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Application of 1-aminocyclopropane-1-carboxylic acid as bacterial chemotactic substance

A technology of aminocyclopropane and carboxylic acid, applied in the biological field, can solve problems such as inability to form bacterial film, and no longer have the effect of promoting production and increasing production.

Inactive Publication Date: 2015-04-29
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that once the ACC deaminase gene of many PGPR bacteria is knocked out, they will not be able to form a bacterial film on the root surface of the plant, and will no longer have the effect of promoting growth and production.
Whether PGPR bacteria can compete with other plant rhizosphere bacteria is because ACC produced by plant roots has a strong chemotaxis effect on PGPR bacteria, and whether ACC is a bacterial chemotactic substance has not been reported yet.

Method used

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  • Application of 1-aminocyclopropane-1-carboxylic acid as bacterial chemotactic substance
  • Application of 1-aminocyclopropane-1-carboxylic acid as bacterial chemotactic substance
  • Application of 1-aminocyclopropane-1-carboxylic acid as bacterial chemotactic substance

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Experimental program
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Embodiment 1

[0015] 1.1 Experimental strains

[0016] Pseudomonas putida UW4 ( Pseudomonas putida UW4): Deposited by the American Agricultural Research Culture Collection (the deposit number is NRRL B-50193, the deposit date is June 9, 2008). The United States Agricultural Research Culture Collection is located in Pecheria, Illinois. It is a government culture collection supported by the Agricultural Research Center of the United States Department of Agriculture. The full English name is Agrieultutal Research Service Culture Colleetion, or NRRL for short.

[0017] culture medium

[0018] LB liquid medium: 5 g yeast extract, 10 g tryptone, 10 g NaCl, 1000 mL deionized water.

[0019] LB solid medium: yeast extract 5 g, tryptone 10 g, NaCl 10 g, agar 20 g, deionized water 1000 mL.

[0020] TSB liquid medium: soy peptone 3 g, tryptone 17 g, glucose 2.5 g, sodium chloride 5 g, dipotassium hydrogen phosphate 2.5 g, add distilled water 1000 ml, pH 7.1-7.5, autoclave at 121 °C for 30 min....

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Abstract

The invention provides an application of ACC (1-aminocyclopropane-1-carboxylic acid) as a bacterial chemotactic substance. Through the plate chemotaxis assay, swam plate assay and capillary chemotaxis assay, a new use of ACC is found, namely, ACC has positive chemotaxis effect on bacteria, in particular, Pseudomonas putida, so that ACC can be used as the bacterial chemotactic substance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of 1-aminocyclopropane-1-carboxylic acid as a bacterial chemotactic substance. Background technique [0002] Bacterial movement tends to move towards certain chemical attractants (positive chemotaxis) or avoid certain harmful substances (negative chemotaxis), which is called bacterial chemotaxis. Substances that cause bacteria to produce positive chemotaxis are called positive chemotaxis substances, and conversely, substances that cause bacteria to produce negative chemotaxis are called negative chemotaxis substances. such as water hyacinth ( Eichhornia crassipes ) The amino acids methionine, glycine, alanine, and aspartic acid in the root exudate components have a significant effect on Enterobacter rhizosphere F2 ( Enterobacter sp. F2) has a strong positive chemotaxis effect and is a positive chemotaxis substance, while lysine, arginine, threonine, an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/40
CPCC12N1/20
Inventor 邱立友张朝辉黄涛高玉千戚元成申进文
Owner HENAN AGRICULTURAL UNIVERSITY
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