Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Special culture medium for liquefaction of Pleurotus eryngii and corresponding culture method

A strain culture medium and strain culture technology are applied in mushroom cultivation, cultivation, application and other directions, which can solve the problems that liquid fermented strains cannot be applied on a large scale, increase the risk of fruiting bag pollution, and produce fruiting bag pollution accidents. , to achieve the effect of eliminating secondary pollution, reducing the amount of inoculation, and shortening the cycle.

Active Publication Date: 2017-05-31
ZHEJIANG UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Remarks: The liquid fermentation strain process theoretically has a high purity of the strain, and the bacterial contamination rate of large-scale fermentation production is between 1% and 5% (in a 3-stage fermenter), and the bacterial contamination (mainly bacterial) is often In the later stage of fermentation, it is difficult to find out by conventional on-line inspection, which leads to production accidents caused by the inoculation of liquid fermentation strains and the contamination of large quantities of mushroom bags, which is a painful lesson.
In addition, because the cultivation of liquid strains requires nutrients such as high-concentration organic nitrogen and sugar sources, these ingredients remain in the bacterial liquid and are inserted into the fruiting bag. Since bacteria are inevitably brought in during operation, the residual nutrients It becomes a breeding ground for miscellaneous bacteria, which increases the risk of contamination after inoculation of the fruiting bag
This is the main problem that liquid fermentation strains cannot be applied to the production of edible fungi on a large scale

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Special culture medium for liquefaction of Pleurotus eryngii and corresponding culture method
  • Special culture medium for liquefaction of Pleurotus eryngii and corresponding culture method
  • Special culture medium for liquefaction of Pleurotus eryngii and corresponding culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, a kind of pleurotus eryngii liquefaction special strain culture medium, it is made up of the composition of following parts by weight:

[0039]

[0040] The preparation method of above-mentioned culture medium is to carry out following steps successively:

[0041] In a stainless steel container, cornstarch, fine bran (wheat bran), and wheat cellulose are mixed uniformly under dry conditions to obtain ingredient I;

[0042] Add glucose, yeast extract, beef peptone, potassium dihydrogen phosphate, and magnesium sulfate into water, and fully stir (dissolve glucose, yeast extract, beef peptone, potassium dihydrogen phosphate, and magnesium sulfate in water) to obtain ingredient II;

[0043] After ingredients I and II are fully mixed, a culture medium specially used for the liquefaction of Pleurotus eryngii is obtained.

[0044] Remarks: The special culture medium for Pleurotus eryngii liquefaction is prepared and used now.

Embodiment 2

[0045] Embodiment 2, the Pleurotus eryngii liquefaction strain culture method that utilizes the substratum of embodiment 1 gained to carry out, the pleurotus eryngii female kind is carried out following steps successively:

[0046] 1), strain production:

[0047] 200g of the special strain culture medium for pleurotus eryngii liquefaction that has been prepared is immediately distributed in the special culture bottle of 200ml, and the microporous membrane ventilated cover is covered (selecting Shanghai Songtuo Industrial Co., Ltd. ZP14-200 ventilated culture bottle), in Carry out high temperature and high pressure sterilization at 121°C and 0.11Mpa for 90 minutes; obtain the sterilized medium;

[0048] Inject 5 grams of Pleurotus eryngii parent species (solid parent species) into the above-mentioned sterilized medium under sterile conditions, and culture in the dark at 22°C for 20 days to obtain solid strains dedicated to liquefaction; at this time, the mycelium is full of the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a special strain culture medium for pleurotus eryngii liquefaction. The special strain culture medium is prepared from the following components in parts by weight: 55-60 parts of corn starch, 1-2 parts of glucose, 5-10 parts of wheat cellulose, 20-25 parts of fine bran, 1-1. 5 parts of a yeast extract, 1-1.5 parts of beef peptone, 2-3 parts of potassium dihydrogen phosphate, 1-1.5 parts of magnesium sulfate and 100 parts of water. The invention further discloses a preparation method of the culture method, and simultaneously provides a strain culture method for carrying out pleurotus eryngii liquefaction by employing the culture medium. The culture method sequentially comprises the following steps: sterilizing a special strain culture medium for pleurotus eryngii liquefaction; inoculating the sterilized strain culture medium into a pleurotus eryngii mother strain in the sterilized culture medium under a sterile condition, and carrying out darkness culture at 21-23 DEG C for 19-21 days to obtain a special solid strain for liquefaction; and liquefying the special solid strain so as to obtain the liquefied pleurotus eryngii strain.

Description

technical field [0001] The invention relates to a special strain formula and cultivation method of Pleurotus eryngii liquefaction. Background technique [0002] Edible fungus is one of the advantageous industries with the most modern agricultural development characteristics in China. This is not only because China is the world's largest producer and consumer of edible fungi, with a wide range of edible fungus production, but more importantly, the edible fungus industry is a good carrier and prominent position in the industrialization of biotechnology and the sustainable development of agriculture. At present, my country's edible fungus industry system has many factors that are not suitable for the global market economy, the most important of which are the intensification of industrial operations, low degree of specialization, backward production factors, imperfect production technology, and lack of key technology support. The most representative one is the intensive and effi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C05G1/00A01G1/04
CPCA01G18/00C12N1/14
Inventor 陈再鸣何伯伟陈青余维良郑明海
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products