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COD (chemical oxygen demand) degradation microbial inoculant, and preparation method and application thereof

A technology for degrading bacteria and bacilli, which is applied in the direction of biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, can solve the problems of low concentration multiples, large water pollution, and high economic costs, so as to increase the concentration multiples and repeat Improvement of utilization rate and reduction of turbidity

Inactive Publication Date: 2015-05-20
CHONGQING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of low concentration multiples of traditional chemical agents, large water pollution and high economic costs, the present invention provides an environmentally friendly, low-cost COD-degrading bacterial agent that can greatly increase the concentration multiples of circulating water

Method used

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  • COD (chemical oxygen demand) degradation microbial inoculant, and preparation method and application thereof
  • COD (chemical oxygen demand) degradation microbial inoculant, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: COD degradation bacterium agent, its bacterium liquid volume ratio is:

[0035] Bacillus subtilis 20%;

[0036] Bacillus amyloliquefaciens 15%;

[0037] Anthrax Bacillus 10%;

[0038] Staphylococcus pasteurii 10%;

[0039] Pale green coccus 10%;

[0040] Lactobacillus casei 10%;

[0041] Spinosa lysinus 10%;

[0042] Lactococcus 15%.

[0043] Two, the preparation method of COD degrading bacterial agent

Embodiment 2

[0044] Embodiment 2: the preparation method of COD degrading bacterial agent, comprises the steps:

[0045] ①Strain preparation: Purchase Bacillus subtilis, Bacillus anthracis, Staphylococcus pasteurianus, Aureococcus aureus, Lactobacillus casei, Bacillus fusiformis, and Lactococcus, and activate the above-mentioned bacteria into original strains respectively kind;

[0046] ②Expanded cultivation: inoculate the original species of the activated strains in step ① into LB liquid medium for shaking culture, and ferment in batches in a fermenter. When the total number of microorganisms reaches 10 10 When the number / L is above, use storage tanks to collect the bacteria liquid of each strain, and put them in a 4°C environment for refrigerated storage for later use;

[0047] ③ Preparation of bacterial agent: prepare the bacterial solution of the above-mentioned bacterial species according to the following volume ratio:

[0048] Bacillus subtilis 20%;

[0049] Bacillus amyloliquefac...

Embodiment 3

[0057] Embodiment 3: the application of COD degrading bacterial agent, in the sump of circulating water system, drop into and account for the COD degrading bacterial agent of 0.1‰ of circulating water reserve, the volume ratio of each bacterial strain bacterial liquid in this COD degrading bacterial agent is as follows:

[0058] Bacillus subtilis 20%;

[0059] Bacillus amyloliquefaciens 15%;

[0060] Anthrax Bacillus 10%;

[0061] Staphylococcus pasteurii 10%;

[0062] Pale green coccus 10%;

[0063] Lactobacillus casei 10%;

[0064] Spinosa lysinus 10%;

[0065] Lactococcus 15%.

[0066] The test case of the application of this COD-degrading bacterial agent: Adding a COD-degrading bacterial agent with a water volume of 0.1‰ to a cooling water tank of a certain chemical circulating cooling water system, the results are as follows:

[0067] (1) Monitoring of water quality indicators

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Abstract

The invention discloses a COD (chemical oxygen demand) degradation microbial inoculant which comprises the following components in percentage by volume: 15-20% (preferably 20%) of Bacillus subtilis, 10-15% (preferably 15%) of Bacillus amyloliquefaciens, 10-20% (preferably 10%) of Bacillus anthracis, 10-15% (preferably 10%) of Staphylococcus pasteuri, 10-15% (preferably 10%) of Aerococcus viridans, 10-20% (preferably 10%) of Lactobacillus casei, 10-15% (preferably 10%) of Lysinibacillus fusiformis and 15-20% (preferably 15%) of Enterococcus. The COD degradation microbial inoculant effectively lowers the turbidity of the water body, and enhances the weight concentration of the water body on the premise of implementing environmental protection and saving water. The invention also discloses a preparation method and application of the COD degradation microbial inoculant.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of water, waste water or sewage. Background technique [0002] In the open circulating cooling water system, due to evaporation, the water in the circulation will be less and less, and the salt content in the water will be higher and higher. In order to maintain the salt content in the water at a certain concentration and the water level of the pool, fresh water must be added and concentrated water must be discharged. [0003] The concentration multiple is the ratio of the salt content in the circulating water to the salt content in the make-up water. However, in actual situations, due to salt deposition and other reasons, ions with relatively stable content and performance in the make-up water are generally used to calculate the concentration multiple. N=(a certain ion content in circulating water) / (a certain ion content in supplementary water), K is often selected for ions + , SiO...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/01C12R1/44C12R1/245
CPCC12N1/20C02F3/341
Inventor 叶姜瑜李大荣陈刚才窦建军
Owner CHONGQING UNIV
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