Wall-breaking method for haematococcus pluvialis cells

Abstract

The invention belongs to the technical field of microalgae microorganisms and relates to a wall-breaking method for haematococcus pluvialis cells which are rich in a biological active substance: astaxanthin. The wall-breaking method for the haematococcus pluvialis cells provided by the invention comprises the following three steps: 1, freezing and ice-crystallizing the haematococcus pluvialis cells and dispersing the cells in a flowing liquid below 0 DEG C; 2, adding a bioactive component which protects the haematococcus pluvialis into the liquid before the ice-crystallized algae cells are broken so as to reduce a protectant which is oxidized and decomposed after wall breaking; and 3, after uniformly grinding by a colloid mill, carrying out ultra-high-pressure wall-breaking by using an ultra-high-pressure homogeneous nanometer machine. The wall-breaking process provided by the invention is carried out under a condition of low-temperature, cold chain and close away from light and breaks walls by means of the characteristic of nanometer crushing by using the ultra-high-pressure homogeneous nanometer machine and has the advantages of rapidness, efficiency and no addition of foreign matters and can be used for effectively protecting the bioactive component.

Description

Technical field [0001] The present invention is a microalgae biotechnology field, which involves a wall breaking technology that is rich in rain -raining red gluten algae cells rich in shrimp greenin biological activated substances. Background technique [0002] Rain -ray red ball algae is a single -cell green algae living in freshwater. Under certain conditions, it can accumulate a large amount of carotene, more than 80%of which are shrimp and ester.Shrimp greenin has important physiological and biological functions such as antioxidant, anti -tumor, and increasing immunity. [0003] Rhizon red ball algae cells have cellulose cell walls with cell wall thickness, generally 3-5 microns, and the texture is tough.With the advancement of cultivation technology, the content of shrimp greenin has increased significantly, which has exceeded 5%, and can even reach more than 6%.In order to better protect themselves, the more cellulose in its cells, the thicker the cell wall, the more tough...

AI Technical Summary

Problems solved by technology

With the advancement of culture technology, the content of astaxanthin has increased significantly, breaking through 5%, and even reaching more than 6%. The theoretical output of astaxanthin has doubled, but the higher the content of astaxanthin, the more the In order to better protect themselves, the cell cellulose increases, the thicker and tougher the cell wall has reached 6-8 microns, and it is more difficult to break. The rate of cell breakage directly affects the extraction rate of astaxanthin, directly affecting the actual output. If the general method of breaking the wall is used, such as freeze-thaw method, ultrasonic method, biological enzymatic hydrolysis method, high-pressure homogenizer, colloid mill, etc., one or more confluences are used to break the wall, and the breaking rate can only reach 80-85%. , so the wall-breaking technology has seriously restricted the large-scale development of Haematococcus pluvialis cultivation and the expansion of the subsequent astaxanthin industry chain. Now it has become the most breakthrough core issue of Haematococcus pluvialis development technology

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