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Primary hypertension susceptibility gene variation locus and detecting method thereof

A technology for essential hypertension and high blood pressure, applied in the fields of molecular biology and medicine, can solve problems such as decreased expression, abnormal expression regulation, and less research

Inactive Publication Date: 2015-03-25
BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Claims
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Problems solved by technology

[0006] Since the main characteristic of Mfn2 gene in the blood vessels and leukocytes of patients with essential hypertension and spontaneously hypertensive rats is the decreased expression, this should be due to the abnormality of its expression regulation. few

Method used

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  • Primary hypertension susceptibility gene variation locus and detecting method thereof

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Embodiment 1

[0034] The -1812bp to -1813bp sites of the 5' non-coding region of the essential hypertension susceptibility gene MFn2 gene were detected by sequencing. 30 cases of the above-mentioned hypertension case-control samples were selected for sequencing to determine the genotypes of -1812bp to -1813bp in the 5' non-coding region.

[0035] 1. Experimental method

[0036] The above PCR primers were still used as primers for PCR sequencing, and the amplified products were directly sequenced after purification. The sequencing instrument is ABI's 3130xl genetic analyzer, which is analyzed with sequence analysis 5.2 analysis software, and the results can also be viewed with chromas.

[0037] 2. Experimental results

[0038] The screenshot of the sequencing result is as follows: figure 1 shown.

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Abstract

The invention discloses a primary hypertension susceptibility relevant locus and a method for detecting the single-nucleotide polymorphism of a primary hypertension relevant gene- mitofusin 2 gene(Mfn2 gene) / hyperplasia suppressor gene (HSG), wherein the detecting method comprises detecting the genotypes of basic groups between -945bp and -946bp in a non-coding region at the 5' end of the individual mitofusin 2 (Mfn2) / hyperplasia suppressor gene (HSG). The invention also discloses a corresponding detecting kit.

Description

[0001] technical field [0002] The present invention relates to the fields of molecular biology and medicine. More specifically, it involves the detection of the non-coding region -1812bp to -1813bp at the 5' end of the single nucleotide polymorphism (single nucleotide polymorphism, SNP) site of human mitochondrial fusion gene 2 (Mitofusin 2, Mfn2 gene). The invention also relates to a kit for detecting the SNP site. Background technique [0003] Essential hypertension (essential hypertension, EH) is a common and frequently occurring multifactorial and polygenic disease caused by both environmental and genetic factors, which has had a great impact on human health. With the development of molecular medicine, more than 150 hypertension-related genes have been discovered, but the pathogenesis of EH is still not completely clear, and the early diagnosis and prevention of hypertension have not been fully resolved. EH is the result of the joint action of genetic factors and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 王佐广魏永祥李梅刘洁琳刘雅文杰牛秋丽彭晓云温绍君
Owner BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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