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Extracting method and application for adenium obesum leaf cell extract

An extraction method and technology of rose leaves, which are applied in the field of extracting desert rose leaf cell extracts, can solve the problems of low extract content, low yield rate, and high impurity content, and achieve high yield, less impurities, and product safety. Effect

Inactive Publication Date: 2015-05-20
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned technology has the advantages of simple equipment, low cost, and non-toxic to the environment and humans, it has disadvantages such as low yield and high impurity content.
[0007] Due to the extremely low content of the extract obtained by the current extraction method, it is difficult to popularize the desert rose leaf cell extract, and to develop a high-yield extraction method has become a difficult problem that must be solved for the popularization and application of desert rose leaf cell extract

Method used

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  • Extracting method and application for adenium obesum leaf cell extract
  • Extracting method and application for adenium obesum leaf cell extract

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The extraction method of embodiment 1 desert rose leaf cell extract

[0030] A method for extracting desert rose leaf cell extracts, the specific steps are as follows:

[0031] (1) Select high-quality fresh, complete and impurity-free desert rose leaves, clean them and put them into a crushing device to crush them into raw material powders with a particle size of ≤40 mesh;

[0032] (2) Transport the raw material powder to the supercritical extraction kettle, and input CO into the supercritical extraction kettle. 2 Fluid, CO 2 The flow rate is 20-25kg / h, the extraction pressure is adjusted to 20-25MPa, and the extraction temperature is 35°C for extraction for 2 hours;

[0033] (3) Take out the extraction mixture from the outlet of the supercritical extraction kettle, input the mixture into the supercritical separation kettle, adjust the separation pressure to 8~12MPa, and the temperature is 40°C, and separate for 1 hour;

[0034] (4) Add the above separated product in...

Embodiment 2

[0035] Example 2 Application of desert rose leaf cell extract in skin care products

[0036] Add desert rose leaf cell extract to skin cream and other cosmetic production processes. This skin cream formula contains the following components: Stearic Acid 5%, Isopropyl Palmitate 4%, Span-602%, Lanolin 3%, Cetyl Alcohol 2%, Methylparaben 0.2%, Paraben Propyl ester 0.1%, monoglyceride 2%, white mineral oil 9%, essence 0.2%, desert rose leaf cell extract 4%, glycerin 4%, Tween-60 1.5%, DP-3000.1%, potassium hydroxide 0.2%, Allantoin 0.3%, water 62.4%.

Embodiment 3

[0037] The skin irritation test of embodiment 3 desert rose leaf cell extract

[0038] Add desert rose leaf cell extract to the production process of skin care cream and other beauty cosmetics, and conduct acute skin irritation test on white rabbits.

[0039] ①Choose three healthy white rabbits with similar individual size, and cut off the hair on both sides of their back spine by 2 cm x 2 cm.

[0040] ②Take 0.3 g of skin cream added with desert rose leaf cell extract and apply it directly on the skin on the right side of the white rabbit, cover it with clean gauze, and then fix it with non-irritating adhesive tape for 2 hours. The other side served as a negative control.

[0041] ③After removing the skin cream, observe the skin reaction of the white rabbit contacted parts and make corresponding records. The observation period is 1 hour, 6 hours, 12 hours, and 48 hours. The results are shown in Table 1.

[0042] Table 1: Acute skin irritation test results of desert rose leaf...

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Abstract

The invention discloses an extracting method for an adenium obesum leaf cell extract. The extracting method comprises the following steps: taking fresh adenium obesum leaves and crushing the fresh adenium obesum leaves; feeding raw material powder into a supercritical extraction kettle to perform supercritical CO2 extraction; taking and feeding an extracting mixture into a supercritical separation kettle to perform separation; adding separation products into distilled water to distill, and condensing and recycling steam to obtain the adenium obesum leaf cell extract. The extract is applied to a skin care product. Compared with the prior art, the extracting method can quickly extract much adenium obesum leaf cell extract on the premise of not damaging natural active ingredients of the adenium obesum, so that the yield is high, the impurities are few, solvent residues are avoided, and the natural characteristics of the extract are kept.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting desert rose leaf cell extracts and the application of the extracts in skin care products. Background technique [0002] Desert rose, also known as Tianbao flower, belongs to the family Apocynaceae, native to East Africa to the southern part of the Arabian Peninsula, and distributed in Tunisia, Algeria, Morocco and other North African countries. [0003] Desert rose likes high temperature, dry and sunny environment, it is resistant to intense heat, not cold, drought resistant, and avoids water temperature. Therefore, its planting environment is suitable for calcium-rich, well-drained sandy loam. The desert rose is shaped like a small trumpet, rose red, very gorgeous. The umbels are in clusters of three to five, bright and brocade, and bloom continuously in all seasons. It is named desert rose because its origin is close to the desert and it is a...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61Q19/08A61Q19/02A61Q19/00
Inventor 陈海佳王一飞葛啸虎曾维杰罗二梅
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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