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Growth factor-loading silk fibroin/collagen bracket material and preparation method thereof

A growth factor and collagen technology, applied in the field of silk fibroin/collagen scaffold material and its preparation, can solve the problems of losing biological activity and failing to reach the effective concentration, so as to prolong the functional effect and solve the half-life in vivo Too short, prolonged for full effect

Inactive Publication Date: 2015-06-03
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since TGF-β is a protein polypeptide, although it can promote the proliferation of chondrocytes and mesenchymal stem cells, it is sensitive to factors such as heat, pH, and enzymes. Without the protection of the carrier, it is easily degraded and loses biological activity and is blocked. Dilute without reaching the effective concentration

Method used

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  • Growth factor-loading silk fibroin/collagen bracket material and preparation method thereof
  • Growth factor-loading silk fibroin/collagen bracket material and preparation method thereof
  • Growth factor-loading silk fibroin/collagen bracket material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1: Preparation of loose layer silk fibroin / collagen film and dense layer silk fibroin / collagen film

[0026] 1. Preparation of silk fibroin

[0027] Cut silkworm cocoons into pieces and add 0.5% Na 2 CO 3 solution, in a constant temperature water bath at 98±2°C for 30 min, twice in total. After taking it out, wash it twice with boiling water, and then wash it 8 times with deionized water. Dry in an oven at 60° C. to obtain dry silk fibroin (ie, silk fibroin). Weigh the silk fibroin silk after silk degumming, use CaCl 2 / H 2 O / C 2 h 5 OH solution (molar ratio 1:8:2) was dissolved in a constant temperature water bath at (78±2)°C, and the bath ratio was 1:25. Filter with gauze to obtain the silk fibroin stock solution. Dialyze with a fibrous semipermeable membrane dialysis bag with a molecular weight cut-off of 8000 D-14000 D for 3 days to remove calcium chloride and ethanol. Filter to obtain silk fibroin solution.

[0028] 2. Collagen preparation

[...

Embodiment 2

[0035] Example 2: Preparation of TGF-β1 Nanoparticles Encapsulated

[0036] Polylysine is dissolved in deionized water with pH=7.4, the content is 1 mg.ml -1 . Dissolve TGF-β1 in 0.2 ML water, add 5 mL, 1 mg.ml -1 The sodium heparin solution was mixed overnight. Add TGF-β1-heparin sodium solution to 2 mL, 1 mg.ml -1 In the PLL solution, shake on a vortex mixer for 15 s, and then incubate at room temperature for 30 min. TGF-β1-PLL-heparin sodium nanoparticles can be obtained.

Embodiment 3

[0037] Example 3: Preparation of silk fibroin / collagen scaffold material loaded with TGF-β1

[0038] The cross-linked loose layer silk fibroin / collagen membrane prepared in Example 1 was wetted with double-distilled water and spread flat, and the PLL-heparin nanoparticle solution loaded with TGF-β1 prepared in Example 2 was added dropwise on the surface, 4- Air-dry at 8°C until there is no flowing liquid on the surface, then wet the cross-linked dense layer silk fibroin / collagen membrane and spread it on the upper surface of the cross-linked loose layer silk fibroin / collagen membrane, and air-dry at 4-8°C to obtain TGF-β1 silk fibroin / collagen scaffold material.

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Abstract

The invention discloses a growth factor-loading silk fibroin / collagen bracket material and a preparation method thereof. The material is prepared from the raw materials of collagens, growth factors, silk fibroin, polylysine (PLL) and heparin sodium. A loose layer fibroin / collagen film and a compact layer fibroin / collagen film form a double-layered structure, and growth factor-loading PLL-heparin sodium nanoparticles are compounded in the middle of the double-layered structure. The preparation method comprises the following steps: preparing the loose layer fibroin / collagen film and the compact layer fibroin / collagen film; preparing the growth factor-loading nanoparticles, compounding the fibroin / collagen film and the nanoparticles and the like. The growth factor-loading silk fibroin / collagen bracket material disclosed by the invention plays a directional differential slow release role on the loaded growth factors and can fully maintain the activity of the growth factors and effectively prolong the action time of the growth factors, so that the material is a good guided tissue regeneration material for prosthodontic treatment of impaired and damaged tissues.

Description

technical field [0001] The invention relates to a silk fibroin / collagen scaffold material loaded with growth factors and a preparation method thereof. Background technique [0002] Collagen is the structural protein of the extracellular matrix (ECM), found in humans and mammals. Collagen has the advantages of low antigenicity, can be absorbed by the human body, and has no toxic and side effects in vivo degradation products. It can promote cell adhesion and proliferation, maintain and promote the expression of cell phenotype, and is biodegradable. It is often used as a biomedical material. However, as a natural polymer, its performance cannot meet all the requirements. Silk is one of the earliest natural materials used by humans. As a fiber with excellent performance, it is mainly used in textiles. Recently, it is also used in many aspects such as biotechnology, medicine, and fine chemical industry, such as sutures after surgery, coatings, cosmetics, slow release of drugs, ...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/54
Inventor 张其清王建华程娘梅杨秋张志华
Owner FUZHOU UNIV
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