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Construction method and standard fingerprint of Lycium barbarum polysaccharide multivariate fingerprint

A technology of standard fingerprints and Lycium barbarum polysaccharides, applied in the field of standard fingerprints of Lycium barbarum polysaccharides, can solve problems such as difficult control and identification, imperfect detection methods and quality standards, and uneven product quality

Active Publication Date: 2016-04-06
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Lycium barbarum polysaccharide is a natural biopolymer with a complex structure, and its scientific and comprehensive detection methods and quality standards are not yet perfect, so that the quality of Lycium barbarum, Lycium barbarum polysaccharide and related products is uneven, difficult to control and identify

Method used

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  • Construction method and standard fingerprint of Lycium barbarum polysaccharide multivariate fingerprint
  • Construction method and standard fingerprint of Lycium barbarum polysaccharide multivariate fingerprint
  • Construction method and standard fingerprint of Lycium barbarum polysaccharide multivariate fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment 1, the establishment method of Lycium barbarum polysaccharide multiple fingerprint

[0095] 1. Instruments, reagents and samples

[0096] 1.1 Instrument

[0097] Shimadzu UV-2401PC UV Vis Spectrophotometer

[0098] BRUKER-MPA Infrared Spectrometer

[0099] Shimadzu LC-20AT high performance liquid chromatograph equipped with diode array detector (DAD) and Shimadzu chromatography workstation.

[0100] 1.2 Reagents:

[0101]

[0102] 1.3 Lycium barbarum raw materials:

[0103] 16 batches of wolfberry raw materials come from different producing areas such as Ningxia, Xinjiang, Qinghai, Henan, Hebei, Gansu, and Shaanxi.

[0104] 2. Pretreatment of wolfberry

[0105] Accurately weigh 120g of wolfberry, spread it evenly on a porcelain plate, bake at 60°C for 6 hours to make it completely dry, crush the dried wolfberry with a grinder, and weigh M 1 g. Will M 1 Put g wolfberry powder in a 1000mL flask, add 3 times the volume of 80% ethanol, reflux at 85°C ...

Embodiment 2

[0141] Example 2, Multivariate fingerprint analysis of Ningqi No. 7 Lycium barbarum polysaccharide sample from a certain origin in Ningxia

[0142] 1. Pretreatment of wolfberry

[0143] Accurately weigh 120 g of wolfberry fruit, spread it evenly on a porcelain plate, bake at 60°C for 6 hours to make it completely dry, and crush the dried wolfberry fruit with a grinder, weighing 117g. Put 117g wolfberry powder in a 1000mL flask, add 351mL of 80% ethanol, reflux at 85°C for 2h, cool, drain and discard the solvent, place in a 1000mL flask, add 263mL of 80% ethanol, reflux at 85°C for 2h, cool, drain and discard Solvent, drying, weighing 53g. Put 53 g of the sample in a Soxhlet extractor, add 318 mL of chloroform-methanol (chloroform:methanol=2:1) ​​mixed solution, reflux at 70°C for 4 hours, drain to remove the solvent, and dry to obtain 51 g of defatted Lycium barbarum powder.

[0144] 2. Extraction of Lycium barbarum polysaccharide

[0145] Put 51g of wolfberry coarse powder...

Embodiment 3

[0166] Example 3, Multivariate fingerprint analysis of Lycium barbarum polysaccharide samples from a certain producing area in Shanxi

[0167] 1. Pretreatment of wolfberry

[0168] Accurately weigh 120g of wolfberry fruit, spread it evenly on a porcelain plate, bake at 60°C for 6 hours to make it completely dry, and crush the dried wolfberry fruit with a grinder, weighing 116.5g. Put 116.5g wolfberry powder in a 1000mL flask, add 350mL of 80% ethanol, reflux at 85°C for 2h, cool, drain and discard the solvent, put it in a 1000mL flask, add 262mL of 80% ethanol, reflux at 85°C for 2h, cool, pump The solvent was discarded, dried, and the weight was 48g. Put 48g of the sample in a Soxhlet extractor, add 288mL of chloroform methanol (chloroform:methanol=2:1) ​​mixed solution, reflux at 70°C for 4h, drain the solvent, and dry to obtain 47g of defatted Lycium barbarum powder.

[0169] 2. Extraction of Lycium barbarum polysaccharide

[0170] Put 47g of wolfberry coarse powder into...

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Abstract

The invention provides a construction method of a wolfberry fruit polysaccharide multi-element fingerprint spectrum and a wolfberry fruit polysaccharide standard fingerprint spectrum, belonging to the technical field of traditional Chinese medicine and functional food raw materials and a fingerprint spectrum of the product. The construction method comprises the following steps: pretreating wolfberry fruits, extracting wolfberry fruit polysaccharide, determining a UV standard fingerprint spectrum, determining an HPSEC standard fingerprint spectrum, analyzing an IR light spectrum fingerprint and determining the standard fingerprint spectrum, completely hydrolyzing, deriving a hydrolyzed product, and analyzing a PCD-HPLC fingerprint and determining the standard fingerprint spectrum. The pretreatment, extraction of the wolfberry fruit polysaccharide and construction method of the multi-element fingerprint spectrum of 16 types of wolfberry fruits in different production areas are the same as above and can be operated simultaneously and respectively. The construction method of the wolfberry fruit polysaccharide multi-element fingerprint spectrum has the advantages of simplicity in operation, stability, flexibility, high precision, good repeatability and the like; and the quality condition and production area resources of the wolfberry fruit polysaccharides can be grasped from a whole characteristic appearance of a chromatogram and a novel scientific method is provided for wolfberry fruit quality control and true and false identification.

Description

technical field [0001] The invention relates to a method for establishing four kinds of fingerprints of Lycium barbarum polysaccharide, which are UV, HPSEC, IR and PCD-HPLC fingerprints of Lycium barbarum polysaccharide, and also relates to a standard fingerprint of Lycium barbarum polysaccharide obtained by the method. The invention belongs to the technical field of fingerprints of traditional Chinese medicine and its products, functional food raw materials and its products. Background technique [0002] Lycium barbarum is the mature fruit of Lycium barbarum, a plant of the family Solanaceae. It is also known as Western wolfberry, Nitraria, Mountain wolfberry, and Baipizhen. Thirst, nourishes the lungs and relieves coughs. It is mainly used clinically to treat diseases such as liver and kidney yin deficiency, soreness of the waist and knees, dizziness, dizziness, excessive tears, and consumptive cough. Lycium barbarum polysaccharide is the main material basis for its pharm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 高向东刘玮徐金楠姚文兵
Owner CHINA PHARM UNIV
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