Primary culture method of ampullaria gigas heart tissue cell

Abstract

The invention discloses a primary culture method of an ampullaria gigas heart tissue cell. The primary culture method comprises the following steps: preparing a cell culture solution; feeding a snail with the body weight of 40-50g in sterile water; sterilizing the surface of the ampullaria gigas; dissecting the ampullaria gigas to take a heart tissue; cleaning the tissue by using an HBSS cleaning fluid, and shearing into pieces; inoculating a cell culture bottle with the treated tissue; putting the culture bottle into an incubator with 5% CO2, and performing constant-temperature culture at 28 DEG C; staying overnight, adding a proper amount of the cell culture solution to immerse the tissue; sucking out the cell culture solution and replacing with an equal amount of the cell culture solution once every 5 days till the culture bottle is full of extended and proliferated cells. By the primary culture method, the primary culture of the ampullaria gigas heart tissue cell can be quickly established within a relatively short time in a repeatable manner; needed cell culture equipment is simple, and the operatability is strong; by the primary culture method, an important supplement to aquatic invertebrate cell culture is provided, and base data is provided for researching an immune mechanism of aquatic invertebrates.

Description

technical field [0001] The invention belongs to the technical field of freshwater biological cell culture, and in particular relates to a method for primary culture of apple snail core tissue cells. Background technique [0002] Since the in vitro culture method of animal tissues and cells was created by Harrison and Carrel at the beginning of last century, cell culture technology has been greatly developed, and has played an irreplaceable important role in both basic theoretical research and applied research. However, so far, the research on cell culture technology itself has mainly focused on vertebrates (especially mammals) and insects, and relatively little attention has been paid to the cell culture of aquatic invertebrates. Of the papers published in open journals on cell culture issues, the vast majority are of vertebrates and insects. [0003] Since the 1960s, researchers began to pay attention to the cell culture of aquatic invertebrates. Various tissues and cells ...

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Problems solved by technology

Later, due to the failure of marketization, apple snails were abandoned and spread rapidly, resulting in outbreaks and serious damage to crop production (Naylor, 1996)

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