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Biological enzyme-chemical compound catalyst and preparation method and application thereof

A composite catalyst and biological enzyme technology, which can be applied in the fields of biochemical equipment and methods, physical/chemical process catalysts, chemical instruments and methods, etc., can solve the problems that biological enzymes cannot be recycled, increase production costs, etc., and achieve good industrial application prospects. , mild reaction, no pollution to the environment

Inactive Publication Date: 2015-05-06
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of starch hydrolysis-hydrogenation one-pot production of sorbitol, the biological enzyme is in a free state, so the biological enzyme needs to be added every time it is reused, resulting in the biological enzyme cannot be recycled, which increases the production cost

Method used

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  • Biological enzyme-chemical compound catalyst and preparation method and application thereof
  • Biological enzyme-chemical compound catalyst and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1), preparation of chemical catalyst

[0031] Add 0.25g of mesoporous carbon spheres to 1.1mL of 0.06mol / L NH 4 RuCl 6 In aqueous solution, ultrasonic for 15 minutes, stand still for 12 hours, and dry at 100°C for 30 minutes. 0°C and under vigorous stirring, add 2.5 mL of 0.5 mol / L KBH dropwise to the above solution 4 Aqueous solution, stirring was continued for 30 minutes after the dropwise addition was completed. Centrifuge to obtain a black solid, which is repeatedly washed with deionized water until neutral.

[0032] Add the product obtained in the above steps into a mixed solution of 45mL of ethanol, 83mL of water, 0.17g of cetyltrimethylammonium bromide, 62μL of dodecanol and 1.7mL of 30mg / mL sodium hydroxide, and sonicate for 15 minutes. After stirring for 60 minutes, 2.7 mL of tetraethylorthosilicate and 0.2 mL of N-aminoethyl-γ-aminopropyltrimethoxysilane were slowly added dropwise. After stirring at 60°C for 3 hours, the product was washed by centrifugat...

Embodiment 2

[0040] (1), the preparation of chemical catalyst is the same as embodiment 1.

[0041] (2) A cross-linking reaction

[0042]Add the chemical catalyst with a core-shell structure obtained in step (1) and 50 μL of glutaraldehyde into 25 mL of water, sonicate for 15 minutes, stir for 15 minutes, centrifuge and wash three times, add 25 μL of glucoamylase, 25 mL of water, and stir in an ice-water bath for 4 hours. Centrifuge to obtain a black solid, which is repeatedly washed with deionized water until neutral.

[0043] (3) The secondary crosslinking reaction is the same as in Example 1, and the sample is marked as EI-Ru-B / mCairmSiO 2 -2.

Embodiment 3

[0045] (1), the preparation of chemical catalyst is the same as embodiment 1.

[0046] (2) A cross-linking reaction

[0047] Add the chemical catalyst with a core-shell structure obtained in step (1) and 150 μL of glutaraldehyde into 25 mL of water, sonicate for 15 minutes, stir for 15 minutes, centrifuge and wash three times, add 25 μL of glucoamylase, 25 mL of water, and stir in an ice-water bath for 4 hours. Centrifuge to obtain a black solid, which is repeatedly washed with deionized water until neutral.

[0048] (3) The secondary crosslinking reaction is the same as in Example 1, and the sample is marked as EI-Ru-B / mCairmSiO 2 -3.

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Abstract

The invention belongs to the technical field of catalysts and in particular relates to a biological enzyme-chemical compound catalyst, a preparation method of the catalyst, and an application of the catalyst in preparation of sorbitol. According to the catalyst, a spherical chemical catalyst is bonded to a dendritic biological enzyme catalyst and is of a core-shell structure, the shell structure refers to silica, and the core refers to mesoporous carbon spheres coated with ruthenium nanoparticles. A secondary cross-linked immobilization technology is adopted, the chemical catalyst is bonded by biological enzyme molecules by virtue of a cross-linking agent, the biological enzymes are mutually cross-linked to form a composite catalyst, the sorbitol is produced in an amylolysis-hydrogenation one-pot process and has excellent catalytic activity, the macromolecular biological enzyme is immobilized and can be recycled, the service life is long, the production cost is reduced, and the catalyst has good industrial application prospects. The catalyst disclosed by the invention is mild in reaction, readily available in raw materials, low in price and environment-friendly.

Description

technical field [0001] The invention belongs to the technical field of catalysts, and in particular relates to a biological enzyme-chemical composite catalyst, a preparation method thereof and an application in the preparation of sorbitol. Background technique [0002] Sorbitol is a very important raw material for the production of food, chemical, pharmaceutical and other industries. Its preparation mainly uses starch as raw material. Glucose is obtained by enzymatic hydrolysis of starch, and then the glucose is converted into sorbitol by metal-catalyzed hydrogenation. Its production is a two-step method, that is, the enzymatic hydrolysis and hydrogenation steps must be carried out separately, which not only causes the complexity of the operation process, but also increases the production cost. The main reason why the combination of metal catalysts and biological enzymes cannot be used directly for starch hydrolysis-hydrogenation one-pot production of sorbitol is that the me...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N9/34B01J23/46C12P7/18C07C31/26C07C29/141
CPCY02P20/50
Inventor 李辉魏巍赵羽王聪钟庆华李和兴
Owner SHANGHAI NORMAL UNIVERSITY
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