Peak shape delaying forward suppressor and high-performance liquid chromatographic analysis device and method using the same

Abstract

The invention discloses a peak shape delaying forward suppressor for a high-performance liquid chromatographic analysis device and method. The peak shape delaying forward suppressor comprises a cutting sleeve and a suppressor core arranged in the interior of the cutting sleeve, the suppressor core is of a hollow structure and the interior of the suppressor core is provided with cavities, the end portions of the interior of the suppressor core are provided with screen plates, and the screen plates are communicated with the cavities. The invention further discloses the high-performance liquid chromatographic analysis device and method which use the peak shape delaying forward suppressor, and when sample solvents with samples flow through the cavities, the sample solvents can be diluted by the mobile phase. The peak shape delaying forward suppressor and the high-performance liquid chromatographic analysis device and method using the peak shape delaying forward suppressor has the advantages that the peak shape delaying forward phenomenon caused by the mismatching of the sample solvents and the mobile phase can be effectively suppressed, the interferences brought by the sample solvents are solved, the peak shape is improved, the column efficiency is increased, the separation degree is improved, the interferences brought by the sample solvents when the analysis, the preparation and the method development are carried out are greatly reduced, and man power and material resources are significantly saved.

Description

technical field [0001] The invention relates to the technical field of chromatographic analysis, in particular to high-performance liquid chromatography analysis and preparation, and in particular to a peak-forward suppressor, a high-performance liquid chromatography analysis device and a method using the same. Background technique [0002] High performance liquid chromatography is currently the most important means and method in the process of production supervision and quality control in biological, pharmaceutical, medical, food, chemical and other industries. The world includes biopharmaceuticals, medical diagnosis, food safety and chemical quality inspection and other fields. More than 80% of the tens of thousands of compounds in the country are analyzed by high performance liquid chromatography, which is widely involved in various fields of national economy and people's livelihood. With the development of the times and the improvement of people's living standards, peopl...

AI Technical Summary

Problems solved by technology

In the process of developing the method, many samples have poor solubility in the mobile phase. However, due to some objective conditions, it is difficult to dissolve the sample with the mobile phase. In many cases, it is necessary to use a solvent different from the mobile phase to dissolve the sample. This causes the phenomenon that the peak shape is often prone to prolongation during liquid chromatography analysis and preparation, which seriously affects the accuracy of peak area integration, resulting in a sharp decline in column efficiency and a significant reduction in resolution when liquid chromatography is used for analysis and preparation. The separation efficiency becomes worse, the content calculation is very inaccurate, and it will directly affect the separation purity in the preparation liquid phase

[0009] Such a significant impact makes some methods have to be changed, and the changing process will increase the cost of the process and pre-treatment, and some even have to give up all the previous work and start from the beginning to explore the method, wasting a lot of manpower and material resources resource

[0010] Many of the current high-performance liquid chromatography methods in the national standard and pharmacopoeia are troubled by the large difference between the composition of the "sample solvent" and the mobile phase, which makes many analysts who need to use these methods to test their products greatly confused. Headache, because the method is authoritative, it cannot be easily changed, and the "sample solvent" in these methods does not match the mobile phase, so people have to constantly try new columns without changing the original method, hoping to have a new column. This magic column will solve their problem, but it will not be easy, and even if the problem is finally solved, this process will consume a lot of time, effort, expensive solvents, etc. Columns, resulting in a huge waste of resources

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