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Fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll

A technology for lowering cholesterol and bifidobacteria, applied in the field of microorganisms

Active Publication Date: 2015-04-22
奈安制药(中国)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no applications involving cholesterol-lowering, aerobic-tolerant bifidobacteria

Method used

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  • Fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll
  • Fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll
  • Fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Example 1: Bifidobacterium animalis BZ11 was screened and screened from the intestinal solubles of small fragrant pigs, a characteristic resource in Guizhou Province:

[0050] (1) Collection of samples:

[0051] Samples were taken from several parts of the piglets less than 2 months old in the ecological garden of Guiyang City, Guizhou Province, including the small intestine, large intestine and fresh piglet feces. The fresh piglet samples were placed in the place with sterilized forceps Put them in a sterile sealed bag, put them into self-refrigerated phases with ice packs after numbering, and bring them back to the laboratory for processing within 1 hour;

[0052] (2) Isolation and purification of bifidobacteria

[0053] Put 10g of fresh sample into 90mL of sterilized distilled water (containing glass beads), shake it manually and perform gradient dilution. In the ultra-clean workbench, get its sample dilution 0.1mL and join in the MRS (MUP-MRS) plate culture medium...

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Abstract

Provided is fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll. The fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll is Bifidobacterium animalis subsp.Lactis, the pure culture of the fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll is preserved at the China General Micro-biological Culture Collection Center (CGMCC), China Microbial Culture Preservation Management Committee, the address is Institute of Microbiology, Institution No.3 China Academy of Science, Western Beichen Road No.1 in Chaoyang District of Beijing in China, the postal code is 100101, the preservation number is CGMCC NO.10224, and the Bifidobacterium animalis subsp.Lactis BZll is called for short. The Bifidobacterium animalis subsp.Lactis BZll is high in cholesterol-assimilating capacity, acid resistance and cholate resistance, good in oxygen resisting performance, has probiotic property and can serve as probiotics to be added into a fermentative milk product to produce a functional food, bifidobacterium culture resources are enriched, and development of bifidobacterium health-care products is facilitated.

Description

technical field [0001] The invention relates to microorganisms, in particular to cholesterol-lowering and oxygen-resistant bifidobacteria. Background technique [0002] Cholesterol is widely present in animal tissue cells and is one of the important components of cells. It plays an important physiological role in the human body. It is transformed into steroid hormones in the body and is a precursor for the synthesis of vitamin D and bile acids. There are two main sources of cholesterol in the body, one is food, and the other is endogenous synthesis. Due to unreasonable dietary habits, some people's blood cholesterol levels exceed normal indicators; and excessive cholesterol levels in the body can cause cardiovascular and cerebrovascular diseases such as atherosclerosis and coronary heart disease, which seriously threaten human health. According to reports, the number of human deaths caused by cardiovascular and cerebrovascular diseases in the world accounts for 29% of the t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61P3/06C12R1/01
CPCC12N1/205C12R2001/01
Inventor 何腊平张玲
Owner 奈安制药(中国)有限公司
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