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Early gastric adenocarcinoma primary cells single-stranded DNA aptamer and preparation method thereof

A technique for primary cells and gastric adenocarcinoma, which is applied in the field of pathogenic tissue aptamers and their preparation, can solve problems such as being unsuitable for screening early gastric adenocarcinoma, and achieves the effect of small molecular weight

Inactive Publication Date: 2015-04-29
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen that the single-stranded DNA aptamers prepared and disclosed at this stage in gastric adenocarcinoma primary cells are not suitable for screening early gastric adenocarcinoma

Method used

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  • Early gastric adenocarcinoma primary cells single-stranded DNA aptamer and preparation method thereof
  • Early gastric adenocarcinoma primary cells single-stranded DNA aptamer and preparation method thereof
  • Early gastric adenocarcinoma primary cells single-stranded DNA aptamer and preparation method thereof

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Embodiment Construction

[0024] The present invention is illustrated below in conjunction with examples.

[0025] The preparation method of the single-stranded DNA aptamer of primary gastric adenocarcinoma cells involved in the present invention is to obtain primary cells of early gastric adenocarcinoma and normal gastric mucosal epithelial cells through the screening of clinical patients, and then perform corresponding cloning processing, sequencing, etc. , and then perform specificity determination and affinity analysis on each aptamer obtained. Finally, the obtained aptamers were screened to obtain the desired aptamers.

[0026] In the present invention, primary cells of early gastric adenocarcinoma and normal gastric mucosal epithelial cells are obtained by separating and obtaining early gastric adenocarcinoma primary cells and normal gastric mucosal epithelial cells from the tissues of multiple patients undergoing radical surgery for early gastric cancer by the People's Hospital of Wuwei City and...

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Abstract

The invention discloses an early gastric adenocarcinoma primary cells single-stranded DNA aptamer and a preparation method thereof. The gene sequences of the early gastric adenocarcinoma primary cells single-stranded DNA aptamer are respectively: SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4 and SEQ ID No. 5. The early gastric adenocarcinoma primary cells single-stranded DNA aptamer can be applied in detecting early gastric adenocarcinoma, can be applied in preparing detection reagents for early gastric adenocarcinoma and can also be applied in preparing drugs for the treatment of gastric adenocarcinoma.

Description

technical field [0001] The invention relates to a disease-derived tissue aptamer and a preparation method thereof. Specifically, the present invention relates to a single-stranded DNA aptamer of early gastric adenocarcinoma primary cells and a preparation method thereof. Background technique [0002] Gastric cancer is one of the most common malignant tumors in the world and in my country, according to the global statistical report published by the World Health Organization [1] , there are 989,000 new cases of gastric cancer in the world, and the incidence rate is 17.6 / 100,000, ranking second only to lung cancer. Early gastric cancer is limited to the gastric mucosa and submucosa, regardless of whether there is lymph node metastasis. Because there are no obvious symptoms and local signs, it is often not enough for patients and doctors to pay enough attention to it, resulting in misdiagnosis or missed diagnosis. [0003] At present, the methods for preparing tumor cell singl...

Claims

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Application Information

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IPC IPC(8): C12N15/115C12N15/10C12Q1/68A61K48/00A61P35/00
Inventor 韩跃武徐攀邵明洋杜振亚张雪燕姚海燕马驰
Owner LANZHOU UNIVERSITY
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