Method for synthesizing vitamin A palmitate by using immobilized esterase E.coli BioH as catalyst

Abstract

The invention relates to a method for synthesizing vitamin A palmitate in a non-water-phase system by using immobilized esterase E.coli BioH as a catalyst. The method comprises the following steps: converting raw materials vitamin A acetate and lower alcohol into vitamin A alcohol by using immobilized esterase E.coli BioH as a catalyst, and carrying out esterification reaction with palmitic acid under non-water-phase conditions by using the immobilized esterase E.coli BioH as a catalyst to obtain the vitamin A palmitate. The conversion rate of the method is up to 98% above, the number of enzyme recycling times can reach more than 40, the subsequent product separation and purification process is simple, and the production cost is lower, thereby being beneficial to implementing industrialization. The vitamin A palmitate prepared by the method is light yellow grease on the appearance. The analysis by the United States Pharmacopeia USP28 process proves that the potency is 1.79-1.8 million IU / g, which the equivalent to the content of 98-99%; and the vitamin A palmitate can be widely used as a drug, feed additive, food additive or the like.

Description

technical field [0001] The invention relates to a method for synthesizing vitamin A palmitate, in particular to a method for catalyzing the synthesis of vitamin A palmitate by immobilized enzymes. Background technique [0002] Vitamin A palmitate has been widely used as medicine, food additive and feed additive, etc. There have been reports on various synthetic methods, including the preparation of vitamin A palmitic acid from vitamin A alcohol or lower fatty acid esters of vitamin A The ester method involves a variety of reaction routes. [0003] The synthetic route disclosed in Japanese Patent JP 62,248495 is: vitamin A acetate and palmitic acid are obtained under the action of lipase to obtain vitamin A palmitate; the synthetic route disclosed in British Patent GB 816224 is: vitamin A acetate and palmitic acid alkyl Vitamin A palmitate is obtained under the action of sodium methylate; the synthetic route disclosed in British Patent GB 1293041 is: Vitamin A alcohol and pa...

AI Technical Summary

Problems solved by technology

This method uses halogen raw materials and pyridine in the reaction process, the reaction conditions are milder than the acid chloride method, and the yield can reach 95%, but the product has a great influence on the thermal stability of the product due to the residue of halogen ions

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