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Method for detecting types and contents of soil phenolic acids in eucalyptus forest land

A technique of eucalyptus forest and phenolic acid is applied in the field of detection of phenolic acid compounds, which can solve the problems of limited types of phenolic acids, decreased productivity, soil poisoning, etc., and achieves the effects of low detection cost, fast detection speed, and simple operation.

Inactive Publication Date: 2015-05-06
GUANGXI UNIV
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Problems solved by technology

Some studies believe that the accumulation of phenolic acids in the soil is one of the important factors that hinder the continuous cropping of crops. With the increasing productivity decline of multi-generation continuous planting in artificial pure forests in my country in recent years, phenolic acids in forest soil It has also begun to attract the general attention of many scholars, who believe that phenolic substances may accumulate in the soil, thereby poisoning the soil, and become one of the important reasons for the continuous planting of plantations to reduce production. The report affects the seed germination and seedling growth of Chinese fir
The identification of fir phenolic acids uses supercritical extraction technology and gas chromatography-mass spectrometry. The extraction and identification process is relatively complicated, and the types of phenolic acids detected are limited.
In recent years, eucalyptus plantations have been widely planted in southern China, but the role of phenolic acid substances in eucalyptus woodland soil has been lack of research, and there are no related reports on the identification methods of phenolic acid substances in eucalyptus woodland soil

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  • Method for detecting types and contents of soil phenolic acids in eucalyptus forest land
  • Method for detecting types and contents of soil phenolic acids in eucalyptus forest land

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Embodiment 1

[0019] The method for detecting phenolic acid species and content in eucalyptus woodland soil of the present invention comprises the following steps:

[0020] 1, seven kinds of mixed phenolic acid standard solutions preparation and standard curve drawing method, comprise the following steps:

[0021] (1) Accurately weigh 1, 5, 10, 50, and 100 mg of p-hydroxybenzoic acid, vanillic acid, ferulic acid, coumaric acid, benzoic acid, salicylic acid, and cinnamic acid, and dissolve the above mixture in 2 mL of methanol in a beaker, then dilute to 100mL with ultrapure water, and finally prepare the phenolic acid concentration gradients as 1, 5, 10, 50, and 100 μg·mL -1 mixed standard solution.

[0022] (2) Measured by high performance liquid chromatography waters-e2695, the detection wavelength is 230nm, the separation column is 4.00×250mm 5micron, and luna C is filled inside 18 (2), the column temperature is 30°C, the mobile phase is acetonitrile and 0.1% phosphoric acid solution, ...

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Abstract

The invention relates to a method for detecting types and contents of soil phenolic acids in a eucalyptus forest land. The method comprises the following steps: sampling a soil sample in a forest land and bringing back to a laboratory for later use; preparing a phenolic acid mixed standard solution, injecting into efficient high performance liquid chromatography, determining proper wavelength and chromatographic conditions, carrying out linear regression according to phenolic acid concentration and peak area of a map to draw a standard curve; preparing a sample solution for test, and obtaining a sample solution by adding a NaOH solution, centrifugalizing, acidifying by virtue of hydrochloric acid, removing humic acid, filtering and the like; and feeding the sample by using an automatic sampler, measuring according to the chromatographic condition, recording the peak area, and calculating the types and contents of soil phenolic acids according to the standard curve of the phenolic acid mixed solution. The detection method provided by the invention is simple to operate, high in detection speed, high in sensitivity, strong in accuracy, good in repeatability and suitable for detecting the types and contents of p-hydroxybenzoic acid, vanillic acid, ferulic acid, cumaric acid, benzoic acid, salicylic acid and cinnamic acid in forest land soil.

Description

technical field [0001] The invention relates to a method for detecting phenolic acid compounds, in particular to a method for detecting the type and content of phenolic acid in eucalyptus woodland soil. Background technique [0002] In the life activities of plants, phenolic acids are one of the important secondary metabolites. Its biosynthesis is mainly through the shikimic acid pathway in the plant physiological process, and enters the soil through the secretion of plant roots, the leaching of the aboveground parts, the decomposition of litter and organic matter, and the activities of microorganisms, etc., affecting the nutrients in the soil. The effective form of substances and the distribution of microbial populations. Some studies believe that the accumulation of phenolic acids in the soil is one of the important factors that hinder the continuous cropping of crops. With the increasing productivity decline of multi-generation continuous planting in artificial pure fore...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 杨梅叶绍明廖承锐黄晓露谭玲赵毅辉易弘韬
Owner GUANGXI UNIV
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