Method for improving quality of chicken semen, primers, kit and method of use thereof

Abstract

The invention discloses a primer for a gene closely related to the quality of chicken semen. The primer is prepared from the following components including a mixture of upstream and downstream primers PCR amplified by a gene of a vasoactive intestinal peptide receptor 1 (VIPR1), a mixture of upstream and downstream primers PCR amplified by a gene of a vasoactive intestinal peptide receptor 2 (VIPR2) and a mixture of upstream and downstream primers PCR amplified by a gene of a dopamine receptor 2 (DAR2). A method of improving the quality of the chicken semen comprises the following steps: during breeding screening, extracting DNA of a chicken genome of a sample to be tested, carrying out specific primer amplification on a gene sequence to obtain target fragments of 192, 250 and 270 bp, after SSCP analysis and gel electrophoresis on the target fragments, coloring the target fragments by virtue of silver staining to obtain DNA and SSCP maps, and selecting a genotype individual of three genes of TT / GG / OQ according to banding pattern selection in three maps. A kit comprises mixtures of upstream and downstream primers PCR amplified by genes of the VIPR1, the VIPR2 and the DAR2 and a reagent needed by PCR. A using method of the kit comprises the following steps: carrying out PCR amplification on a fragment located by an SNP site; carrying out PCR-SSCP detection; and carrying out SSCP analysis. The method is remarkable in effect and simple and rapid in detection method and is not influenced by external environment.

Description

technical field [0001] The invention relates to a high-quality chicken semen quality closely related gene diagnosis kit and a use method thereof, which belong to the technical field of molecular biology and are applied to the early screening of roosters. Background technique [0002] Human beings have experienced thousands of years of unconscious and conscious selection of poultry production traits, but the process from phenotypic selection to genetic basis research and genotype selection is only a matter of recent decades. Excellent breeding roosters are one of the keys to improving economic benefits. On the one hand, they can reduce production costs, especially the breeding costs of breeding chickens. Adopting artificial insemination can increase the breeding ratio of chickens by about 3 times, and reduce 3 / 4 of the breeding cocks, which can greatly reduce the cost. On the other hand, it can greatly increase the utilization rate of excellent breeding cocks, improve product...

AI Technical Summary

Problems solved by technology

[0003] After searching the existing domestic and foreign literature and patents, there has been no report on the correlation between VIPR1, VIPR2 and DAR2 gene polymorphisms and chicken semen quality traits

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