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Cell production method for influenza virus vaccine

A technology for influenza virus and production method, which is applied in the field of cell production of influenza virus vaccine, can solve the problems of hidden danger of vaccine safety and excessive production cycle, and achieve the effects of reducing production cost, short production cycle and easy operation.

Inactive Publication Date: 2015-03-25
CHENGDU VERO BIOTECH
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a cell production method of influenza virus vaccine, so as to overcome the problems that the production cycle of the existing influenza virus vaccine preparation method is too long and the prepared vaccine has potential safety hazards

Method used

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  • Cell production method for influenza virus vaccine

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Embodiment

[0024] Such as figure 1 Shown, the cell production method of influenza virus vaccine is characterized in that, comprises the following steps:

[0025] 1) Preparation of seed poison

[0026] a) Passage and culture of cells: Digest and passage Vero cells with Thermo scientific HyQTase-trypsin cell dispersion, and continue to culture with HycloneSFM4MegaVir or equivalent serum-free medium cell growth medium to form monolayer cells;

[0027] b) Inoculation and propagation of seed virus: use cell maintenance solution to dilute the H1, H3 and B-type epidemic strains provided by WHO in 10 -4 Inoculate the well-growing monolayer cells above, and add 3ug / L Type IX trypsin at the same time, continue to cultivate, and harvest the virus liquid when the cells are more than 50--100% diseased; then use the virus liquid as a seed virus on the cells Carry out cell adaptive continuous passage for 6 generations as production seeds;

[0028] 2) Microcarrier suspension culture of Vero cells in ...

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Abstract

The invention discloses a cell production method for an influenza virus vaccine. The cell production method comprises the following steps: preparing of virus-seeds, microcarrier suspension culture of a Vero cell in a bioreactor, breeding of virus liquid for preparing the vaccine, and inactivation and purification of the virus liquid. According to the invention, chick embryo tissue is replaced with cells for culturing and preparing the influenza virus vaccine, so that the problems of chick embryo pollution and exogenous virus pollution to the chick embryo are solved; through the strict control over the raw materials and the culture conditions, that the produced vaccine is pure can be ensured and the safety of the vaccine is ensured; the bioreactor is used for producing the vaccine, so that advantages of high degree of automation, labor saving and simple and stable production processes are achieved; the production cost can be reduced greatly, the raw material supply limitation does not exist, and the production cycle is short; by using the method for producing the vaccine, less environmental pollution and high easiness in treatment are achieved, and the problems of a great amount of generated waste, high difficulty in treatment, biosafety and public health of the conventional chick embryo production method are solved.

Description

technical field [0001] The invention relates to the field of influenza virus vaccines, in particular to a cell production method of influenza virus vaccines. Background technique [0002] The traditional method for preparing influenza virus vaccines is to use chicken embryos, which have been used in China for more than 50 years to cultivate vaccines. Currently used influenza vaccines are mainly inactivated influenza trivalent split vaccines prepared from chicken embryos, including influenza A strains, H3N2 and influenza B strains, and pandemic influenza vaccines. The main source of sick vaccine strains is determined by the surface antigens of influenza virus epidemic strains recommended by WHO according to the annual change of global influenza viruses for the production of influenza vaccines in the next year. The epidemic wild strains recommended by WHO and chicken embryos Double infection of high-yielding strains produces reassortment or anti-genetic reassortment, and new...

Claims

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Application Information

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IPC IPC(8): A61K39/145A61P31/16C12N7/04
Inventor 高鹏左红代荣涛杨秦
Owner CHENGDU VERO BIOTECH
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