Method for detecting types and contents of soil phenolic acids in eucalyptus forest land
A technique of eucalyptus forest and phenolic acid is applied in the field of detection of phenolic acid compounds, which can solve the problems of limited types of phenolic acids, decreased productivity, soil poisoning, etc., and achieves the effects of low detection cost, fast detection speed, and simple operation.
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[0019] The method for detecting phenolic acid species and content in eucalyptus woodland soil of the present invention comprises the following steps:
[0020] 1, seven kinds of mixed phenolic acid standard solutions preparation and standard curve drawing method, comprise the following steps:
[0021] (1) Accurately weigh 1, 5, 10, 50, and 100 mg of p-hydroxybenzoic acid, vanillic acid, ferulic acid, coumaric acid, benzoic acid, salicylic acid, and cinnamic acid, and dissolve the above mixture in 2 mL of methanol in a beaker, then dilute to 100mL with ultrapure water, and finally prepare the phenolic acid concentration gradients as 1, 5, 10, 50, and 100 μg·mL -1 mixed standard solution.
[0022] (2) Measured by high performance liquid chromatography waters-e2695, the detection wavelength is 230nm, the separation column is 4.00×250mm 5micron, and luna C is filled inside 18 (2), the column temperature is 30°C, the mobile phase is acetonitrile and 0.1% phosphoric acid solution, ...
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