Special culture medium for liquefaction of Pleurotus eryngii and corresponding culture method
A strain culture medium and strain culture technology are applied in mushroom cultivation, cultivation, application and other directions, which can solve the problems that liquid fermented strains cannot be applied on a large scale, increase the risk of fruiting bag pollution, and produce fruiting bag pollution accidents. , to achieve the effect of eliminating secondary pollution, reducing the amount of inoculation, and shortening the cycle.
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Embodiment 1
[0038] Embodiment 1, a kind of pleurotus eryngii liquefaction special strain culture medium, it is made up of the composition of following parts by weight:
[0039]
[0040] The preparation method of above-mentioned culture medium is to carry out following steps successively:
[0041] In a stainless steel container, cornstarch, fine bran (wheat bran), and wheat cellulose are mixed uniformly under dry conditions to obtain ingredient I;
[0042] Add glucose, yeast extract, beef peptone, potassium dihydrogen phosphate, and magnesium sulfate into water, and fully stir (dissolve glucose, yeast extract, beef peptone, potassium dihydrogen phosphate, and magnesium sulfate in water) to obtain ingredient II;
[0043] After ingredients I and II are fully mixed, a culture medium specially used for the liquefaction of Pleurotus eryngii is obtained.
[0044] Remarks: The special culture medium for Pleurotus eryngii liquefaction is prepared and used now.
Embodiment 2
[0045] Embodiment 2, the Pleurotus eryngii liquefaction strain culture method that utilizes the substratum of embodiment 1 gained to carry out, the pleurotus eryngii female kind is carried out following steps successively:
[0046] 1), strain production:
[0047] 200g of the special strain culture medium for pleurotus eryngii liquefaction that has been prepared is immediately distributed in the special culture bottle of 200ml, and the microporous membrane ventilated cover is covered (selecting Shanghai Songtuo Industrial Co., Ltd. ZP14-200 ventilated culture bottle), in Carry out high temperature and high pressure sterilization at 121°C and 0.11Mpa for 90 minutes; obtain the sterilized medium;
[0048] Inject 5 grams of Pleurotus eryngii parent species (solid parent species) into the above-mentioned sterilized medium under sterile conditions, and culture in the dark at 22°C for 20 days to obtain solid strains dedicated to liquefaction; at this time, the mycelium is full of the...
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