Extraction method of clerodendranthus spicatus

An extraction method and technology for kidney tea, which are applied in the field of extraction technology of plant natural elements, can solve the problems of loss of beneficial components of kidney tea, and achieve the effect of enhancing anti-inflammatory.

Inactive Publication Date: 2015-05-20
QINGDAO ZHONGREN ZHIYE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patents "Extraction method of kidney tea" (publication number CN 1723923A), "method of extracting compounds from kidney tea leaves and stems" (patent number ZL 200510127325.7) and "kidney tea anti-urinary calculus extract and its preparation method "(Publication No. CN 101066292A> discloses the technology that kidney tea is extracted with water, and alcohol is mentioned ethyl acetate is extracted, Chinese patent " a kind of kidney tea extract and preparation method and application " (publication number CN101143160A), but above-mentioned process The prepared kidney tea extract did not take into account the characteristics of kidney tea itself, and directly extracted with water, methanol, and ethyl acetate, resulting in the loss of many beneficial components in kidney tea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The present invention will be described in detail below in conjunction with embodiments.

[0015] 1. Washing and drying process: Take the raw materials of kidney tea, remove the sundries, wash with water, and dry in the sun.

[0016] 2. Crushing process: crush the dried kidney tea into 30-90 mesh particles.

[0017] 3. Pre-treatment process: add water and cellulosic mold to the crushed kidney tea particles, react at 25-30°C for 4 hours, then heat up to 60-70°C, react for 3 hours, cool to room temperature, and filter. Yes; the weight ratio of kidney tea to water is 1:5, and the weight / volume (kg / L) ratio of kidney tea and compound cellulase is 1:0.05.

[0018] The compound cellulosic mold is: a mixture of Trichoderma sp CGMCC 3.3068 and Trichoderma koningii CGMCC 3.2774, and the volume ratio of the expansion medium is 1:0.7.

[0019] 4. The post-treatment process is: extract the filtrate with ether for 5 times, combine the ether extracts, and evaporate to dryness at 40°C to obta...

Embodiment 2

[0021] 1. Washing and drying process: Take the raw materials of kidney tea, remove the sundries, wash with water, and dry in the sun.

[0022] 2. Crushing process: crush the dried kidney tea into 120-160 mesh particles.

[0023] 3. Pre-treatment process: add water and cellulosic mold to the crushed kidney tea particles, react at 30-35°C for 3 hours, then heat up to 65-77°C, react for 3 hours, cool to room temperature, filter, that is Yes; the weight ratio of kidney tea to water is 1:7, and the weight / volume (kg / L) ratio of kidney tea and complex cellulase is 1:0.5.

[0024] The compound cellulosic mold is: a mixture of Trichoderma sp CGMCC 3.3068 and Trichoderma koningii CGMCC 3.2774, and the volume ratio of the expansion medium is 1:0.6.

[0025] 4. The post-treatment process is as follows: extract the filtrate with ether 5 times, combine the ether extracts, and evaporate to dryness at 55°C to obtain a brown extract. The volume ratio of the filtrate to the ether is 1:0.6. The yiel...

Embodiment 3

[0027] Except that the kidney tea extract, which has been cooled to room temperature, was ultrasonically extracted for 30 minutes in ultrasonic waves with a frequency of 40,000 Hz, the rest was the same as in Example 1. The yield of the extract is 13% based on the raw material of kidney tea.

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PUM

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Abstract

The invention discloses an extraction method of clerodendranthus spicatus. The extraction method comprises the following steps: cleaning and drying, grinding and sieving, pretreatment, and post treatment. The pretreatment comprises the following steps: picking clerodendranthus spicatus particles with qualified sizes, adding water and composite cellulase into the clerodendranthus spicatus particles, carrying out reactions for 3 to 6 hours at a temperature of 25 to 35 DEG C, then heating to a temperature of 65 to 75 DEG C, keeping on reactions for another 3 to 6 hours, cooling to the room temperature, and finally filtering so as to obtain the clerodendranthus spicatus extract; wherein the weight ratio of clerodendranthus spicatus to water is 1:4-10, and the ratio of the clerodendranthus spicatus weight to composite cellulase volume (kg/L) is 1:0.05-0.5. Compared to the prior art, the cellulose in stems of clerodendranthus spicatus can be dissolved by the composite cellulase, so that the water-soluble beneficial components in the cellulose can be fully extracted, and the clerodendranthus spicatus functions of eliminating inflammation, dissolving stones, and promoting urination are enhanced.

Description

Technical field [0001] The invention belongs to the extraction process of plant natural elements, and particularly relates to an extraction method of kidney tea. Background technique [0002] Kidney tea is a plant of the genus Kidney tea in the Lamiaceae family, also known as cat's grass. There are about 5 species in the world. There is only one kind of kidney tea in my country, which is mainly produced in Guangxi, Guangdong, Yunnan, Fujian, Taiwan and other places. Folks believe that the whole herb has diuretic, antibacterial, anti-inflammatory, stone-dissolving and stone-eliminating effects. It is often used for acute and chronic nephritis, cystitis, urinary calculi, pharyngitis and rheumatoid arthritis. At present, 14 flavonoids have been separated from the above-ground part of kidney tea, 12 are flavonoids, 2 are flavonol glycosides; 8 phenolic acids, namely caffeic acid, the dimer of caffeic acid rosemary Acid, caffeoyl tartrate and dicaffeoyl tartrate, the condensate of ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P13/12A61P13/10A61P13/04A61P11/04A61P19/02A61P29/00
Inventor 谭斌峰
Owner QINGDAO ZHONGREN ZHIYE BIOTECH
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