Rapid marsdenia tenacissima propagation method

A fast technology for clearance rattan, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seed reproduction rate of clearance rattan, susceptibility to cutting propagation seedlings, long growth cycle, etc., and achieves suitable scale. The effect of producing, relieving market drug pressure, and high rooting rate

Inactive Publication Date: 2015-04-29
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of low seed reproduction rate, long growth cycle, large variability, and easy susceptibility o

Method used

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  • Rapid marsdenia tenacissima propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Selection and treatment of explants

[0024] The explants are derived from the Tongguan vine plant in the wild in Mengzi County, Yunnan Province. The new shoots of the year that are free from diseases and insect pests are selected, and cut into single-bud stem segments with one bud as explants, and are initially sterilized in the following order: Rinse with running water for 2.5 hours, soak in water with a mass fraction of 5% washing powder for 5 minutes, and rinse under running water for 25 minutes; soak the customs-clearing cane section that has undergone preliminary sterilization treatment with a volume fraction of 75% ethanol solution for 1 minute, and then use mass fraction After soaking in 0.1% mercuric chloride solution for 12 minutes, wash with sterile water for 3 to 5 times, and use sterile paper to dry the surface moisture of the cane section for future use.

[0025] Because the twigs of Tongguan vine are densely covered with light yellow fluff, a lot of d...

Embodiment 2

[0039] Embodiment 2 Except that the following steps are different, the operation of other steps is the same as that of Embodiment 1, and will not be repeated here.

[0040] (1) Selection and sterilization of explants

[0041] The explants came from the wild vine plants of Mengzi County, Yunnan Province, and were transplanted to the new shoots that grew in the same year after domestication in the greenhouse of Yunnan Agricultural University. Other disinfection treatment steps are consistent with the explant treatment method in Example 1, the difference is that the disinfection time is 11 minutes with a mass fraction of 0.1% mercury chloride, and the lowest pollution rate can be achieved.

Embodiment 3

[0043] Embodiment 3 Except that the following steps are different, the operation of other steps is the same as that of Embodiment 1, and will not be repeated here.

[0044] (1) Selection and sterilization of explants

[0045] The explants are derived from the Tongguan vine plants in the wild in Maguan County, Yunnan Province. The young shoots of the year that are free from diseases and insect pests are selected and cut into single-bud stems with one bud as explants. The mass fraction is 0.1% mercuric chloride When the disinfection time was 12 minutes, the contamination rate reached the lowest. Its disinfection time is consistent with that of the explants in Example 1 from Mengzi County, Yunnan Province, which is related to the fact that they all come from the wild and have a lot of dust and germs on the plants.

[0046] See Table 2 for the test results of Embodiment 1 to Embodiment 3.

[0047] Table 2 The test effect of embodiment 1-embodiment 3

[0048]

[0049] Through...

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Abstract

The invention discloses a rapid marsdenia tenacissima propagation method and belongs to the technical field of vegetative propagation. The method comprises the following steps: selecting fresh tip of marsdenia tenacissima in the same year as an explant, washing by using running water, soaking by using washing powder water, soaking by using ethanol solution, soaking by using 0.1 mass percent of mercury bichloride solution for 11-12 minutes, and performing induction culture, enrichment culture, strong seedling culture and rooting culture. According to the method disclosed by the invention, the induction rate is over 88 percent, the marsdenia tenacissima can be propagated by about 2.5 times per month, the rooting percentage is over 95 percent, the whole propagation period is only 64-72 days, effective and rapid vegetative propagation of the marsdenia tenacissima is realized, and the method is suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and in particular relates to a method for rapid propagation of Chinese herbal medicine Tengguan vine. Background technique [0002] Tongguan vine (Marsdenia tenocissima (Roxb) Wight et Arm) is a vine of the genus Milkweed in the Ascaridaceae family, also known as black bone vine. It was first recorded in "Southern Yunnan Materia Medica", and is widely distributed in tropical and subtropical regions of Asia. The whole plant has the effects of clearing away heat and detoxification, reducing inflammation and relieving pain, relieving cough and relieving asthma. Wuguxiaocarcin contained in Tongguanteng (C 21 - Steroidal glycosides and total alkaloids) have a strong destructive effect on the microtubule arrangement, the root of cancer cell division, and can block the mitosis of cancer cells, thereby effectively cutting off the proliferation and metastasis of cancer cells, so tha...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韩俊杨生超颜秋燕候建凯龙光强黄鹤李连文王自林沈雪梅杨丕琼
Owner YUNNAN AGRICULTURAL UNIVERSITY
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