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Quick nitrite detection method based on nanogold

A technology of nitrite and nano-gold, which is applied in the field of chemical analysis and detection, can solve problems such as heating, and achieve rapid and sensitive detection and convenient detection methods

Inactive Publication Date: 2015-05-13
HENAN INST OF ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has good selectivity for nitrite, it needs to heat the detection system to 95°C and keep it for 25 minutes to detect 1 mg / L nitrite
As another example, Xiao et al. (N. Xiao and C. X. Yu, Anal. Chem. , 2010, 82, 3659-3663.) reported a method based on p-mercaptoaniline-modified gold nanoparticles for the detection of nitrite, the detection limit of nitrite can reach 0.25 mg / L, and the detection time only needs 10 min , but the detection system still needs to react at 95°C

Method used

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  • Quick nitrite detection method based on nanogold
  • Quick nitrite detection method based on nanogold

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Gold nanoparticles sol preparation: 1 g HAuCl 4 4H 2 O was dissolved in 100 mL of water, and 1 mL of the above solution was diluted with 100 mL of water and transferred to a 250 mL clean three-neck flask, heated to boiling, and then 2 mL of 1% trisodium citrate dihydrate solution was added, and the Continue to heat and boil for 30 min while stirring, and cool down to room temperature naturally to obtain gold nano sol for later use.

[0029] Draw a standard curve for the detection of nitrite: take 10 serum bottles, add in turn to each serum bottle: 50 μL of 4-ATP solution with a concentration of 50 μmol / L, 10 μL of sulfuric acid with a concentration of 1 mol / L, 40 μL Different concentration of nitrite solution, get 100 μL solution A, so the concentration of 4-ATP in solution A is 25 μmol / L, the concentration of sulfuric acid in solution A is 0.1 mol / L, and in each serum bottle , the concentrations of nitrite in solution A were 0, 1, 5, 10, 15, 20, 25, 50, 100 and 200 μ...

Embodiment 2

[0033] Take 10 serum bottles, add 50 μL of 4-ATP solution with a concentration of 40 μmol / L, 20 μL of phosphoric acid with a concentration of 1 mol / L, and 40 μL of nitrite solutions with different concentrations to each serum bottle in turn, to obtain 100 μL of solution A, the concentration of 4-ATP in solution A is 20 μmol / L, the concentration of phosphoric acid in solution A is 0.2 mol / L, and in each serum bottle, the concentration of nitrite in solution A 0, 1, 5, 10, 15, 20, 25, 50, 100 and 200 μmol / L, respectively. Incubate solution A in the serum bottle at 10°C for 60 min to obtain solution B. Then add 0.4 mL of Na at a concentration of 0.16 mol / L to solution B 2 CO 3 -NaHCO 3 Buffer solution (pH=9.5), followed by adding 2 ml of the nano-gold sol prepared in Example 1, and after 20 min, the nano-gold sol was detected by visual observation or an ultraviolet-visible spectrophotometer. According to the measured UV-visible absorption spectrum data, the ratio of the absor...

Embodiment 3

[0036] Take 10 serum bottles, add 5 μL of 4-ATP solution with a concentration of 0.5 mmol / L, 5 μL of sulfuric acid with a concentration of 0.5 mol / L, and 90 μL of nitrite solutions with different concentrations to each serum bottle in turn, to obtain 100 μL of solution A, so the concentration of 4-ATP in solution A is 25 μmol / L, the concentration of sulfuric acid in solution A is 25 mmol / L, and in each serum bottle, the concentration of nitrite in solution A The concentrations were 0, 1, 5, 10, 15, 20, 25, 50, 100 and 200 μmol / L, respectively. Incubate solution A in the serum bottle at 25°C for 20 min to obtain solution B. Then add 0.4 mL of Na at a concentration of 0.1 mol / L to solution B 2 CO 3 -NaHCO 3 Buffer solution (pH=9.5), followed by adding 2 ml of the nano-gold sol prepared in Example 1, and after 15 min, the nano-gold sol was detected by an ultraviolet-visible spectrophotometer. According to the measured UV-visible absorption spectrum data, the ratio of the abso...

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Abstract

The invention discloses a quick nitrite detection method based on nanogold. The method comprises steps as follows: a standard solution containing nitrite is added to an acid aqueous solution of p-aminothiophenol, nanogold sol is added after a period of incubation, the nanogold sol is detected by an ultraviolet-visible spectrophotometer, ultraviolet-visible absorption spectrum data are acquired, the specific value of the absorbance in 685 nm and 520 nm positions is taken as the longitudinal coordinate, the concentration of the standard solution containing the nitrite is taken as the horizontal coordinate, a standard curve of the nitrite is obtained through fitting, the A685 / A520 value of a to-be-detected solution containing nitrite is introduced into a function relation of the standard curve of the nitrite, and quantitative detection of the nitrite is realized. The method is simple in step, low in cost, good in selectivity, high in detection speed and broad in application prospect, the nanogold is not required to be modified, special instrument equipment is not required, the detection range of the nitrite concentration is wide, and convenient, quick and sensitive detection can be realized.

Description

technical field [0001] The invention belongs to the field of chemical analysis and detection, and in particular relates to a technology for detecting nitrite by a colorimetric method based on nano-gold. Background technique [0002] Nitrite is ubiquitous in the natural environment, such as industrial waste water, acid rain and fertilizer degradation process will produce nitrite, in addition, nitrite is also used as an additive in meat products. However, excessive intake of nitrite can cause poisoning. The toxic effect is to paralyze the vasomotor center, respiratory center and peripheral blood vessels. In severe cases, blood pressure drops, coma, and death. Moreover, nitrite will produce nitrous acid in the stomach, and nitrous acid will react with protein decomposition products to easily produce nitrosamines, which have a strong carcinogenic effect. Therefore, it is very important to detect nitrite in the environment or food. [0003] At present, there are many methods fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 叶英杰郭怡肖海霞黄汉京赵龙涛
Owner HENAN INST OF ENG
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