Effective sexual hybridization technology of abelmoschus esculentus
A kind of hybridization technology, a sexual technology, applied in the field of efficient sexual hybridization technology of okra, can solve the problems of time-consuming and laborious, and achieve the effect of improving efficiency, simple method, and reducing the difficulty of pollination
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Embodiment 1
[0022] At 7 o'clock, collect flower buds from the male parent flower of Qingfu okra variety, remove the corolla, spread it flat on sulfuric acid paper, place it in a cool, dry and ventilated place, collect it after the pollen is scattered, and remove impurities with a 75 micron sieve. The pure pollen is obtained, and the pure pollen is placed in a light-proof bottle and sealed, and stored at a low temperature and protected from light until use. Put pollen, indole butyric acid, white granulated sugar, boric acid and gum arabic into water respectively, ultrasonicate for 25 minutes, and stir evenly to obtain a pollen suspension for use. The concentration of pollen in the pollen suspension is 3g / L, and indole butyric acid 2g / L for white sugar, 2g / L for boric acid, and 1g / L for gum arabic.
[0023] 2 days before the okra female parent flower blooms, the stigma part is isolated by bagging, and at 8 o'clock on the first day of flowering, the isolate is removed, and the pollen suspens...
Embodiment 2
[0025] At 10 o'clock, collect flower buds from the male parent flower of Wufu okra variety, remove the corolla, spread it flat on sulfuric acid paper, place it in a cool, dry and ventilated place, collect it after the pollen is scattered, and use a 100-micron sieve to remove impurities. The pure pollen is obtained, and the pure pollen is placed in a light-proof bottle and sealed, and stored at a low temperature and protected from light until use. Put the pollen, indole butyric acid, white granulated sugar, boric acid and gum arabic into water respectively, ultrasonicate for 30 minutes, and stir evenly to obtain a pollen suspension for use. The concentration of pollen in the pollen suspension is 3g / L, and indole butyric acid 2g / L for white sugar, 2g / L for boric acid, and 1g / L for gum arabic.
[0026] The stigma part is isolated by bagging 3 days before the flowering of the okra female parent flower, and at 15 o'clock on the first day of flowering, the isolater is removed, and t...
Embodiment 3
[0028] At 8 o'clock, collect flower buds from the male parent flower of the Nanyang okra variety, remove the corolla, spread it flat on sulfuric acid paper, place it in a cool, dry and ventilated place, collect it after the pollen is scattered, and remove impurities with an 80-micron sieve. The pure pollen is obtained, and the pure pollen is placed in a light-proof bottle and sealed, and stored at a low temperature and protected from light until use. Put the pollen, indole butyric acid, white granulated sugar, boric acid and gum arabic into water respectively, ultrasonicate for 30 minutes, and stir evenly to obtain a pollen suspension for use. The concentration of pollen in the pollen suspension is 3g / L, and indole butyric acid 2g / L for white sugar, 2g / L for boric acid, and 1g / L for gum arabic.
[0029] The stigma part is isolated by bagging 2 days before the flowering of the female parent flower of okra, and at 9 o'clock on the first day of flowering, the isolater is removed,...
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