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Method for preparing nanoparticle dual-mode fluorescent probe by virtue of co-assembly technology

A technology of nanoparticles and fluorescent probes, applied in the field of preparation of nano-fluorescent probes, which can solve the problems of low biocompatibility, poor water solubility, and large size of composite nanoparticles

Inactive Publication Date: 2015-05-06
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the obtained composite nanoparticles are large in size, poor in water solubility, and low in biocompatibility.

Method used

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  • Method for preparing nanoparticle dual-mode fluorescent probe by virtue of co-assembly technology
  • Method for preparing nanoparticle dual-mode fluorescent probe by virtue of co-assembly technology
  • Method for preparing nanoparticle dual-mode fluorescent probe by virtue of co-assembly technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) The oleic acid (OA, Z-9-octadecenoic acid) ligand is coated with blue light (for the up-converted luminous color, it is only related to the type of rare earth metal doped, and has nothing to do with the size of the nanoparticles, etc. ) upconverting nanoparticles NaYF 4 : Yb, Tm (the molar ratio Y / Yb / Tm during preparation is 80 / 19 / 1; Wang.F., Liu.X.G.Chem.Soc.Rev., 2009,38,976-999) adding organic solvent chloroform Mix and centrifuge with anti-precipitant acetone (the volume ratio of anti-precipitant and organic solvent is 3-1:1), remove excess ligand, and redisperse the precipitate in chloroform at a concentration of 3 mg / mL;

[0025] 2) The orange-emitting (3.3nm) down-conversion nanoparticles CdTe coated with mercaptopropionic acid (MPA) (Zhang.H., Wang.L.P., Xiong.H.M., Hu.L.H., Yang.B.Adv.Mater .2003, 15, 1712-1715) after centrifugation (using a centrifuge at 9000-12000r / min, 10-20min), the precipitate was dispersed in water again.

[0026] 3) At room temperat...

Embodiment 2

[0028] 1) The oleic acid (OA, Z-9-octadecenoic acid) ligand is coated with blue light (for the up-converted luminous color, it is only related to the type of rare earth metal doped, and has nothing to do with the size of the nanoparticles, etc. ) upconverting nanoparticles NaYF 4 : Yb, Tm (the molar ratio Y / Yb / Tm during preparation is 80 / 19 / 1; Wang.F., Liu.X.G.Chem.Soc.Rev., 2009,38,976-999) adding organic solvent chloroform Mix and centrifuge with anti-precipitant acetone (the volume ratio of anti-precipitant and organic solvent is 3-1:1), remove excess ligand, and redisperse the precipitate in chloroform at a concentration of 3 mg / mL;

[0029] 2) Green-emitting (2.2nm) down-conversion nanoparticles CdTe coated with mercaptopropionic acid (MPA) (Zhang.H., Wang.L.P., Xiong.H.M., Hu.L.H., Yang.B.Adv.Mater .2003, 15, 1712-1715) after centrifugation (using a centrifuge at 9000-12000r / min, 10-20min), the precipitate was dispersed in water again.

[0030] 3) NaYF stabilized with ...

Embodiment 3

[0032] 1) The oleic acid (OA, Z-9-octadecenoic acid) ligand is coated with blue light (for the up-converted luminous color, it is only related to the type of rare earth metal doped, and has nothing to do with the size of the nanoparticles, etc. ) upconverting nanoparticles NaYF 4 : Yb, Tm (the molar ratio Y / Yb / Tm during preparation is 80 / 19 / 1; Wang.F., Liu.X.G.Chem.Soc.Rev., 2009,38,976-999) adding organic solvent chloroform Mix and centrifuge with anti-precipitant acetone (the volume ratio of anti-precipitant and organic solvent is 3-1:1), remove excess ligand, and redisperse the precipitate in chloroform at a concentration of 3 mg / mL;

[0033] 2) The down-conversion nanoparticles CdTe coated with mercaptopropionic acid (MPA) (Zhang.H., Wang.L.P., Xiong.H.M., Hu.L.H., Yang.B.Adv.Mater .2003, 15, 1712-1715) after centrifugation (using a centrifuge at 9000-12000r / min, 10-20min), the precipitate was dispersed in water again.

[0034] 3) NaYF stabilized with 3 mg / mL blue-emitti...

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Abstract

The invention relates to a method for preparing a nanoparticle dual-mode fluorescent probe by virtue of a co-assembly technology and belongs to the technical field of nano fluorescent probe preparation. The method comprises the following steps: firstly removing residual ligands from upconversion nanoparticles of an oil phase and supplementing a sulfydryl cadmium carboxylate complex to downconversion nanoparticles of the oil phase; mixing the upconversion nanoparticles of the oil phase and the downconversion nanoparticles of the oil phase and co-assembling two nanoparticles by absorbing upconversion nanoparticles of the oil phase by using the downconversion nanoparticles of the oil phase through continuous lucifugal stirring treatment. Co-assembly brings two results: one is that the upconversion nanoparticles are transferred from the oil phase to the water phase; the other one is that the dual-mode fluorescent probe is formed. The method provided by the invention is simple in integral operating process, less in dangerousness and high in yield of dual-mode fluorescent probe and has good experimental repeatability.

Description

technical field [0001] The invention belongs to the technical field of nano fluorescent probe preparation, and in particular relates to a method for preparing a water-soluble dual-mode nano fluorescent probe by co-assembling fluorescent nanoparticles in two phases of water and oil. In particular, it relates to a water-soluble down-conversion nanoparticle that uses oil-soluble up-conversion nanoparticles and mercaptocarboxylic acid as a ligand as a structural unit, and utilizes water-soluble down-conversion nanoparticles to absorb oil-soluble up-conversion nanoparticles. A method for transferring up-converting nanoparticles to the water phase and simultaneously forming dual-mode nano-fluorescent probes through treatment in the dark with stirring. Background technique [0002] As an important detection tool, fluorescent probes are widely used in molecular biology, cell biology, biochemistry, medicine, and chemical engineering. However, with the development of the biological f...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/85C09K11/88G01N21/64
Inventor 张皓李婷婷武振楠兰世杰杨柏
Owner JILIN UNIV
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