Anaerobic enrichment medium and preparation method thereof

Abstract

The invention belongs to the technical field of microbial culture, and concretely relates to an anaerobic enrichment medium and a preparation method thereof. The medium includes peptone, yeast extract powder, a brain and heart infusion broth, glucose, sodium thioglycolate, hemin, vitamin K1, an anticoagulant, an anaerobic indicator and distilled water; and the medium for promoting mass propagation of anaerobic bacteria can be prepared through a special ratio of the above components. The medium is used to clinically culture anaerobic pathogens in order to improve the positive detection rate of the anaerobic pathogens.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to an anaerobic bacteria enrichment medium and a preparation method thereof. Background technique [0002] Bacteremia caused by anaerobic bacteria has been on the rise in recent years and causes a high mortality rate. In the 1970s, the isolation rate of anaerobic bacteria in blood culture was as high as 20% to 30%, of which Bacteroides fragilis accounted for 78%, second only to Escherichia coli among Gram-negative bacteria. In recent years, the emergence of drug resistance of anaerobic bacteria has challenged empirical treatment, and the clinical infection and clinical consequences caused by anaerobic bacteria have received renewed attention. Anaerobic blood culture is the gold standard method for rapid and accurate diagnosis of anaerobic bacteremia. [0003] Retrospective studies on anaerobic bacteremia show that the mortality rate of anaerobic bacte...

AI Technical Summary

Problems solved by technology

Due to the harsh requirements of anaerobic bacteria on environmental conditions, the growth rate is slow, and the laboratory report results take a long time, which cannot provide timely clinical guidance