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Tissue culture and rapid propagation method for Cunninghamia lanceolate

A technology of tissue culture rapid propagation and Chinese fir, which is applied in the field of plant tissue culture, can solve the problems of poor seedling growth, unevenness, and high seedling cost, and achieve an effect that is conducive to large-scale production

Inactive Publication Date: 2015-06-03
朱海燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Chinese fir mainly adopts traditional methods such as cutting, grafting and sowing to raise seedlings. There are disadvantages such as high seedling cost, poor growth of seedlings, uneven growth, and failure to fully develop the potential of improved species, making it impossible to supply high-quality and strong Chinese fir seedlings. Meet the needs of the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Collection and disinfection of explants: take as explants plump shoots in the budding state at the base of fir stumps, rinse them under running water for 2 hours, soak in 3% washing powder solution for 5 minutes, and use a soft brush 3% washing powder solution to gently brush the material, then rinse with tap water in the form of dripping water for 60 minutes, rinse with distilled water for 3 times, soak in 75% ethanol solution for 20 seconds in an ultra-clean workbench, rinse with sterile water for 5 times, and then rinse with 0.01 %Tween-20 with 0.1% mercuric chloride solution for disinfection for 5 min, rinsed with sterile water for 3 times, and blotted the water on the surface with sterile filter paper for later use.

[0019] (2) Induction culture: cut the shoots treated in step (1) into stems of about 1.5 cm in length and inoculate them into induction medium for cluster bud induction culture. After inoculation, it was placed in the light for 12 hours a day, the...

Embodiment 2

[0024] (1) Collection and disinfection of explants: take as explants plump twigs in the budding state at the base of fir stumps, rinse them under running water for 3 hours, soak them in 3% washing powder solution for 8 minutes, and use a soft brush Gently brush the material with 3% washing powder solution, then rinse with tap water in the form of dripping water for 70 minutes, rinse with distilled water for 4 times, soak in 78% ethanol solution for 30 seconds in an ultra-clean workbench, rinse with sterile water for 5 times, and then rinse with 0.01 %Tween-20 with 0.1% mercuric chloride solution for 7 minutes, rinsed with sterile water for 5 times, and blotted the water on the surface with sterile filter paper for later use.

[0025] (2) Induction culture: cut the shoots treated in step (1) into stems of about 1.5 cm in length and inoculate them into induction medium for cluster bud induction culture. After inoculation, it was placed in the light for 13 hours a day, the light ...

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Abstract

The invention discloses a tissue culture and rapid propagation method for Cunninghamia lanceolate which is a main afforestation tree species specific to the southern China. At present, seedlings of the Cunninghamia lanceolate are mainly cultured with traditional methods such as cutting, grafting, seeding and the like, and defects that the seedling culture cost is high, seedlings grow poorly and are irregular, the potential of improved varieties is not fully played and the like exist. According to the method, excellent clone twigs are used as explants, in-vitro regenerated plants of the Cunninghamia lanceolate are obtained through processes of explant disinfection, induction culture, cluster bud propagation, adventitious root taking, acclimatization, transplantation and the like, a tissue culture and rapid propagation technology system for the Cunninghamia lanceolate is established, excellent characteristics of clone female parents of the Cunninghamia lanceolate can be kept, and mass production of excellent clone seedlings of the Cunninghamia lanceolate is facilitated.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of Chinese fir tissue culture. Background technique [0002] Chinese fir( Cunninghamia lanceolate ) is the main afforestation tree species endemic to southern my country. It grows fast, has soft texture, straight texture, easy processing, and many uses. It is one of the most important commercial materials in southern my country. In recent years, with the advancement of the reform of the collective forest right system and the rise in the price of commercial timber, forest farmers have become more active in managing Chinese fir, and the level of management and awareness of improved varieties of Chinese fir has been continuously improved. It is very necessary to multiply the fine genotypes of Chinese fir. [0003] In recent years, the rapid development of bioengineering technology, especially the establishment of ti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02P60/40Y02A40/22
Inventor 朱海燕
Owner 朱海燕
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