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Device and method for quickly enriching Nx-DAMO bacteria

An anaerobic methane and nitrate nitrogen technology, applied in biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve the limitations of scientific research and technology development and application, limited biomass, and generation time Long and other problems, to achieve the effect of solving biomass loss, increasing microbial biomass, and large reaction rate

Inactive Publication Date: 2015-05-13
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the slow growth of Nx-DAMO microorganisms, the generation time is long, and the enrichment time is generally 8–16 months, and the enrichment stops after reaching a certain cell density, resulting in a limited biomass in the final harvest.
Difficulty in rapid mass enrichment culture is the bottleneck that limits the extensive scientific research and technology development and application in this field

Method used

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  • Device and method for quickly enriching Nx-DAMO bacteria
  • Device and method for quickly enriching Nx-DAMO bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Construction of a device for rapid enrichment of nitrate nitrogen reducing anaerobic methane oxidizing bacteria

[0030] 1. The device for rapidly enriching nitrate nitrogen-reducing anaerobic methane oxidizing bacteria, comprising a cylindrical glass reactor main body 1, an outer shell 2 is arranged outside the side wall of the reactor main body 1, the reactor main body 1 and the outer shell A sandwich cavity is formed between the two, and the outer casing 2 is provided with a water bath inlet 5 and a water bath outlet 6 communicating with the interlayer cavity, and the water bath inlet 5 and the water bath outlet 6 are relatively arranged on the outer casing 2 one by one; There are liquid outlet pipe 3, liquid inlet pipe 4 and reserved pipe 7, and the liquid outlet pipe 3, liquid inlet pipe 4 and reserved pipe 7 extend outside the outer casing 2; the top of the reactor main body 1 is provided with a top cover 8, and the reaction The upper end of the device ...

Embodiment 2

[0038] Embodiment two, sludge pretreatment and inoculation

[0039] 1. Mineral formula

[0040] Contain the following mineral components of following weight in every liter of mud-water reaction liquid (calculate by the mud-water mixed liquid volume of reacting after constant volume):

[0041] KHCO 3 : 1.0-1.5g; KH 2 PO 4 : 0.06g; CaCl 2 ·2H 2 O: 0.3g; MgSO 4 ·7H 2 O: 0.2g.

[0042] 2. Preparation of trace element solution

[0043] (1) Acidic trace element solution: prepared with distilled water, containing 2.1g FeSO per liter of solution 4 ·7H 2 O, 0.07g ZnSO 4 ·7H 2 O, 0.12g CoCl 2 ·6H 2 O, 0.5g MnCl 2 ·4H 2 O, 0.32g CuSO 4 , 0.1g NiCl 2 ·6H 2 O, 0.015g H 3 BO 3 , 100 mM HCl.

[0044] (2) Alkaline trace element solution: prepared with distilled water, containing 0.07g SeO per liter of solution 2 , 0.05g Na 2 WO 4 ·2H 2 O, 0.25g Na 2 MoO 4 , 10 mM NaOH.

[0045] 3. Sludge pretreatment and inoculation

[0046] Sludge pretreatment: Mix 60ml of lands...

Embodiment 3

[0048] Embodiment three, intermittent operation enrichment with NO 2 - n-DAMO bacteria as electron acceptors

[0049] 1. Build a batch reactor:

[0050] The reactor inoculated with the sludge in Example 2 was connected to the submersible pump in the constant temperature water bath via the rubber tube connected to the water bath inlet 5 and the water bath outlet 6 to form a constant temperature water bath circulation system. The electrode of the online pH analyzer is inserted into the electrode port 9 on the top of the reactor, and the sealing gasket and screw cap are put on to seal with the reactor to form an analysis system for real-time detection of pH and temperature. The liquid inlet pipe 4, the liquid outlet pipe 3, and the air inlet 11 are connected to rubber pipes and sealed with water-stop clips, and the gas sampling pipe 10 is sealed with a cover with a sampling pad. seal. Unused orifices were sealed with screw caps to ensure the closed system of the reactor. The...

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Abstract

The invention relates to a device for quickly enriching Nx-DAMO bacteria. The device comprises a reactor main body, wherein an outer housing is arranged outside the side wall of the reactor main body; an interlayer cavity is formed between the reactor main body and the outer housing; a water bath inlet and a water bath outlet, which are communicated with the interlayer cavity, are formed in the outer housing; a liquid outlet pipe, a liquid inlet pipe and a reserving pipe are arranged on the reactor main body; a top cover is arranged at the top end of the reactor main body; an electrode port and a gas production pipe are arranged at the upper end of the reactor main body; an exhaust branch pipe is arranged on the gas production pipe; a gas inlet pipe is arranged at the bottom of the reactor main body; an adsorption layer support frame is arranged in a circumferential direction in the middle of the inner side wall of the reactor main body; an adsorption layer is mounted on the adsorption layer support frame; an aeration plate support frame is arranged in the circumferential direction at the lower part of the inner wall of the reactor main body; and an aeration plate is mounted on the aeration plate support frame. The invention further relates to a method for quickly enriching Nx-DAMO bacteria with the device.

Description

technical field [0001] The invention belongs to the technical field of wastewater biological treatment, and relates to a device and method for rapidly enriching nitrate nitrogen reducing anaerobic methane oxidizing bacteria. Background technique [0002] The C and N cycles are the most basic geochemical cycles. The contribution of methane to global warming may reach 20%, which is second only to CO in the earth's atmosphere. 2 The greenhouse gas caused by the greenhouse effect is equimolar CO 2 20 to 30 times of that. The annual global methane production is about 300Tg, 90% of which is consumed before entering the atmosphere due to the anaerobic oxidation (AMO) of microorganisms, which slows down the emission of greenhouse gases. However, the emission of greenhouse gases during biological wastewater treatment has been widely concerned, except for N 2 In addition, recent studies have shown that 50% of the methane produced by the wastewater anaerobic treatment system is diss...

Claims

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Application Information

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IPC IPC(8): C12M1/04C12M1/02C12M1/00C12N1/20
Inventor 卢培利李微薇张代钧耿丹丹宋福忠张佩
Owner CHONGQING UNIV
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