Specific primer and identification method for microsatellite markers of sea horses in coastal waters of China

Abstract

The invention discloses a specific primer and an identification method for microsatellite markers of sea horses in coastal waters of China. The identification method comprises the following steps: firstly developing a plurality of sea horse microsatellite markers (SSR); then extracting a sea horse sample DNA; then carrying out PCR amplification on the sea horse microsatellite markers and positive control (a COI gene); carrying out agarose gel electrophoresis on a PCR product; and identifying according to strip type of a plurality of product strips. The identification method disclosed by the invention is not limited by morphological characteristics of the sea horse sample. Regardless of size, freshness or death, deformation and incompleteness of the sample, species identification of sea horses in coastal waters of China can be accomplished by a small amount of DNA, so that the identification method is strong in practicability. The identification method is clear in result, intuitive and clear, strong in repeatability and high in reliability, can be used for detecting a plurality of sea horses or types of sea horses at the same time, and is simple and convenient and efficient and convenient to operate, thereby providing a powerful tool for researches on biological resource survey, germplasm protection, ecoethology and the like on the sea horses in coastal waters of China.

Description

Technical field: [0001] The invention belongs to the field of investigation of rare fishery resources, and in particular relates to a specific primer and an identification method for the microsatellite marker of the Chinese offshore hippocampus. Background technique: [0002] Hippocampus, belonging to Syngthidae (Syngthidae) and Hippocampus (Hippocampus), is a type of bony fish. It is a precious marine Chinese medicinal material. It is known as the "Southern Ginseng" in my country. It has the effects of pain, calming and soothing, but also has high ornamental value and the function of collecting decorations. The international trade volume of seahorses is also increasing year by year. In 2009, my country imported 40 million dried seahorses, accounting for 2 / 3 of the international seahorse market, and paid more than 500 million US dollars in foreign exchange. In the past few decades, due to long-term overfishing, environmental pollution, climate change and other factors, my country...

AI Technical Summary

Problems solved by technology

The traditional method is based on hippocampal morphological data. However, this method is time-consuming and laborious. What's worse, when the hippocampus is small or may be damaged during the collection, processing, identification and storage of hippocampal specimens, it cannot Morphologically accurate identification

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