with sterile water for 3 times , 3 minutes each time, then disinfected with 0.1% mercuric chloride solution for 5 minutes, and then rinsed with sterilizing water 3 times, 3 minutes each time. Blot the water droplets on the surface of the taro with sterile filter paper, cut off the surface layer of 1mm, cut into small pieces of 0.5cm×0.5cm×0.5cm, soak them in 70mg / L citric acid for 2s, and inoculate them into the induction medium for healing. Injury tissue induction. After inoculation, culture in total darkness at 28°C for 7 days, then place in light for 12...

Embodiment 2

[0024](1) Callus induction: Select the vigorously growing flower konjac taro, wash it with water first, brush the vicinity of the main bud with a brush, dry it, and then cut the top core block with buds (2cm×2cm×2cm) , and scrape and wash its epidermis, put it in clean ceramic red, and cover a layer of gauze on the porcelain red, rinse it under tap water for 2 hours, first soak it in 75% ethanol for 7 seconds, and rinse it with sterile water for 5 times , 5 minutes each time, then disinfected with 0.1% mercuric chloride solution for 6 minutes, and then rinsed with sterilizing water 4 times, 3 minutes each time. Blot the water drops on the surface of taro with sterile filter paper, cut off the surface layer 2mm, cut into small pieces of 0.5cm×0.5cm×0.5cm, soak them in 100mg / L citric acid for 3s, and inoculate them into the induction medium for healing. Injury tissue induction. After inoculation, culture in total darkness at 26°C for 9 days, then place in light for 14 hours a d...

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Abstract

The invention discloses an establishing method for a tissue culture and rapid propagation system for amorphophallus konjac, and relates to a method for quickly propagating and keeping original parent excellent-property seedlings within a short time by adopting a few tissues or organs as materials through the tissue culture technical means of the amorphophallus konjac. According to the establishing method disclosed by the invention, the underground tuber of the amorphophallus konjac is taken as an explant, and the tissue culture and rapid propagation of the amorphophallus konjac is realized through stages of callus induction, subculture, multi-shoot induction, test tube seedling rooting and test tube seedling acclimatizating and transplanting and the like, so that the problem that the seeds and seedlings for the amorphophallus konjac are deficient is solved, the amorphophallus konjac virus-free seedlings can be cultivated, purification and rejuvenation are performed on the variety, and the development of the amorphophallus konjac industry is promoted.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for establishing a fast propagation system for tissue culture of Amorphophallus konjac. Background technique [0002] Konjac ( Amorphophallus konjac ) is a perennial herbaceous plant belonging to the Araceae family Konjac. It is mainly distributed in Sichuan, Yunnan, Shaanxi, Ningxia, Gansu and other places in China, and the flower konjac is the main cultivated species and is the most widely distributed. It is distributed in all mountainous areas south of the Qinling Mountains and hilly terrain. The glucomannan rich in konjac bulbs has the characteristics of peptization, gelling, film-forming and excellent compounding properties with other plant gums, so it is widely used in food and health care, and has the functions of lowering blood fat and blood sugar. , anti-cancer, beauty, weight loss and other effects. [0003] In recent yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈凤佳
Owner 陈凤佳
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