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Lactobacillus plantarum with function of removing patulin

A technology of Lactobacillus plantarum and patulin, applied in the field of microorganisms, can solve the problems of low efficiency, seldom application, and limited removal of patulin, and achieve good growth, wide application prospects, and good tolerance to bile salts

Active Publication Date: 2015-04-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the efficiency of removing patulin by microorganisms is generally not high, and it is limited to removing patulin in aqueous solution or culture medium, and is rarely used in food and feed

Method used

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  • Lactobacillus plantarum with function of removing patulin
  • Lactobacillus plantarum with function of removing patulin
  • Lactobacillus plantarum with function of removing patulin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The drawing of the growth curve of embodiment 1 Lactobacillus plantarum CGMCC No.9511

[0029] Take the Lactobacillus plantarum CGMCC No.9511 stored at -20°C, and streak it on the MRS plate. After culturing at 37°C for 48 hours, pick lactic acid bacteria cells from a well-growing single colony of Lactobacillus plantarum and inoculate them into MRS liquid medium for shaking culture for 24 hours at a culture temperature of 37°C and a shaker speed of 150 rpm . Plantarum CGMCC No.9511 culture fluid inoculated into MRS liquid culture medium by 2% inoculum amount after this activation is then at a temperature of 37 ° C, and the rotating speed is 24 hours in a shaking table of 150 r / min, and every 2 Take 5mL of culture solution in 1 hour and use a UV spectrophotometer to measure the OD value at 600nm and carry out plate counting, draw the growth curve of Lactobacillus plantarum CGMCC No.9511 according to the results, the results are as follows figure 1 shown, from figure 1 ...

Embodiment 2

[0030] Example 2 The preparation method of the Lactobacillus plantarum CGMCC No.9511 biological agent that clears patulin

[0031] Inoculate Lactobacillus plantarum CGMCC No.9511 in the MRS medium with 2% inoculum amount, culture it in a shaker with a temperature of 30-37°C and a shaker speed of 150r / min-200r / min for 18-24h, then pass through 5000r / min, centrifuged for 10 min to obtain Lactobacillus plantarum cells, washed the centrifuged cells with 0.85% saline three times, and then centrifuged to obtain Lactobacillus plantarum CGMCC No.9511 biological preparation. Or resuspend the bacteria with a protective agent to reach a concentration of 10 10 CFU / mL, the protective agent is 100g / L skimmed milk powder, 100g / L trehalose and 150g / L sucrose, then, let the suspension be pre-incubated for 60min at a temperature of 37°C, and then pre-incubated at -20°C. Freeze for 2-4 hours, and finally freeze-dry to obtain the biological preparation of Lactobacillus plantarum CGMCC No.9511 b...

Embodiment 3

[0032] The effect of embodiment 3 patulin on the growth of Lactobacillus plantarum CGMCC No.9511

[0033] 1. Preparation of patulin standard stock solution

[0034] The patulin is a standard product of patulin, purchased from Beijing Taileqi Technology Co., Ltd., and prepared into a 100mg / L mother solution during use. The preparation method is as follows: Accurately weigh 5 mg of patulin standard substance, dissolve it in 50 mL of ethyl acetate, mix well and dissolve, filter and sterilize with a 0.22 μm microporous membrane, and store at -20°C.

[0035] 2. Preparation of MRS culture medium containing different concentrations of patulin

[0036]According to the needs, accurately measure a certain volume of patulin standard stock solution with a concentration of 100mg / L, dry it with nitrogen, dissolve it in the MRS culture medium, and set the volume to 100mL. For example, when preparing a patulin culture solution with a concentration of 1000 μg / L, first accurately measure 1 mL...

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Abstract

The invention discloses lactobacillus plantarum with a function of removing patulin and belongs to the technical field of microorganisms. The lactobacillus plantarum disclosed by the invention has acid resistance and can be used for removing patulin from an aqueous solution. When 1*10<10>CFU / mL of lactobacillus plantarum is cultured together with 1000mu g / L patulin for 4 hours, the lactobacillus plantarum can remove more than 80% of the patulin, and when 1*10<10>CFU / mL of lactobacillus plantarum is cultured together with 1000mug / L patulin for 8 hours, the lactobacillus plantarum can remove more than 90% of the patulin. In addition, the lactobacillus plantarum has the functions of significantly reducing the patulin in apple juice and moldy apple pomace and alleviating the influence of the patulin on intestinal microflora of mice. The lactobacillus plantarum is used for removing patulin in foods or feeds and has very broad application prospect.

Description

technical field [0001] The invention relates to a plant lactobacillus capable of removing patulin and belongs to the technical field of microorganisms. Background technique [0002] Patulin, also known as patulin, is a toxic fungal metabolite produced by the metabolism of molds such as Penicillium, Aspergillus, and silkworm. It has extensive and strong toxic effects on humans and animals. Acute toxicity manifests as convulsions, convulsions, subcutaneous tissue edema, pulmonary and intestinal congestion, enteritis, anuria, and death; chronic toxicity can lead to gastric ulcers, immunosuppression, teratogenicity, and potential carcinogenicity. Patulin is soluble in water, ethanol, acetone, ethyl acetate and chloroform, slightly soluble in ether and benzene, insoluble in petroleum ether, and chemically stable under acidic conditions. [0003] Patulin is a worldwide pollutant. The contamination of patulin in food and feed production is quite common. It is widely found in prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A23L1/015A23K1/14A23K3/02C12R1/25C02F101/34A23L5/20
CPCC02F3/34C02F2101/34C12N1/205C12R2001/25
Inventor 陈卫田丰伟龚雪翟齐啸王刚赵建新张灏
Owner JIANGNAN UNIV
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