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Ganoderma tsugae strain cultivation method

A technology of pine-fir ganoderma and its cultivation method, which is applied in the field of cultivation of pine-fir ganoderma strains, can solve the problems of decreased drug properties, low yield of pure cotton seed hulls, unstable safety of ganoderma, etc., and achieves increased content of active ingredients, good economical Effects of benefit and social benefit, good economic benefit and social benefit

Inactive Publication Date: 2015-04-08
ZHISHENG TIANJIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of pure cotton seed husks is low and the medicinal properties are reduced, while the production of Ganoderma lucidum with weeds and traditional Chinese medicine residues as raw materials is unstable.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A kind of cultivation method of pine fir ganoderma strain, comprises the following steps:

[0015] (1) Production of parent species: Collect the strains of Pinus fir ganoderma lucidum isolated from tissues, connect the strains into the sterilized short basswood cavity, and place them in a 30°C incubator for constant temperature and dark cultivation until the mycelium is covered with short basswood Cavity can produce female species, wherein the formula of medium on the short basswood cavity is: broken wheat 20kg, wheat bran 5kg, agar 2kg, calcium carbonate 0.3kg, calcium lactate 0.4kg, magnesium sulfate 0.15 kg, vitamins 0.01kg, humic acid 0.10kg, the rest is water, water content 55-60%;

[0016] (2) Making the original seed: Aseptically insert the production mother seed into the original seed bag, place it in a 25°C incubator for constant temperature and dark cultivation, and obtain the original seed after the mycelium is full of materials, and the culture medium of the...

Embodiment 2

[0020] A kind of cultivation method of pine fir ganoderma strain, comprises the following steps:

[0021] (1) Production of parent species: Collect the strains of Pinus fir ganoderma lucidum isolated from tissues, connect the strains into the sterilized short basswood cavity, and place them in a 30°C incubator for constant temperature and dark cultivation until the mycelium is covered with short basswood Cavities promptly obtain the production female species, wherein the formula of the culture medium on the short basswood cavities is calculated as broken wheat 25kg, wheat bran 3kg, agar 4kg, calcium carbonate 0.5kg, calcium lactate 0.5kg, Magnesium sulfate 0.20kg, vitamin 0.02kg, humic acid 0.13kg, water content 55-60%;

[0022] (2) Making the original seed: Aseptically insert the production mother seed into the original seed bag, place it in a 25°C incubator for constant temperature and dark cultivation, and obtain the original seed after the mycelium is full of materials, an...

Embodiment 3

[0026] A kind of cultivation method of pine fir ganoderma strain, comprises the following steps:

[0027] (1) Production of parent species: Collect the strains of Pinus fir ganoderma lucidum isolated from tissues, connect the strains into the sterilized short basswood cavity, and place them in a 30°C incubator for constant temperature and dark cultivation until the mycelium is covered with short basswood Cavities promptly obtain the production female species, wherein the formula of the culture medium on the short basswood cavities is calculated as broken wheat 30kg, wheat bran 5kg, agar 5kg, calcium carbonate 0.7kg, calcium lactate 0.6kg, Magnesium sulfate 0.25kg, vitamin 0.03kg, humic acid 0.15kg, water content 55-60%;

[0028] (2) Making the original seed: Aseptically insert the production mother seed into the original seed bag, place it in a 25°C incubator for constant temperature and dark cultivation, and obtain the original seed after the mycelium is full of materials, an...

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Abstract

The invention provides a ganoderma tsugae strain cultivation method and particularly relates to the technical field of cultivation and processing of fungi. The method includes the four steps of mother strain cultivation, stock culture production, inoculation cultivation and fruiting management. The cultivated ganoderma tsugae strain is high in content of active ingredient and good in anti-tumor function and has good economic and social benefits, and the cultivation method is economical, practical, low in cost and good in effect; due to substrates mainly composed of grain materials and addition of humic acid, poison to the strain due to heavy metal in soil is reduced; the technique of simulating the growth environment of the wild ganoderma tsugae is adopted, so that yield of the ganoderma tsugae is increased while quality of the same is improved, and safety of the ganoderma tsugae products is guaranteed. The method is beneficial to standardization, industrialization and internationalization of the traditional Chinese medicine ganoderma tsugae products; quality of the ganoderma tsugae is improved highly, the active ingredient content is increased significantly, and good economic and social benefits are gained.

Description

technical field [0001] The invention belongs to the technical field of fungi cultivation and processing, and in particular relates to a method for cultivating pine fir ganoderma strains. Background technique [0002] Pine and fir Ganoderma lucidum is included in the Pharmacopoeia of Traditional Chinese Medicine in my country, and it is widely used in anti-tumor drugs and health food together with Chizhi and Zizhi. After years of fluctuating development, Songshan Ganoderma lucidum has developed from one-way export to domestic and foreign sales of various processed products. The wild pine and fir ganoderma is sought after by people because of its unique growth environment and the traditional concept of green safety, and the price has always remained high. At present, the patent applications on Ganoderma lucidum are mainly related to the extraction of Ganoderma lucidum, preparation of polysaccharides, molecular markers of fruiting bodies or cultivation methods of existing stra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00C05D3/02C05G3/00
Inventor 季延滨李涛
Owner ZHISHENG TIANJIN BIOTECH
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