Method for treating waste water containing aniline blue with cultivation material after harvesting Pleurotus eryngii
A cultivation material treatment, aniline blue technology, applied in the directions of water pollutants, chemical instruments and methods, textile industry wastewater treatment, etc., can solve the problems that cultivation waste cannot be recycled and high treatment costs, and achieve waste reuse and purification. The effect of environment and good development prospects
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Embodiment 1
[0012] Example 1: Study the effect of different metal ions on enzyme activity.
[0013] Pleurotus eryngii cultivated material was provided by the Laboratory of Microbiology Engineering, College of Life Sciences, Fujian Agriculture and Forestry University. The results of measuring enzyme activity of cultivated material after harvest of Pleurotus eryngii showed that Pleurotus eryngii secreted laccase, manganese peroxidase, cellulase and hemicellulase, but not lignin peroxidase.
[0014] When Mn 2+ When the concentration is 2.5 mmol / L, laccase is activated; Ag + When the concentration is 5 mmol / L, both laccase and manganese peroxidase are inhibited; Fe 2+ At a concentration of 2.5 mmol / L, it inhibits laccase and promotes the secretion of manganese peroxidase, Ag + , Fe 2+ It has a strong inhibitory effect on laccase. Add 2.5 mmol / L Mn respectively 2+ , 5 mmol / L Ag + , 2.5 mmol / L Fe 2+ After the decolorization of aniline blue, the result showed that adding 2.5 mmol / L Mn 2+ The syste...
Embodiment 2
[0015] Example 2: Optimization of decolorization conditions for aniline blue in cultivation material of Pleurotus eryngii after harvest
[0016] After the harvested Pleurotus eryngii cultivated material is pulverized with a pulverizer and mixed, 6 g are respectively weighed and put into a 100 mL reaction system, shaken in a constant temperature shaker, and regularly sampled to verify the temperature, pH, speed, and dye Concentration, mediator molecule, Mn 2+ The effect of concentration on the decolorization of aniline blue. Observe the decolorization to measure its absorbance and calculate the decolorization rate.
[0017] The results are as follows:
[0018] 1. Temperature: the result when the reaction system is decolorized for 210 minutes. When the temperature is from 10-30℃, the decolorization rate of aniline blue will increase with the increase of temperature. When the temperature is higher than 30℃, the decolorization rate will decrease with the increase of temperature. If the ...
Embodiment 3
[0027] Example 3: Treatment of printing and dyeing wastewater and toxicological experiment with cultivation material after harvest of Pleurotus eryngii
[0028] The UV scanning spectra before and after dye degradation showed that the characteristic absorption peak of aniline blue at 585 nm disappeared, indicating that the cultivated material after harvest of Pleurotus eryngii can degrade aniline blue. Under the best decolorization conditions, the removal rate of aniline blue COD was 53.6%. The results of degradation of industrial printing and dyeing wastewater by cultivated material after harvest of Pleurotus eryngii showed that the amount of cultivated material added was positively correlated with the COD removal rate of printing and dyeing wastewater. At 30℃, 400 μmol / L ABTS, 5 mmol / L Mn 2+ , Under the condition of 110 rpm, the COD removal rate reached 86.1%. The results of toxicological experiments showed that the germination rates of rice seeds in solution culture before and...
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