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Establishment method of fingerprint spectrum of traditional Chinese medicine composition

A technology of fingerprints and establishment methods, which is applied in the field of establishment of Kechuanshun pills and fingerprints, can solve the problems of few Kechuanshun pills and cannot fully reflect the effective chemical information of Kechuanshun pills, so as to ensure drug safety, The effect of guaranteeing the effect of treatment

Active Publication Date: 2015-04-29
GUANGZHOU BAIYUNSHAN CHENLIJI PHARMA FAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the material basis of Kechuanshun Pills, and the experimental results cannot fully reflect the effective chemical information in Kechuanshun Pills, so these research results cannot be used for quality control of Kechuanshun Pills

Method used

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  • Establishment method of fingerprint spectrum of traditional Chinese medicine composition
  • Establishment method of fingerprint spectrum of traditional Chinese medicine composition
  • Establishment method of fingerprint spectrum of traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1 Establishment of Chromatographic Conditions

[0086] Preparation of the test article:

[0087] Take 3g of the sample of batch number FT1641, pulverize it, accurately weigh it, place it in a 50ml Erlenmeyer flask, add 25ml of methanol precisely, ultrasonicate for 30min, let it cool to room temperature, and obtain it.

[0088] Chromatographic conditions:

[0089] Chromatographic instrument: Agilent 1200 high performance liquid chromatograph, chromatographic column: Agilent SB-C18 chromatographic column, column temperature 30°C, injection volume 10 μl, flow rate: 1ml / min.

[0090] 1.1 Determination of UV detection wavelength

[0091] The mobile phase is acetonitrile-0.1% phosphoric acid / water, using gradient elution, the elution time is 120 minutes, the volume percentage of acetonitrile is from 5% to 95%, and the volume percentage of 0.1% phosphoric acid solution is from 95% to 5%; respectively examine 225nm and 254nm , 283nm, 300nm, and 320nm wavelength ch...

Embodiment 2

[0112] Example 2 Examination of sample preparation methods

[0113] Under the chromatographic conditions established in Example 1, the chromatograms of the Kechuanshun Pill test solution prepared by ultrasonic extraction, reflux extraction, and cold extraction were investigated respectively.

[0114] Ultrasonic extraction: Take 3 g of Kechuanshun pills with batch number FT1641, pulverize them, accurately weigh them, place them in a 50ml Erlenmeyer flask, add 25ml of methanol precisely, and after ultrasonication for 30min, let cool to room temperature to obtain the obtained product.

[0115] Reflux extraction: Take 3g of Kechuanshun pills with batch number FT1641, pulverize them, accurately weigh them, put them in a 50ml Erlenmeyer flask, add 25ml of methanol precisely, heat to reflux for 2 hours, let cool to room temperature, and obtain the obtained product.

[0116] Cold soaking extraction: Take 3g of Kechuanshun pills with batch number FT1641, pulverize them, accurately we...

Embodiment 3

[0119] Example 3 Composition testing of Kechuanshun pills

[0120] 1. Chromatographic column: model is Agilent SB-C18 (5μm, 250×4.6mm ser#:880952-708);

[0121] 2. Mobile phase: use acetonitrile-0.1% phosphoric acid / water solution as the mobile phase, and carry out gradient elution according to the regulations in Table 2-4;

[0122] 3. Flow rate: 1.0mL / min;

[0123] 4. Detection wavelength: 225nm;

[0124] 5. The column temperature is 30°C;

[0125] 6. Preparation of the test solution: prepared according to the method described in the "ultrasonic extraction" item in Example 2;

[0126] 7. Determination method: Precisely draw 10 μl of the test solution, inject it into a liquid chromatograph, measure, and record the chromatogram for 120 minutes.

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Abstract

The invention provides an establishment method of fingerprint spectrum of a traditional Chinese medicine composition, and a method for detecting the constituents in the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following active ingredients: dried tangerine peels, radix peucedant, snakegourd seed, pinellia tuber, Radix Asteris, white mulberry root-bark, perilla fruit, bitter apricot seeds, common coltsfoot flowers, heartleaf houttuynia herb, poria and liquorice roots. The establishment method of the fingerprint spectrum comprises: detecting the retention times of compounds in the traditional Chinese medicine composition by adopting high-performance liquid chromatography.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for establishing a fingerprint of a traditional Chinese medicine composition. The traditional Chinese medicine composition is Kechuanshun Wan. Background technique [0002] Traditional Chinese medicine compositions (Chinese patent medicines) based on the theory of traditional Chinese medicine generally have a variety of medicinal flavor components; during the preparation process of the pharmaceutical composition, different components may react with each other, resulting in more complex components of the pharmaceutical composition. It is difficult to reflect the chemical characteristics of the composition as a whole due to the limited amount of information only by qualitative or quantitative detection of one or several components. With the development of modern analytical techniques, fingerprinting has become an internationally recognized quality control mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 蔡殷彭富全
Owner GUANGZHOU BAIYUNSHAN CHENLIJI PHARMA FAB CO LTD
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