Production method of ICSI operation vessel

Abstract

The invention discloses a production method of ICSI operation vessel. The production method comprises the following steps under the following conditions: sucking PVP solution by a suction tube, forming two 10 microliter circular micro-droplets above the ICSI vessel and forming a strip-shaped PVP micro-droplet with a length of 2.5cm and a width of 0.5cm under the ICSI vessel; sucking about 0.1ml of a culture medium containing sperms, and forming a strip-shaped micro-droplet with a length of about 2.0cm and a width of about 0.5cm at one side of the strip-shaped PVP micro-droplet; sucking a buffer culture medium, and forming three to five 10 microliter circular micro-droplets containing an ovum at the other side of the strip-shaped PVP micro-droplet; sucking the buffer culture medium, and forming one 10 microliter circular micro-droplet under the strip-shaped PVP micro-droplet; sucking one sperm from the strip-shaped micro-droplet of the culture medium containing sperms by a microscopic puncture needle, placing the sperm in the strip-shaped PVP micro-droplet, and immobilizing the sperm; fixing the ovum by a microscopic fixation needle so that the polar bodies of the ovum can be located at a six-o'clock position or a twelve-o'clock position; injecting the sperm in the ovum by the microscopic puncture needle with the sperm from a three-o'clock position.

Description

technical field [0001] The invention relates to a method capable of finding and absorbing sperm in a short time, in particular to a method for making an ICSI operating dish. Background technique [0002] Intracytoplasmic sperm injection (ICSI) has enabled countless patients with severe oligospermia and obstructive azoospermia to have their own offspring. The ICSI operation process must be completed on the ICSI operation dish. The traditional ICSI operation dish consists of ICSI dish, PVP It is composed of circular micro-droplets, PVP strip-shaped micro-droplets and buffer medium micro-droplets. The process is to absorb about 1.0 μl of the medium containing sperm and add it to the PVP strip-shaped micro-droplets. After braking, the ICSI operation is completed, but some In the case of extremely low sperm density after washing, it is difficult to find sperm in a short time using this method, which greatly prolongs the operation time of ICSI. The eggs are exposed to the outside ...

AI Technical Summary

Problems solved by technology

[0002] Intracytoplasmic sperm injection (ICSI) has enabled countless patients with severe oligospermia and obstructive azoospermia to have their own offspring. The ICSI operation process must be completed on the ICSI operation dish. The traditional ICSI operation dish consists of ICSI dish, PVP It is composed of circular micro-droplets, PVP strip-shaped micro-droplets and buffer medium micro-droplets. The process is to absorb about 1.0 μl of the medium containing sperm and add it to the PVP strip-shaped micro-droplets. After braking, the ICSI operation is completed, but some In the case of extremely low sperm density after washing, it is difficult to find sperm in a short time using this method, which greatly prolongs the operation time of ICSI. The eggs are exposed to the outside of the incubator for a long time, and the constant temperature, humidity and CO2 concentration cannot be maintained. Damage the ultrastructure of the egg, affecting its fertilization and developmental potential. If the amount of medium added is too large, the total number of sperm will increase, and it is relatively easy to find sperm, but it will cause the sperm to float in the PVP solution, and the puncture needle is difficult to grasp Taking and immobilizing sperm will also prolong the ICSI operation time, and some sperm impurities in the medium will contaminate the PVP solution

[0003] Some IVF laboratories recommend that patients with severe oligozoospermia and obstructive azoospermia receive multiple sperm collections and then freeze them to increase the total amount of sperm. The disadvantages of this method are: multiple times of freezing sperm will increase the cost of treatment. The recovery effect after freezing is not ideal, and whether the freezing liquid will damage the sperm, whether liquid nitrogen will contaminate the sperm during the freezing process, and the safety of using the recovered sperm are still inconclusive

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